Gene description for PTPN1
Gene name protein tyrosine phosphatase, non-receptor type 1
Gene symbol PTPN1
Other names/aliases PTP1B
Species Homo sapiens
 Database cross references - PTPN1
ExoCarta ExoCarta_5770
Entrez Gene 5770
HGNC 9642
MIM 176885
UniProt P18031  
 PTPN1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for PTPN1
Molecular Function
    enzyme binding GO:0019899 IPI
    zinc ion binding GO:0008270 IDA
    protein tyrosine phosphatase activity GO:0004725 TAS
    receptor tyrosine kinase binding GO:0030971 IPI
    ephrin receptor binding GO:0046875 IPI
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    insulin receptor binding GO:0005158 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    negative regulation of insulin receptor signaling pathway GO:0046627 NAS
    actin cytoskeleton reorganization GO:0031532 IMP
    positive regulation of protein tyrosine kinase activity GO:0061098 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    cytokine-mediated signaling pathway GO:0019221 TAS
    regulation of signal transduction GO:0009966 IMP
    type I interferon signaling pathway GO:0060337 TAS
    positive regulation of receptor catabolic process GO:2000646 IMP
    regulation of endocytosis GO:0030100 IDA
    regulation of intracellular protein transport GO:0033157 IMP
    regulation of hepatocyte growth factor receptor signaling pathway GO:1902202 IMP
    endoplasmic reticulum unfolded protein response GO:0030968 IDA
    platelet activation GO:0030168 TAS
    negative regulation of MAP kinase activity GO:0043407 IMP
    insulin receptor signaling pathway GO:0008286 IEA
    regulation of type I interferon-mediated signaling pathway GO:0060338 TAS
    peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity GO:1990264 ISS
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    protein dephosphorylation GO:0006470 IMP
    positive regulation of IRE1-mediated unfolded protein response GO:1903896 TAS
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IDA
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 ISS
    blood coagulation GO:0007596 TAS
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    activation of signaling protein activity involved in unfolded protein response GO:0006987 TAS
    peptidyl-tyrosine dephosphorylation GO:0035335 IMP
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    endoplasmic reticulum GO:0005783 IDA
    cytoplasmic vesicle GO:0031410 IEA
    plasma membrane GO:0005886 IDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 IDA
    early endosome GO:0005769 IDA
    sorting endosome GO:0097443 ISS
 Experiment description of studies that identified PTPN1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTPN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDH2 1000
Invitro Homo sapiens
2 PIN1 5300
Affinity Capture-MS Homo sapiens
3 CTNNB1 1499
Invivo Homo sapiens
4 RRAS2 22800
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 NTRK1  
Invitro Homo sapiens
6 NTRK3  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
7 JAK2 3717
Invivo Homo sapiens
8 AKT1 207
Affinity Capture-MS Homo sapiens
9 BCAR1 9564
Invivo Homo sapiens
Invitro Homo sapiens
10 INSR 3643
Invitro Homo sapiens
11 EGFR 1956
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
12 GRB2 2885
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
13 TYK2 7297
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 GSK3B 2932
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
15 CAV1 857
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 NTRK2 4915
Invitro Homo sapiens
17 LTK 4058
Invitro Homo sapiens
18 IGF1R 3480
Invivo Homo sapiens
19 IRS1 3667
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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