Gene description for CSNK1A1
Gene name casein kinase 1, alpha 1
Gene symbol CSNK1A1
Other names/aliases CK1
CK1a
CKIa
HEL-S-77p
HLCDGP1
PRO2975
Species Homo sapiens
 Database cross references - CSNK1A1
ExoCarta ExoCarta_1452
Entrez Gene 1452
HGNC 2451
MIM 600505
UniProt P48729  
 CSNK1A1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CSNK1A1
Molecular Function
    protein kinase activity GO:0004672 TAS
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 IDA
Biological Process
    phagocytosis GO:0006909 IBA
    Wnt signaling pathway GO:0016055 IBA
    mitotic nuclear division GO:0007067 IEA
    regulation of cell shape GO:0008360 IBA
    protein phosphorylation GO:0006468 IDA
    peptidyl-serine phosphorylation GO:0018105 IBA
    signal transduction GO:0007165 NAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    cell division GO:0051301 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IBA
    membrane GO:0016020 IDA
    nuclear speck GO:0016607 IDA
    primary cilium GO:0072372 TAS
    centrosome GO:0005813 IDA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IDA
    condensed chromosome kinetochore GO:0000777 IEA
 Experiment description of studies that identified CSNK1A1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSNK1A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AXIN1  
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
2 PPP1R14A  
Co-purification Homo sapiens
3 CENTA1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
4 ACTA1 58
Affinity Capture-MS Homo sapiens
5 SYT9 143425
Co-purification Homo sapiens
6 ERF  
Co-purification Homo sapiens
7 TNFRSF1B  
Invivo Homo sapiens
8 KPNA2 3838
Affinity Capture-MS Homo sapiens
9 HMGB1 3146
Co-purification Homo sapiens
10 RCC1 1104
Co-purification Homo sapiens
11 PPP2CA 5515
Affinity Capture-MS Homo sapiens
12 HMGB2 3148
Co-purification Homo sapiens
13 CHRM3  
Invitro Homo sapiens
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