Gene ontology annotations for TRAP1
Experiment description of studies that identified TRAP1 in sEVs
1
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
412
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
6
Experiment ID
414
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
7
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
10
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
20
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
12
Experiment ID
407
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
13
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
15
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
17
Experiment ID
405
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
18
Experiment ID
417
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
19
Experiment ID
411
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
20
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
21
Experiment ID
488
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
22
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
23
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
24
Experiment ID
224
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
25
Experiment ID
413
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
26
Experiment ID
406
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
27
Experiment ID
415
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
28
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
29
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
30
Experiment ID
408
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
31
Experiment ID
409
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
32
Experiment ID
416
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
33
Experiment ID
142
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
LNCaP - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
34
Experiment ID
143
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
LNCaP - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
35
Experiment ID
274
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
36
Experiment ID
410
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
Protein-protein interactions for TRAP1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
FAN1
Affinity Capture-MS
Homo sapiens
2
CBS
875
Affinity Capture-MS
Homo sapiens
3
PDHA1
5160
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
4
UBE2H
7328
Affinity Capture-MS
Homo sapiens
5
ISG15
9636
Affinity Capture-MS
Homo sapiens
6
OXCT1
5019
Co-fractionation
Homo sapiens
7
EIF2AK4
440275
Affinity Capture-Western
Homo sapiens
8
DNAJC11
55735
Affinity Capture-MS
Homo sapiens
9
CLIP1
6249
Affinity Capture-MS
Homo sapiens
10
IDH1
3417
Co-fractionation
Homo sapiens
11
MAGEA3
Affinity Capture-MS
Homo sapiens
12
DNAJC8
22826
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
13
IQGAP1
8826
Co-fractionation
Homo sapiens
14
COQ3
Co-fractionation
Homo sapiens
15
SAMM50
25813
Affinity Capture-MS
Homo sapiens
16
AKAP12
9590
Affinity Capture-MS
Homo sapiens
17
PKM
5315
Co-fractionation
Homo sapiens
18
PSMA4
5685
Cross-Linking-MS (XL-MS)
Homo sapiens
19
RPA2
6118
Proximity Label-MS
Homo sapiens
20
LAP3
51056
Co-fractionation
Homo sapiens
21
MKNK2
2872
Affinity Capture-MS
Homo sapiens
22
APC
Affinity Capture-MS
Homo sapiens
23
RPS19
6223
Co-fractionation
Homo sapiens
24
LGALS1
3956
Affinity Capture-MS
Homo sapiens
25
PHOSPHO1
Affinity Capture-MS
Homo sapiens
26
UBC
7316
Affinity Capture-MS
Homo sapiens
27
EIF4A1
1973
Affinity Capture-Western
Homo sapiens
28
VAMP2
6844
Co-fractionation
Homo sapiens
29
CFL1
1072
Co-fractionation
Homo sapiens
30
GLRX
2745
Co-fractionation
Homo sapiens
31
PARK7
11315
Affinity Capture-MS
Homo sapiens
32
UBE2N
7334
Affinity Capture-MS
Homo sapiens
33
RTN4
57142
Affinity Capture-MS
Homo sapiens
34
SOX2
Affinity Capture-MS
Homo sapiens
35
APP
351
Reconstituted Complex
Homo sapiens
36
CCDC90B
Proximity Label-MS
Homo sapiens
37
HSPA5
3309
Co-fractionation
Homo sapiens
38
LGALS3BP
3959
Affinity Capture-MS
Homo sapiens
39
KIAA1429
25962
Affinity Capture-MS
Homo sapiens
40
