Gene description for DOPEY2
Gene name dopey family member 2
Gene symbol DOPEY2
Other names/aliases 21orf5
C21orf5
Species Homo sapiens
 Database cross references - DOPEY2
ExoCarta ExoCarta_9980
Vesiclepedia VP_9980
Entrez Gene 9980
HGNC 1291
MIM 604803
UniProt Q9Y3R5  
 DOPEY2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for DOPEY2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    Golgi to endosome transport GO:0006895 ISS
    endoplasmic reticulum organization GO:0007029 ISS
    embryonic pattern specification GO:0009880 IMP
    protein transport GO:0015031 IEA
    cognition GO:0050890 IMP
Subcellular Localization
    Golgi membrane GO:0000139 ISS
    endosome GO:0005768 IBA
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IEA
    early endosome membrane GO:0031901 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified DOPEY2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DOPEY2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MUL1  
Affinity Capture-MS Homo sapiens
2 STK16 8576
Affinity Capture-MS Homo sapiens
3 RAB7A 7879
Proximity Label-MS Homo sapiens
4 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RAB5A 5868
Proximity Label-MS Homo sapiens
6 OPALIN  
Affinity Capture-MS Homo sapiens
7 ECT2 1894
Affinity Capture-MS Homo sapiens
8 MFSD4  
Affinity Capture-MS Homo sapiens
9 PFN1 5216
Proximity Label-MS Homo sapiens
10 HLA-B 3106
Affinity Capture-MS Homo sapiens
11 NPTN 27020
Affinity Capture-MS Homo sapiens
12 TPTE  
Affinity Capture-MS Homo sapiens
13 IGHM 3507
Affinity Capture-MS Homo sapiens
14 MGARP  
Affinity Capture-MS Homo sapiens
15 FPR1  
Affinity Capture-MS Homo sapiens
16 LAMP2 3920
Proximity Label-MS Homo sapiens
17 Sesn2  
Affinity Capture-MS Mus musculus
18 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RAB4A 5867
Proximity Label-MS Homo sapiens
20 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Proximity Label-MS Homo sapiens
22 FOXK2  
Affinity Capture-MS Homo sapiens
23 EGFR 1956
Affinity Capture-MS Homo sapiens
24 ATP1A1 476
Affinity Capture-MS Homo sapiens
25 RAB11A 8766
Proximity Label-MS Homo sapiens
26 GPR182  
Affinity Capture-MS Homo sapiens
27 RAB3B 5865
Proximity Label-MS Homo sapiens
28 CLEC2D  
Affinity Capture-MS Homo sapiens
29 FOXK1 221937
Affinity Capture-MS Homo sapiens
30 RAB9A 9367
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DOPEY2 is involved
No pathways found





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