Gene description for SPAG9
Gene name sperm associated antigen 9
Gene symbol SPAG9
Other names/aliases CT89
Species Homo sapiens
 Database cross references - SPAG9
ExoCarta ExoCarta_9043
Entrez Gene 9043
HGNC 14524
MIM 605430
UniProt O60271  
 SPAG9 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for SPAG9
Molecular Function
    mitogen-activated protein kinase p38 binding GO:0048273 IEA
    receptor signaling complex scaffold activity GO:0030159 IBA
    MAP-kinase scaffold activity GO:0005078 IBA
    kinesin binding GO:0019894 IBA
    protein binding GO:0005515 IPI
    JUN kinase binding GO:0008432 IBA
Biological Process
    positive regulation of cell migration GO:0030335 IMP
    negative regulation of protein homodimerization activity GO:0090074 IEA
    protein homooligomerization GO:0051260 IEA
    muscle cell differentiation GO:0042692 TAS
    spermatogenesis GO:0007283 TAS
    retrograde transport, endosome to Golgi GO:0042147 IDA
    activation of JUN kinase activity GO:0007257 IBA
    positive regulation of muscle cell differentiation GO:0051149 TAS
    positive regulation of neuron differentiation GO:0045666 IEA
    striated muscle cell differentiation GO:0051146 IEA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    integral component of membrane GO:0016021 TAS
 Experiment description of studies that identified SPAG9 in exosomes
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SPAG9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
2 NFKB1 4790
Affinity Capture-MS Homo sapiens
3 MAPK14 1432
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 MAP2K4 6416
Affinity Capture-Western Homo sapiens
5 MAPK8 5599
Affinity Capture-Western Homo sapiens
6 MAX  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 MAP3K3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SPAG9 is involved
CDO in myogenesis TAS Reactome

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