C9orf78
51759
Affinity Capture-MS
Homo sapiens
41
DYNC1H1
1778
Affinity Capture-MS
Homo sapiens
42
HEATR3
55027
Affinity Capture-MS
Homo sapiens
43
DUT
1854
Co-fractionation
Homo sapiens
44
PRC1
9055
Affinity Capture-MS
Homo sapiens
45
KIF23
9493
Affinity Capture-MS
Homo sapiens
46
SF3B1
23451
Co-fractionation
Homo sapiens
47
CALD1
800
Affinity Capture-MS
Homo sapiens
48
AI837181
Affinity Capture-MS
Mus musculus
49
HGS
9146
Reconstituted Complex
Homo sapiens
50
MRPL12
6182
Affinity Capture-MS
Homo sapiens
51
LTA4H
4048
Co-fractionation
Homo sapiens
52
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
53
EIF5B
9669
Affinity Capture-MS
Homo sapiens
54
RER1
11079
Affinity Capture-MS
Homo sapiens
55
SF3A2
8175
Affinity Capture-MS
Homo sapiens
56
MAGED2
10916
Co-fractionation
Homo sapiens
57
PGD
5226
Co-fractionation
Homo sapiens
58
SRP68
6730
Affinity Capture-MS
Homo sapiens
59
BLVRB
645
Affinity Capture-MS
Homo sapiens
60
ATG16L1
55054
Affinity Capture-MS
Homo sapiens
61
TFCP2
7024
Affinity Capture-MS
Homo sapiens
62
ARIH2
10425
Affinity Capture-MS
Homo sapiens
63
USP7
7874
Co-fractionation
Homo sapiens
64
PLS3
5358
Affinity Capture-MS
Homo sapiens
65
SOD1
6647
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
66
EEF1A1
1915
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
67
CPNE3
8895
Affinity Capture-MS
Homo sapiens
68
KIF14
9928
Affinity Capture-MS
Homo sapiens
69
ALDOA
226
Co-fractionation
Homo sapiens
70
KDF1
Affinity Capture-MS
Homo sapiens
71
RB1
5925
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
72
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
73
PFKM
5213
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-localization
Homo sapiens
74
DLD
1738
Affinity Capture-MS
Homo sapiens
75
PRDX5
25824
Co-fractionation
Homo sapiens
76
COX15
1355
Affinity Capture-MS
Homo sapiens
77
FASTKD5
Proximity Label-MS
Homo sapiens
78
ZNF207
7756
Affinity Capture-MS
Homo sapiens
79
PARK2
Affinity Capture-MS
Homo sapiens
80
CDK5
1020
Affinity Capture-MS
Homo sapiens
81
EEF1G
1937
Affinity Capture-Western
Homo sapiens
82
RPE
Co-fractionation
Homo sapiens
83
NOP2
4839
Affinity Capture-MS
Homo sapiens
84
UBR5
51366
Affinity Capture-MS
Homo sapiens
85
CHMP4B
128866
Affinity Capture-MS
Homo sapiens
86
NELFE
7936
Affinity Capture-MS
Homo sapiens
87
YAP1
10413
Affinity Capture-MS
Homo sapiens
88
MYH9
4627
Affinity Capture-MS
Homo sapiens
89
TYK2
7297
Co-fractionation
Homo sapiens
90
NTRK1
4914
Affinity Capture-MS
Homo sapiens
91
CRKL
1399
Affinity Capture-MS
Homo sapiens
92
FBXL6
Affinity Capture-MS
Homo sapiens
93
BRAF
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
94
CEP72
Affinity Capture-MS
Homo sapiens
95
SND1
27044
Affinity Capture-MS
Homo sapiens
96
HDAC5
10014
Affinity Capture-MS
Homo sapiens
97
VDAC2
7417
Co-fractionation
Homo sapiens
98
CYLC2
Cross-Linking-MS (XL-MS)
Homo sapiens
99
PRKACB
5567
Affinity Capture-MS
Homo sapiens
100
RAD21
5885
Affinity Capture-Western
Homo sapiens
101
SF3A3
10946
Affinity Capture-MS
Homo sapiens
102
GSR
2936
Co-fractionation
Homo sapiens
103
SNRPB2
6629
Affinity Capture-MS
Homo sapiens
104
ANLN
54443
Affinity Capture-MS
Homo sapiens
105
GAN
8139
Affinity Capture-MS
Homo sapiens
106
PAX6
Affinity Capture-MS
Homo sapiens
107
TNFAIP8L1
Affinity Capture-MS
Homo sapiens
108
AK2
204
Co-fractionation
Homo sapiens
109
ASS1
445
Affinity Capture-MS
Homo sapiens
110
METTL14
Affinity Capture-MS
Homo sapiens
111
RPL7A
6130
Co-fractionation
Homo sapiens
112
NR3C1
2908
Affinity Capture-MS
Homo sapiens
113
ECT2
1894
Affinity Capture-MS
Homo sapiens
114
DHX15
1665
Co-fractionation
Homo sapiens
115
SNRPB
6628
Co-fractionation
Homo sapiens
116
TRAF3IP1
26146
Affinity Capture-MS
Homo sapiens
117
MEX3A
Affinity Capture-RNA
Homo sapiens
118
GOT1
2805
Co-fractionation
Homo sapiens
119
TNFRSF1A
7132
Reconstituted Complex
Homo sapiens
120
CUL3
8452
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
121
RPUSD3
Proximity Label-MS
Homo sapiens
122
PDIA3
2923
Affinity Capture-MS
Homo sapiens
123
U2SURP
23350
Co-fractionation
Homo sapiens
124
NANS
54187
Affinity Capture-MS
Homo sapiens
125
