Gene description for ALPP
Gene name alkaline phosphatase, placental
Gene symbol ALPP
Other names/aliases ALP
PALP
PLAP
PLAP-1
Species Homo sapiens
 Database cross references - ALPP
ExoCarta ExoCarta_250
Vesiclepedia VP_250
Entrez Gene 250
HGNC 439
MIM 171800
UniProt P05187  
 ALPP identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Plasma 17076679    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ALPP
Molecular Function
    magnesium ion binding GO:0000287 ISS
    alkaline phosphatase activity GO:0004035 IBA
    alkaline phosphatase activity GO:0004035 IDA
    alkaline phosphatase activity GO:0004035 NAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell surface GO:0009986 IDA
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified ALPP in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 43
MISEV standards
✔
EM
Biophysical techniques
✔
CD83
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17076679    
Organism Homo sapiens
Experiment description Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences.
Authors "Sabapatha A, Gercel-Taylor C, Taylor DD"
Journal name AJRI
Publication year 2006
Sample Plasma
Sample name Plasma - Pregnant and not-pregnant women
Isolation/purification methods "Size exclusion
Ultracentrifugation
Immunobeads (CD3, CD19, CD56, CD83, PLAP)"
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ALPP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LCE1F  
Two-hybrid Homo sapiens
2 SMCP  
Two-hybrid Homo sapiens
3 LELP1  
Two-hybrid Homo sapiens
4 MAN2B1 4125
Affinity Capture-MS Homo sapiens
5 IDUA 3425
Affinity Capture-MS Homo sapiens
6 NOTCH2NL 388677
Two-hybrid Homo sapiens
7 RPA2 6118
Affinity Capture-MS Homo sapiens
8 KIF20A 10112
Affinity Capture-MS Homo sapiens
9 KRTAP5-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 FBXO34  
Two-hybrid Homo sapiens
11 MGAT5 4249
Affinity Capture-MS Homo sapiens
12 KRTAP4-12  
Two-hybrid Homo sapiens
13 UXS1 80146
Affinity Capture-MS Homo sapiens
14 LCE3C  
Two-hybrid Homo sapiens
15 Trim69  
Affinity Capture-MS Mus musculus
16 KIF23 9493
Affinity Capture-MS Homo sapiens
17 ADGRG1 9289
Affinity Capture-MS Homo sapiens
18 HYAL2 8692
Affinity Capture-MS Homo sapiens
19 Cep76  
Affinity Capture-MS Mus musculus
20 SUN2 25777
Affinity Capture-MS Homo sapiens
21 RGS20 8601
Two-hybrid Homo sapiens
22 PCSK6 5046
Affinity Capture-MS Homo sapiens
23 FAF1 11124
Affinity Capture-MS Homo sapiens
24 GUSB 2990
Affinity Capture-MS Homo sapiens
25 NR4A3  
Two-hybrid Homo sapiens
26 NSFL1C 55968
Affinity Capture-MS Homo sapiens
27 BTRC 8945
Affinity Capture-Western Homo sapiens
28 FAF2 23197
Affinity Capture-MS Homo sapiens
29 Erh  
Affinity Capture-MS Mus musculus
30 P3H4 10609
Affinity Capture-MS Homo sapiens
31 LCE1E  
Two-hybrid Homo sapiens
32 CHID1 66005
Affinity Capture-MS Homo sapiens
33 PALD1 27143
Affinity Capture-MS Homo sapiens
34 ADAMTSL4  
Two-hybrid Homo sapiens
35 LCE2C  
Two-hybrid Homo sapiens
36 CHPF2 54480
Affinity Capture-MS Homo sapiens
37 NOMO1 23420
Affinity Capture-MS Homo sapiens
38 TOR1B 27348
Affinity Capture-MS Homo sapiens
39 P4HTM  
