Gene ontology annotations for PLS1
Experiment description of studies that identified PLS1 in exosomes
1
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
2
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
5
Experiment ID
1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
6
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
7
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
13
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
63
MISEV standards
✘
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for PLS1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
UBE2H
7328
Affinity Capture-MS
Homo sapiens
2
ISG15
9636
Affinity Capture-MS
Homo sapiens
3
LMOD3
Affinity Capture-MS
Homo sapiens
4
MARCKS
4082
Proximity Label-MS
Homo sapiens
5
NKD2
85409
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
6
ACTC1
70
Proximity Label-MS
Homo sapiens
7
CRHBP
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
8
MTMR14
64419
Affinity Capture-MS
Homo sapiens
9
ANKRD39
Affinity Capture-MS
Homo sapiens
10
SOX2
Affinity Capture-MS
Homo sapiens
11
CLIC5
53405
Affinity Capture-MS
Homo sapiens
12
DNAJB6
10049
Affinity Capture-MS
Homo sapiens
13
JAZF1
Affinity Capture-MS
Homo sapiens
14
APEX1
328
Affinity Capture-MS
Homo sapiens
15
RDX
5962
Co-fractionation
Homo sapiens
16
RWDD2B
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
17
GP1BB
2812
Affinity Capture-MS
Homo sapiens
18
PRDX3
10935
Affinity Capture-MS
Homo sapiens
19
ZNF321P
Affinity Capture-MS
Homo sapiens
20
ISYNA1
51477
Co-fractionation
Homo sapiens
21
PRPS1
5631
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
22
KRT31
3881
Affinity Capture-MS
Homo sapiens
23
FAM136A
84908
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
24
PDIA6
10130
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
25
LIPG
9388
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
26
C9orf139
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
27
SOCS3
9021
Affinity Capture-MS
Homo sapiens
28
TIMM50
92609
Affinity Capture-MS
Homo sapiens
29
FKBP14
Co-fractionation
Homo sapiens
30
PLS3
5358
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
31
FKBPL
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
32
DVL2
1856
Affinity Capture-MS
Homo sapiens
33
KIF14
9928
Affinity Capture-MS
Homo sapiens
34
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
35
SPG21
51324
Affinity Capture-MS
Homo sapiens
36
LCP1
3936
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
37
MT2A
4502
Affinity Capture-MS
Homo sapiens
38
CAPZA1
829
Affinity Capture-MS
Homo sapiens
39
GUCY2F
Affinity Capture-MS
Homo sapiens
40
OR10H3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
41
SLAIN1
122060
Affinity Capture-MS
Homo sapiens
42
MYH9
4627
Affinity Capture-MS
Homo sapiens
43
HMGN1
Co-fractionation
Homo sapiens
44
FAM24B
Affinity Capture-MS
Homo sapiens
45
SLC25A32
81034
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
46
ACTB
60
Proximity Label-MS
Homo sapiens
47
AKAP7
9465
Affinity Capture-MS
Homo sapiens
48
SNRNP27
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
49
LYST
1130
Cross-Linking-MS (XL-MS)
Homo sapiens
50
RCAN3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
51
GSX1
Affinity Capture-MS
Homo sapiens
52
ANLN
54443
Affinity Capture-MS
Homo sapiens
53
Myh9
17886
Affinity Capture-MS
Mus musculus
54
TPK1
Affinity Capture-MS
Homo sapiens
55
ECT2
1894
Affinity Capture-MS
Homo sapiens
56
NFKBIA
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
57
CARTPT
Affinity Capture-MS
Homo sapiens
58
ACAD10
Co-fractionation
Homo sapiens
59
GALK2
2585
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
60
SERPINB5
5268
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
61
KRT81
Affinity Capture-MS
Homo sapiens
62
ZMYM6
Affinity Capture-MS
Homo sapiens
63
USP47
55031
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
64
RPL12
6136
Cross-Linking-MS (XL-MS)
Homo sapiens
65
NDC80
10403
Co-fractionation
Homo sapiens
66
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
67
QTRT1
81890
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
68
FYTTD1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
69
TBCA
6902
Affinity Capture-MS
Homo sapiens
70
DHRS3
9249
Affinity Capture-MS
Homo sapiens
71
MSN
4478
Co-fractionation
Homo sapiens
72
CDK10
Affinity Capture-MS
Homo sapiens
73
C10orf32
Affinity Capture-MS
Homo sapiens
74
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
75
SPTAN1
6709
Co-fractionation
Homo sapiens
76
DTD1
92675
Affinity Capture-MS
Homo sapiens
77
PARP1
142
Co-fractionation
Homo sapiens
78
SPRY2
10253
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
79
MTPN
136319
Affinity Capture-MS
Homo sapiens
80
EZR
7430
Co-fractionation
Homo sapiens
81
CXCL14
Affinity Capture-MS
Homo sapiens
82
FSD1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
83
FKBP4
2288
Co-fractionation
Homo sapiens
84
GSKIP
Affinity Capture-MS
Homo sapiens
85
FCGR2A
2212
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
86
PDCD2
5134
Affinity Capture-MS
Homo sapiens
87
METTL6
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
88
INO80C
Affinity Capture-MS
Homo sapiens
89
GSTM3
2947
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
90
CDK13
8621
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
91
MRPL46
Affinity Capture-MS
Homo sapiens
92
CIT
11113
Affinity Capture-MS
Homo sapiens
93
OTUD3
23252
Affinity Capture-MS
Homo sapiens
94
SERBP1
26135
Affinity Capture-MS
Homo sapiens
95
STC2
8614
Affinity Capture-MS
Homo sapiens
96
UBE2Q2
92912
Affinity Capture-MS
Homo sapiens
97
LMO1
Affinity Capture-MS
Homo sapiens
98
CRACR2A
Affinity Capture-MS
Homo sapiens
99
CCR1
Affinity Capture-MS
Homo sapiens
100
CUL4A
8451
Affinity Capture-MS
Homo sapiens
101
CNPPD1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which PLS1 is involved