HSD17B10
3028
Affinity Capture-MS
Homo sapiens
126
FNBP4
Affinity Capture-MS
Homo sapiens
127
FBXO6
26270
Affinity Capture-MS
Homo sapiens
128
COX6B1
1340
Co-fractionation
Homo sapiens
129
RIPK4
Affinity Capture-MS
Homo sapiens
130
ECHS1
1892
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
131
FN1
2335
Affinity Capture-MS
Homo sapiens
132
NXF1
10482
Affinity Capture-RNA
Homo sapiens
133
SF3A1
10291
Affinity Capture-MS
Homo sapiens
134
AURKB
9212
Affinity Capture-MS
Homo sapiens
135
CEP250
11190
Affinity Capture-MS
Homo sapiens
136
EXT1
2131
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
137
DND1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
138
CNR2
Affinity Capture-MS
Homo sapiens
139
BAG1
573
Affinity Capture-MS
Homo sapiens
140
ESD
2098
Affinity Capture-MS
Homo sapiens
141
DLST
1743
Affinity Capture-MS
Homo sapiens
142
HSDL2
84263
Co-fractionation
Homo sapiens
143
AADAT
Co-fractionation
Homo sapiens
144
ACAD9
28976
Proximity Label-MS
Homo sapiens
145
ENO2
2026
Co-fractionation
Homo sapiens
146
CUL7
9820
Affinity Capture-MS
Homo sapiens
147
VDAC1
7416
Affinity Capture-MS
Homo sapiens
148
HSPD1
3329
Co-fractionation
Homo sapiens
149
HSPA9
3313
Cross-Linking-MS (XL-MS)
Homo sapiens
150
MCUR1
63933
Proximity Label-MS
Homo sapiens
151
CCDC8
Affinity Capture-MS
Homo sapiens
152
PHGDH
26227
Affinity Capture-MS
Homo sapiens
153
TPM3
7170
Two-hybrid
Homo sapiens
154
AIMP1
9255
Affinity Capture-MS
Homo sapiens
155
CRNN
49860
Affinity Capture-MS
Homo sapiens
156
PA2G4
5036
Affinity Capture-MS
Homo sapiens
157
H2AFX
3014
Affinity Capture-MS
Homo sapiens
158
CS
1431
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Proximity Label-MS
Homo sapiens
159
KIF2A
3796
Affinity Capture-MS
Homo sapiens
160
ESX1
Two-hybrid
Homo sapiens
161
ALDOC
230
Co-fractionation
Homo sapiens
162
AUH
549
Proximity Label-MS
Homo sapiens
163
BKRF1
Affinity Capture-MS
164
CPSF6
11052
Affinity Capture-MS
Homo sapiens
165
GPI
2821
Affinity Capture-MS
Homo sapiens
166
PYGL
5836
Affinity Capture-MS
Homo sapiens
167
FOLR1
2348
Affinity Capture-MS
Homo sapiens
168
ASNS
440
Co-fractionation
Homo sapiens
169
PCMT1
5110
Co-fractionation
Homo sapiens
170
DDX3X
1654
Affinity Capture-MS
Homo sapiens
171
APEX1
328
Affinity Capture-RNA
Homo sapiens
172
CUL5
8065
Affinity Capture-MS
Homo sapiens
173
RYK
6259
Affinity Capture-MS
Homo sapiens
174
PRKACA
5566
Affinity Capture-MS
Homo sapiens
175
KDM4C
Affinity Capture-MS
Homo sapiens
176
SMURF1
57154
Affinity Capture-MS
Homo sapiens
177
COX8A
Proximity Label-MS
Homo sapiens
178
FKBP5
2289
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
179
OAT
4942
Co-fractionation
Homo sapiens
180
Ppp6r1
Affinity Capture-MS
Mus musculus
181
SFXN1
94081
Proximity Label-MS
Homo sapiens
182
COQ2
Affinity Capture-MS
Homo sapiens
183
IDH2
3418
Affinity Capture-MS
Homo sapiens
184
TXNRD2
10587
Co-fractionation
Homo sapiens
185
OGFR
11054
Co-fractionation
Homo sapiens
186
SOAT1
6646
Affinity Capture-MS
Homo sapiens
187
GAPDH
2597
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
188
EXT2
2132
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
189
AARS2
Proximity Label-MS
Homo sapiens
190
CDK4
1019
Affinity Capture-MS
Homo sapiens
191
CDK9
1025
Affinity Capture-MS
Homo sapiens
192
PINK1
Affinity Capture-MS
Homo sapiens
193
ANXA5
308
Affinity Capture-MS
Homo sapiens
194
CIT
11113
Affinity Capture-MS
Homo sapiens
195
C1QBP
708
Affinity Capture-MS
Homo sapiens
196
SERBP1
26135
Cross-Linking-MS (XL-MS)
Homo sapiens
Affinity Capture-MS
Homo sapiens
197
SNW1
22938
Affinity Capture-MS
Homo sapiens
198
C9orf72
Affinity Capture-MS
Homo sapiens
199
PSMC4
5704
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
FRET
Homo sapiens
200
PTGER4
Two-hybrid
Homo sapiens
201
RPA3
6119
Proximity Label-MS
Homo sapiens
202
WDHD1
Co-fractionation
Homo sapiens
203
ARSF
416
Affinity Capture-MS
Homo sapiens
204
UFM1
51569
Co-fractionation
Homo sapiens
205
MYL12B
103910
Affinity Capture-MS
Homo sapiens
206
CDC5L
988
Affinity Capture-MS
Homo sapiens
207
OBSL1
23363
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which TRAP1 is involved