Affinity Capture-MS Homo sapiens
40 LCE3A  
Two-hybrid Homo sapiens
41 ALPI  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 LCE1D  
Two-hybrid Homo sapiens
43 CSPG4 1464
Affinity Capture-MS Homo sapiens
44 CHMP4B 128866
Affinity Capture-MS Homo sapiens
45 CYSRT1 375791
Two-hybrid Homo sapiens
46 JAG2  
Affinity Capture-MS Homo sapiens
47 LCE3E  
Two-hybrid Homo sapiens
48 MEOX2  
Two-hybrid Homo sapiens
49 PCSK9 255738
Affinity Capture-MS Homo sapiens
50 GALNS 2588
Affinity Capture-MS Homo sapiens
51 LCE3B  
Two-hybrid Homo sapiens
52 UBXN6 80700
Affinity Capture-MS Homo sapiens
53 ANLN 54443
Affinity Capture-MS Homo sapiens
54 MEGF8 1954
Affinity Capture-MS Homo sapiens
55 LCE5A  
Two-hybrid Homo sapiens
56 BRF1  
Affinity Capture-MS Homo sapiens
57 KRTAP1-3  
Two-hybrid Homo sapiens
58 MAN2B2 23324
Affinity Capture-MS Homo sapiens
59 ECT2 1894
Affinity Capture-MS Homo sapiens
60 UBXN7 26043
Affinity Capture-MS Homo sapiens
61 LCE3D  
Two-hybrid Homo sapiens
62 KRTAP10-8  
Two-hybrid Homo sapiens
63 METRNL 284207
Affinity Capture-MS Homo sapiens
64 LOXL2 4017
Affinity Capture-MS Homo sapiens
65 IL17RA 23765
Affinity Capture-MS Homo sapiens
66 NAGLU 4669
Affinity Capture-MS Homo sapiens
67 PCYOX1 51449
Affinity Capture-MS Homo sapiens
68 FBXO6 26270
Affinity Capture-MS Homo sapiens
69 KRTAP10-3  
Two-hybrid Homo sapiens
70 HOXA1 3198
Two-hybrid Homo sapiens
71 OTX1  
Two-hybrid Homo sapiens
72 AURKB 9212
Affinity Capture-MS Homo sapiens
73 GEMIN4 50628
Two-hybrid Homo sapiens
74 GALNT18  
Affinity Capture-MS Homo sapiens
75 RPA3 6119
Affinity Capture-MS Homo sapiens
76 KREMEN2  
Affinity Capture-MS Homo sapiens
77 GXYLT1 283464
Affinity Capture-MS Homo sapiens
78 P3H3 10536
Affinity Capture-MS Homo sapiens
79 POGLUT1 56983
Affinity Capture-MS Homo sapiens
80 UBXN1 51035
Affinity Capture-MS Homo sapiens
81 Cbx1  
Affinity Capture-MS Mus musculus
82 CXCL5  
Two-hybrid Homo sapiens
83 NAAA  
Affinity Capture-MS Homo sapiens
84 KIF14 9928
Affinity Capture-MS Homo sapiens
85 FOXRED2  
Affinity Capture-MS Homo sapiens
86 PDIA4 9601
Affinity Capture-MS Homo sapiens
87 RGS19 10287
Two-hybrid Homo sapiens
88 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
89 MGRN1 23295
Affinity Capture-MS Homo sapiens
90 NLGN3  
Two-hybrid Homo sapiens
91 RPA1 6117
Affinity Capture-MS Homo sapiens
92 NBPF19  
Two-hybrid Homo sapiens
93 PIGK 10026
Reconstituted Complex Homo sapiens
94 KRTAP1-1  
Two-hybrid Homo sapiens
95 COL18A1 80781
Affinity Capture-MS Homo sapiens
96 UBXN11  
Affinity Capture-MS Homo sapiens
97 BDNF 627
Affinity Capture-MS Homo sapiens
98 EDEM2  
Affinity Capture-MS Homo sapiens
99 CREB5 9586
Two-hybrid Homo sapiens
100 SEMA3B 7869
Affinity Capture-MS Homo sapiens
101 POU4F2  
Two-hybrid Homo sapiens
102 ALPPL2 251
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 LAMB2 3913
Affinity Capture-MS Homo sapiens
104 IGFBP6 3489
Two-hybrid Homo sapiens
105 ANKRD46 157567
Affinity Capture-MS Homo sapiens
106 ARSK  
Affinity Capture-MS Homo sapiens
107 CRCT1  
Two-hybrid Homo sapiens
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