Gene description for TP53
Gene name tumor protein p53
Gene symbol TP53
Other names/aliases BCC7
LFS1
P53
TRP53
Species Homo sapiens
 Database cross references - TP53
ExoCarta ExoCarta_7157
Vesiclepedia VP_7157
Entrez Gene 7157
HGNC 11998
MIM 191170
UniProt P04637  
 TP53 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for TP53
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISS
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    core promoter sequence-specific DNA binding GO:0001046 IDA
    TFIID-class transcription factor complex binding GO:0001094 IPI
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    protease binding GO:0002020 IPI
    p53 binding GO:0002039 IPI
    DNA binding GO:0003677 IDA
    DNA binding GO:0003677 IMP
    chromatin binding GO:0003682 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    DNA-binding transcription factor activity GO:0003700 IMP
    mRNA 3'-UTR binding GO:0003730 IDA
    copper ion binding GO:0005507 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 TAS
    enzyme binding GO:0019899 IPI
    receptor tyrosine kinase binding GO:0030971 IPI
    ubiquitin protein ligase binding GO:0031625 EXP
    ubiquitin protein ligase binding GO:0031625 IPI
    histone deacetylase regulator activity GO:0035033 IEA
    ATP-dependent DNA/DNA annealing activity GO:0036310 IDA
    identical protein binding GO:0042802 IPI
    histone deacetylase binding GO:0042826 IPI
    protein heterodimerization activity GO:0046982 IPI
    protein-folding chaperone binding GO:0051087 IPI
    protein phosphatase 2A binding GO:0051721 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    14-3-3 protein binding GO:0071889 EXP
    MDM2/MDM4 family protein binding GO:0097371 IEA
    disordered domain specific binding GO:0097718 IPI
    general transcription initiation factor binding GO:0140296 IPI
    molecular function activator activity GO:0140677 EXP
    molecular function activator activity GO:0140677 IPI
    molecular condensate scaffold activity GO:0140693 IDA
    promoter-specific chromatin binding GO:1990841 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    mitophagy GO:0000423 IEA
    in utero embryonic development GO:0001701 IEA
    somitogenesis GO:0001756 IEA
    release of cytochrome c from mitochondria GO:0001836 IEA
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    T cell proliferation involved in immune response GO:0002309 IEA
    B cell lineage commitment GO:0002326 IEA
    T cell lineage commitment GO:0002360 IEA
    response to ischemia GO:0002931 IEA
    nucleotide-excision repair GO:0006289 IMP
    double-strand break repair GO:0006302 IEA
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of DNA-templated transcription GO:0006355 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    protein import into nucleus GO:0006606 IEA
    autophagy GO:0006914 IMP
    DNA damage response GO:0006974 IDA
    DNA damage response GO:0006974 IDA
    DNA damage response GO:0006974 IMP
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 IMP
    DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0006978 IDA
    DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0006978 IMP
    ER overload response GO:0006983 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    Ras protein signal transduction GO:0007265 IEP
    gastrulation GO:0007369 IEA
    neuroblast proliferation GO:0007405 IEA
    negative regulation of neuroblast proliferation GO:0007406 IEA
    protein localization GO:0008104 IDA
    negative regulation of DNA replication GO:0008156 IEA
    negative regulation of cell population proliferation GO:0008285 IDA
    negative regulation of cell population proliferation GO:0008285 IMP
    negative regulation of cell population proliferation GO:0008285 ISS
    negative regulation of cell population proliferation GO:0008285 ISS
    determination of adult lifespan GO:0008340 ISS
    mRNA transcription GO:0009299 IMP
    rRNA transcription GO:0009303 IEA
    response to salt stress GO:0009651 IEA
    response to X-ray GO:0010165 IEA
    response to gamma radiation GO:0010332 IMP
    positive regulation of gene expression GO:0010628 IDA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISS
    cardiac muscle cell apoptotic process GO:0010659 IEA
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 IEA
    glial cell proliferation GO:0014009 IEA
    viral process GO:0016032 IMP
    glucose catabolic process to lactate via pyruvate GO:0019661 IEA
    cerebellum development GO:0021549 IEA
    negative regulation of cell growth GO:0030308 IMP
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IDA
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IMP
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IEA
    mitotic G1 DNA damage checkpoint signaling GO:0031571 IMP
    negative regulation of telomere maintenance via telomerase GO:0032211 IDA
    T cell differentiation in thymus GO:0033077 IEA
    tumor necrosis factor-mediated signaling pathway GO:0033209 IGI
    regulation of tissue remodeling GO:0034103 IEA
    cellular response to UV GO:0034644 IDA
    multicellular organism growth GO:0035264 IEA
    positive regulation of mitochondrial membrane permeability GO:0035794 IEA
    cellular response to glucose starvation GO:0042149 IDA
