Experiment description of studies that identified EIF6 in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 191 ISEV standards ✘ EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD81|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 217 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD81|CD9|CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 63 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ AQP2 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for EIF6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PDHA1 5160 Two-hybrid Homo sapiens 2 FHL2   Invitro Homo sapiens Two-hybrid Homo sapiens 3 NPEPPS 9520 Affinity Capture-MS Homo sapiens 4 Sep-03   Two-hybrid Homo sapiens 5 WFS1   Two-hybrid Homo sapiens 6 HAS1 3036 Affinity Capture-MS Homo sapiens 7 FLJ10094   Two-hybrid Homo sapiens 8 RPL6 6128 Two-hybrid Homo sapiens 9 SORBS1 10580 Affinity Capture-MS Homo sapiens 10 CRELD1   Two-hybrid Homo sapiens 11 EIF3C 8663 Co-purification Homo sapiens 12 RIF1   Two-hybrid Homo sapiens 13 FLJ20534   Two-hybrid Homo sapiens 14 ALDH2 217 Two-hybrid Homo sapiens 15 ABCF1 23 Two-hybrid Homo sapiens 16 PAICS 10606 Affinity Capture-MS Homo sapiens 17 CGI-115   Affinity Capture-MS Homo sapiens 18 FUNDC2   Two-hybrid Homo sapiens 19 YARS 8565 Affinity Capture-MS Homo sapiens 20 RPL3 6122 Affinity Capture-MS Homo sapiens 21 XRN2 22803 Affinity Capture-MS Homo sapiens 22 ARF6 382 Affinity Capture-MS Homo sapiens 23 FUS 2521 Affinity Capture-MS Homo sapiens 24 PSME1 5720 Two-hybrid Homo sapiens 25 RPL34 6164 Affinity Capture-MS Homo sapiens 26 OAT 4942 Affinity Capture-MS Homo sapiens 27 ACAP3 116983 Two-hybrid Homo sapiens 28 POLA2   Two-hybrid Homo sapiens 29 OFD1 8481 Two-hybrid Homo sapiens 30 FIBP   Affinity Capture-MS Homo sapiens 31 AKT1S1 84335 Two-hybrid Homo sapiens 32 LOC387753   Affinity Capture-MS Homo sapiens 33 MRPS31   Two-hybrid Homo sapiens 34 KIAA1377   Two-hybrid Homo sapiens 35 ITGB4 3691 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 36 BCCIP 56647 Affinity Capture-MS Homo sapiens 37 EIF3B 8662 Co-purification Homo sapiens 38 EIF3J 8669 Co-purification Homo sapiens 39 HIP1 3092 Two-hybrid Homo sapiens 40 GIT1 28964 Two-hybrid Homo sapiens 41 GNB2L1 10399 Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 42 RGS10 6001 Affinity Capture-MS Homo sapiens 43 PRKCB 5579 Affinity Capture-MS Homo sapiens 44 UPF3B   Two-hybrid Homo sapiens 45 ACTG1 71 Two-hybrid Homo sapiens 46 GTPBP4 23560 Affinity Capture-MS Homo sapiens 47 USP33   Two-hybrid Homo sapiens View the network

Pathways in which EIF6 is involved

No pathways found