Gene description for DDX20
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Gene symbol DDX20
Other names/aliases DP103
GEMIN3
Species Homo sapiens
 Database cross references - DDX20
ExoCarta ExoCarta_11218
Vesiclepedia VP_11218
Entrez Gene 11218
HGNC 2743
MIM 606168
UniProt Q9UHI6  
 DDX20 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DDX20
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IEA
    RNA helicase activity GO:0003724 IBA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein domain specific binding GO:0019904 IEA
    protein-macromolecule adaptor activity GO:0030674 IEA
    histone deacetylase binding GO:0042826 IEA
    cohesin loader activity GO:0061775 IEA
    DNA-binding transcription factor binding GO:0140297 IPI
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    spliceosomal tri-snRNP complex assembly GO:0000244 TAS
    spliceosomal snRNP assembly GO:0000387 EXP
    spliceosomal snRNP assembly GO:0000387 IBA
    spliceosomal snRNP assembly GO:0000387 IDA
    chromatin remodeling GO:0006338 IEA
    RNA processing GO:0006396 TAS
    negative regulation of cell population proliferation GO:0008285 IEA
    positive regulation of apoptotic process GO:0043065 IGI
    positive regulation of apoptotic process GO:0043065 ISS
    oogenesis GO:0048477 IEA
    regulation of steroid biosynthetic process GO:0050810 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    membrane GO:0016020 HDA
    nuclear body GO:0016604 IDA
    SMN complex GO:0032797 IBA
    SMN complex GO:0032797 IDA
    SMN complex GO:0032797 IPI
    SMN-Sm protein complex GO:0034719 IDA
    RNA polymerase II transcription repressor complex GO:0090571 IEA
    Gemini of coiled bodies GO:0097504 IEA
 Experiment description of studies that identified DDX20 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DDX20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RN7SL1 6029
Protein-RNA Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 MCM3 4172
Affinity Capture-MS Homo sapiens
5 NCBP1 4686
Affinity Capture-MS Homo sapiens
6 SNRPG 6637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 UBL4A 8266
Affinity Capture-MS Homo sapiens
8 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 COIL  
Proximity Label-MS Homo sapiens
10 STAT5A 6776
Affinity Capture-MS Homo sapiens
11 HCST  
Affinity Capture-MS Homo sapiens
12 FA2H  
Affinity Capture-MS Homo sapiens
13 EIF2AK1  
Affinity Capture-MS Homo sapiens
14 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PTPN9 5780
Affinity Capture-MS Homo sapiens
16 USP9X 8239
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 GEMIN8 54960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 COPA 1314
Affinity Capture-Western Homo sapiens
19 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 SNRPD2 6633
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
22 SRP54 6729
Affinity Capture-Western Homo sapiens
23 TCF3  
Affinity Capture-MS Homo sapiens
24 SMN2 6607
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
25 Kcnk1  
Affinity Capture-MS Mus musculus
26 RNPS1 10921
Affinity Capture-MS Homo sapiens
27 B3GNT2 10678
Affinity Capture-MS Homo sapiens
28 USO1 8615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KIF23 9493
Affinity Capture-MS Homo sapiens
30 COPS5 10987
Affinity Capture-MS Homo sapiens
31 CPSF7 79869
Affinity Capture-MS Homo sapiens
32 SNRPC 6631
Affinity Capture-MS Homo sapiens
33 POU5F1  
Affinity Capture-MS Homo sapiens
34 FBL 2091
Affinity Capture-Western Homo sapiens
35 POLR1E  
Affinity Capture-MS Homo sapiens
36 Snrnp70 20637
Affinity Capture-MS Mus musculus
37 TACSTD2 4070
Affinity Capture-MS Homo sapiens
38 SNRPD3 6634
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 POLR2C 5432
Proximity Label-MS Homo sapiens
40 PFN2 5217
Affinity Capture-MS Homo sapiens
41 NPAS1  
Affinity Capture-MS Homo sapiens
42 GPR17 2840
Affinity Capture-MS Homo sapiens
43 GEMIN2 8487
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 KIF14 9928
Affinity Capture-MS Homo sapiens
45 GAR1 54433
Affinity Capture-Western Homo sapiens
46 LSM2 57819
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 AGO2 27161
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 PFN1 5216
Proximity Label-MS Homo sapiens
49 GEMIN5 25929
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SRP9 6726
Affinity Capture-Western Homo sapiens
51 RNU2-1  