    cellular response to glucose starvation GO:0042149 IDA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IDA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IDA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    regulation of apoptotic process GO:0042981 IDA
    positive regulation of apoptotic process GO:0043065 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 IGI
    negative regulation of apoptotic process GO:0043066 IMP
    entrainment of circadian clock by photoperiod GO:0043153 ISS
    mitochondrial DNA repair GO:0043504 IEA
    regulation of DNA damage response, signal transduction by p53 class mediator GO:0043516 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    transcription initiation-coupled chromatin remodeling GO:0045815 IDA
    negative regulation of proteolysis GO:0045861 IEA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 IMP
    negative regulation of DNA-templated transcription GO:0045892 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of RNA polymerase II transcription preinitiation complex assembly GO:0045899 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    response to antibiotic GO:0046677 IEP
    fibroblast proliferation GO:0048144 IEA
    negative regulation of fibroblast proliferation GO:0048147 IMP
    circadian behavior GO:0048512 ISS
    bone marrow development GO:0048539 IMP
    embryonic organ development GO:0048568 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISS
    protein stabilization GO:0050821 IEA
    negative regulation of helicase activity GO:0051097 TAS
    protein tetramerization GO:0051262 IEA
    chromosome organization GO:0051276 IEA
    neuron apoptotic process GO:0051402 IEA
    regulation of cell cycle GO:0051726 IDA
    regulation of cell cycle GO:0051726 IGI
    regulation of cell cycle GO:0051726 IMP
    regulation of cell cycle GO:0051726 ISS
    hematopoietic stem cell differentiation GO:0060218 IMP
    negative regulation of glial cell proliferation GO:0060253 IEA
    type II interferon-mediated signaling pathway GO:0060333 IEA
    cardiac septum morphogenesis GO:0060411 IEA
    positive regulation of programmed necrotic cell death GO:0062100 IEA
    protein-containing complex assembly GO:0065003 IDA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IEA
    thymocyte apoptotic process GO:0070242 IEA
    positive regulation of thymocyte apoptotic process GO:0070245 ISS
    necroptotic process GO:0070266 IEA
    cellular response to hypoxia GO:0071456 IEP
    cellular response to xenobiotic stimulus GO:0071466 IEP
    cellular response to ionizing radiation GO:0071479 IMP
    cellular response to gamma radiation GO:0071480 IDA
    cellular response to UV-C GO:0071494 IEA
    stem cell proliferation GO:0072089 IEA
    signal transduction by p53 class mediator GO:0072331 IDA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
    reactive oxygen species metabolic process GO:0072593 IEA
    cellular response to actinomycin D GO:0072717 IDA
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    cellular senescence GO:0090398 IMP
    replicative senescence GO:0090399 IMP
    oxidative stress-induced premature senescence GO:0090403 IMP
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    oligodendrocyte apoptotic process GO:0097252 IDA
    positive regulation of execution phase of apoptosis GO:1900119 IMP
    negative regulation of mitophagy GO:1901525 IEA
    regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902108 IEA
    regulation of intrinsic apoptotic signaling pathway by p53 class mediator GO:1902253 IEA
    regulation of cell cycle G2/M phase transition GO:1902749 IMP
    positive regulation of miRNA transcription GO:1902895 IDA
    positive regulation of miRNA transcription GO:1902895 IGI
    positive regulation of miRNA transcription GO:1902895 IMP
    positive regulation of miRNA transcription GO:1902895 IMP
    negative regulation of G1 to G0 transition GO:1903451 IDA
    negative regulation of G1 to G0 transition GO:1903451 IMP
    negative regulation of miRNA processing GO:1903799 IEA
    negative regulation of glucose catabolic process to lactate via pyruvate GO:1904024 IEA
    negative regulation of pentose-phosphate shunt GO:1905856 IMP
    intrinsic apoptotic signaling pathway in response to hypoxia GO:1990144 IEA
    regulation of fibroblast apoptotic process GO:2000269 IEA
    negative regulation of reactive oxygen species metabolic process GO:2000378 IEA
    positive regulation of reactive oxygen species metabolic process GO:2000379 IMP
    negative regulation of stem cell proliferation GO:2000647 IEA
    positive regulation of cellular senescence GO:2000774 IDA
    positive regulation of cellular senescence GO:2000774 ISS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IMP
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISS
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 IMP
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    replication fork GO:0005657 IEA
    transcription regulator complex GO:0005667 IGI
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IEA
    endoplasmic reticulum GO:0005783 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nuclear matrix GO:0016363 IDA
    nuclear body GO:0016604 IDA
    PML body GO:0016605 IDA
    transcription repressor complex GO:0017053 IPI