Protein-RNA Homo sapiens
52 POLD1 5424
Affinity Capture-MS Homo sapiens
53 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
54 ITGA6 3655
Cross-Linking-MS (XL-MS) Homo sapiens
55 RB1CC1 9821
Affinity Capture-MS Homo sapiens
56 PARK2  
Affinity Capture-MS Homo sapiens
57 WHSC1 7468
Affinity Capture-MS Homo sapiens
58 PHAX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PNKP 11284
Affinity Capture-MS Homo sapiens
60 CLNS1A 1207
Affinity Capture-MS Homo sapiens
61 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SNRPB 6628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
63 SNRPD1 6632
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 BARD1 580
Affinity Capture-MS Homo sapiens
65 ARRDC2  
Affinity Capture-MS Homo sapiens
66 NTRK1 4914
Affinity Capture-MS Homo sapiens
67 NPTN 27020
Affinity Capture-MS Homo sapiens
68 EAF1  
Affinity Capture-MS Homo sapiens
69 SNRPN 6638
Affinity Capture-MS Homo sapiens
70 CRY2  
Affinity Capture-MS Homo sapiens
71 DHX9 1660
Affinity Capture-Western Homo sapiens
72 GEMIN7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 AP3B1 8546
Affinity Capture-MS Homo sapiens
74 RNU1-1 26871
Protein-RNA Homo sapiens
75 ITCH 83737
Affinity Capture-Western Homo sapiens
76 HDAC5 10014
Affinity Capture-MS Homo sapiens
77 FAXC  
Affinity Capture-MS Homo sapiens
78 SNRPB2 6629
Affinity Capture-MS Homo sapiens
79 RBM39 9584
Affinity Capture-MS Homo sapiens
80 CLN3 1201
Affinity Capture-MS Homo sapiens
81 PPP4R2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
82 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 FGF14  
Affinity Capture-MS Homo sapiens
84 RC3H2  
Affinity Capture-MS Homo sapiens
85 ECT2 1894
Affinity Capture-MS Homo sapiens
86 SIRT7  
Affinity Capture-MS Homo sapiens
87 MTX2 10651
Proximity Label-MS Homo sapiens
88 CD2BP2 10421
Affinity Capture-MS Homo sapiens
89 CRYAB 1410
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
90 CUL3 8452
Affinity Capture-MS Homo sapiens
91 SLX1B  
Affinity Capture-MS Homo sapiens
92 EGFR 1956
Affinity Capture-MS Homo sapiens
93 DCLRE1C  
Affinity Capture-MS Homo sapiens
94 FUS 2521
Affinity Capture-MS Homo sapiens
95 NAF1  
Affinity Capture-MS Homo sapiens
96 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
97 STRAP 11171
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 GIGYF2 26058
Affinity Capture-MS Homo sapiens
99 GEMIN4 50628
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 MFAP1  
Affinity Capture-MS Homo sapiens
101 MYO6 4646
Affinity Capture-MS Homo sapiens
102 MYC  
Affinity Capture-MS Homo sapiens
103 RPA3 6119
Proximity Label-MS Homo sapiens
104 LSM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 SCN2B  
Affinity Capture-MS Homo sapiens
106 DDRGK1 65992
Affinity Capture-MS Homo sapiens
107 P2RY10  
Affinity Capture-MS Homo sapiens
108 HAX1  
Proximity Label-MS Homo sapiens
109 ASXL1  
Affinity Capture-MS Homo sapiens
110 NANOG  
Affinity Capture-MS Homo sapiens
111 PARP16 54956
Affinity Capture-MS Homo sapiens
112 ATRX 546
Affinity Capture-MS Homo sapiens
113 P2RY12 64805
Affinity Capture-MS Homo sapiens
114 RBM14 10432
Affinity Capture-MS Homo sapiens
115 GSK3B 2932
Affinity Capture-MS Homo sapiens
116 PHB2 11331
Affinity Capture-MS Homo sapiens
117 ALDH3A2 224
Affinity Capture-MS Homo sapiens
118 PRRC2A 7916
Two-hybrid Homo sapiens
119 SMN1 6606
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
120 CPSF6 11052
Affinity Capture-MS Homo sapiens
121 TP53 7157
Affinity Capture-MS Homo sapiens
122 SNUPN 10073
Reconstituted Complex Homo sapiens
123 MFSD4  
Affinity Capture-MS Homo sapiens
124 GEMIN6  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 TPTE  
Affinity Capture-MS Homo sapiens
126 PLCD3 113026
Affinity Capture-MS Homo sapiens
127 SNRPA 6626
Affinity Capture-MS Homo sapiens
128 MAPRE3  
Proximity Label-MS Homo sapiens
129 PDCD6IP 10015
Cross-Linking-MS (XL-MS) Homo sapiens
130 UFL1 23376
Affinity Capture-MS Homo sapiens
131 SNRNP70 6625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 PXN 5829
Proximity Label-MS Homo sapiens
133 RDX 5962
Affinity Capture-MS Homo sapiens
134 CIT 11113
Affinity Capture-MS Homo sapiens
135 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
136 C9orf72  
Affinity Capture-MS Homo sapiens
137 PRMT1 3276
Affinity Capture-MS Homo sapiens
138 Smn1 20595
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here