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 IMP
    site of double-strand break GO:0035861 IEA
    germ cell nucleus GO:0043073 IEA
 Experiment description of studies that identified TP53 in exosomes
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TP53
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Co-fractionation Homo sapiens
2 NPEPPS 9520
Affinity Capture-MS Homo sapiens
3 AGT 183
Two-hybrid Homo sapiens
4 ZNF24  
Two-hybrid Homo sapiens
5 MED17  
Reconstituted Complex Homo sapiens
6 Atm  
Biochemical Activity Rattus norvegicus
7 SMC5 23137
Affinity Capture-MS Homo sapiens
8 C1orf27 54953
Affinity Capture-MS Homo sapiens
9 TBC1D4  
Affinity Capture-MS Homo sapiens
10 PRPF8 10594
Affinity Capture-MS Homo sapiens
11 TOP2B 7155
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 GADD45A  
Two-hybrid Homo sapiens
13 TTC16  
Affinity Capture-MS Homo sapiens
14 TUBB8 347688
Affinity Capture-MS Homo sapiens
15 SMG7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 UBE3A 7337
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 UBC 7316
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 VHL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 TAF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
20 VASP 7408
Affinity Capture-MS Homo sapiens
21 OTUD3 23252
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 ZNF207 7756
Negative Genetic Homo sapiens
23 MAP1LC3B 81631
Affinity Capture-Western Homo sapiens
24 ARL3 403
Two-hybrid Homo sapiens
Synthetic Growth Defect Homo sapiens
25 RBM28 55131
Affinity Capture-MS Homo sapiens
26 ZNF619  
Synthetic Growth Defect Homo sapiens
27 ACTR3 10096
Proximity Label-MS Homo sapiens
28 OR51M1  
Negative Genetic Homo sapiens
29 BRCC3 79184
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
30 ABHD16B  
Negative Genetic Homo sapiens
31 HDC  
Affinity Capture-MS Homo sapiens
32 RPS9 6203
Affinity Capture-MS Homo sapiens
33 DNAH17 8632
Affinity Capture-MS Homo sapiens
34 KPRP 448834
Affinity Capture-MS Homo sapiens
35 SFN 2810
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 MECP2 4204
Affinity Capture-MS Homo sapiens
37 BTRC 8945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 TFRC 7037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EMC1 23065
Affinity Capture-MS Homo sapiens
40 AGO2 27161
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
41 TGM3 7053
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 AARS 16
Affinity Capture-MS Homo sapiens
43 PPM1B 5495
Affinity Capture-MS Homo sapiens
44 AURKA 6790
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
45 RCC2 55920
Affinity Capture-MS Homo sapiens
46 ANXA7 310
Two-hybrid Homo sapiens
47 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 UTP14A 10813
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
49 SPTA1  
Affinity Capture-MS Homo sapiens
50 LGI4  
Synthetic Growth Defect Homo sapiens
51 CTBP1 1487
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 BRE 9577
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
53 PLD1 5337
Affinity Capture-MS Homo sapiens
54 DAZAP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 ARG1 383
Affinity Capture-MS Homo sapiens
56 YAP1 10413
Affinity Capture-MS Homo sapiens
57 MEGF6  
Affinity Capture-MS Homo sapiens
58 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
59 CD81 975
Affinity Capture-MS Homo sapiens
60 RFC4 5984
Affinity Capture-MS Homo sapiens
61 PRIMA1  
Negative Genetic Homo sapiens
62 CUL2 8453
Affinity Capture-MS Homo sapiens
63 RRP1B 23076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 GTPBP4 23560
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
65 SP100 6672
Affinity Capture-MS Homo sapiens
66 TWIST1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
67 MCOLN2  
Affinity Capture-MS Homo sapiens
68 DDX11L8  
Affinity Capture-MS Homo sapiens
69 HSPA5 3309
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 RBM39 9584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SLC2A1 6513
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
72 HYAL4  
Synthetic Growth Defect Homo sapiens
73 NOC3L 64318
Affinity Capture-MS Homo sapiens
74 CCNL1  
Affinity Capture-MS Homo sapiens
75 SLC5A7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 NPRL3  
Affinity Capture-Western Homo sapiens
77 SIRT7  
Affinity Capture-MS Homo sapiens
78 SCAMP1 9522
Two-hybrid Homo sapiens
79 BCAP31 10134
Affinity Capture-MS Homo sapiens
80 EPRS 2058
Affinity Capture-MS Homo sapiens
81 EIF2S2 8894
Two-hybrid Homo sapiens
82 SIN3B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
83 CNKSR2 22866
Negative Genetic Homo sapiens
84 CDC42 998
Two-hybrid Homo sapiens
85 PUS7L  
Affinity Capture-MS Homo sapiens
86 TUBA4A 7277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 Atr  
Biochemical Activity Rattus norvegicus
89 IFT172 26160
Affinity Capture-MS Homo sapiens
90 GRIN2B  
Synthetic Growth Defect Homo sapiens
91 TIGAR 57103
Co-localization Homo sapiens
92 DZIP1  
Synthetic Growth Defect Homo sapiens
93 RFWD3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 ITPK1 3705
Biochemical Activity Homo sapiens
95 KCTD15 79047
Affinity Capture-MS Homo sapiens
96 EFNB2 1948
Negative Genetic Homo sapiens
97 HIST1H2AJ 8331
Affinity Capture-MS Homo sapiens
98 CEPT1 10390
Affinity Capture-MS Homo sapiens
99 STUB1 10273
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity