Gene description for SRSF8
Gene name serine/arginine-rich splicing factor 8
Gene symbol SRSF8
Other names/aliases DSM-1
SFRS2B
SRP46
Species Homo sapiens
 Database cross references - SRSF8
ExoCarta ExoCarta_10929
Vesiclepedia VP_10929
Entrez Gene 10929
HGNC 16988
MIM 603269
UniProt Q9BRL6  
 SRSF8 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocytes 26054723    
 Gene ontology annotations for SRSF8
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified SRSF8 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for SRSF8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RALY 22913
Affinity Capture-MS Homo sapiens
2 HNRNPC 3183
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 SRPK2 6733
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 PRPF38A 84950
Two-hybrid Homo sapiens
6 RPS19 6223
Affinity Capture-MS Homo sapiens
7 KIF20A 10112
Affinity Capture-MS Homo sapiens
8 ATM 472
Affinity Capture-MS Homo sapiens
9 IL36RN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CCDC9  
Affinity Capture-MS Homo sapiens
11 SRSF2 6427
Cross-Linking-MS (XL-MS) Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
14 EIF4ENIF1 56478
Two-hybrid Homo sapiens
15 SNRNP70 6625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 KIF23 9493
Affinity Capture-MS Homo sapiens
17 WDR83  
Two-hybrid Homo sapiens
18 PRC1 9055
Affinity Capture-MS Homo sapiens
19 SNRPC 6631
Affinity Capture-MS Homo sapiens
20 YWHAG 7532
Affinity Capture-MS Homo sapiens
21 ADARB1 104
Affinity Capture-MS Homo sapiens
22 SNRNP48  
Two-hybrid Homo sapiens
23 EED  
Affinity Capture-MS Homo sapiens
24 Mark2 13728
Affinity Capture-MS Mus musculus
25 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SFN 2810
Affinity Capture-MS Homo sapiens
27 MECP2 4204
Affinity Capture-MS Homo sapiens
28 SNX27 81609
Affinity Capture-MS Homo sapiens
29 SREK1 140890
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 FAM120A 23196
Affinity Capture-MS Homo sapiens
31 CLK3  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
32 LARP4B 23185
Affinity Capture-MS Homo sapiens
33 FOXP1 27086
Protein-RNA Homo sapiens
34 CLK2 1196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
36 ZBTB47  
Affinity Capture-MS Homo sapiens
37 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SRSF6 6431
Affinity Capture-MS Homo sapiens
39 NELFE 7936
Affinity Capture-MS Homo sapiens
40 U2AF2 11338
Affinity Capture-MS Homo sapiens
41 RAD18  
Affinity Capture-MS Homo sapiens
42 RBMX2  
Affinity Capture-MS Homo sapiens
43 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
44 H19 283120
Protein-RNA Homo sapiens
45 MAGOH 4116
Affinity Capture-MS Homo sapiens
46 Ube2i  
Two-hybrid Mus musculus
47 MAGEB2 4113
Affinity Capture-MS Homo sapiens
48 SRPK1 6732
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
49 ST13 6767
Two-hybrid Homo sapiens
50 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ZBTB2 57621
Affinity Capture-MS Homo sapiens
52 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 DOCK8 81704
Affinity Capture-MS Homo sapiens
54 RPL15 6138
Affinity Capture-MS Homo sapiens
55 ECT2 1894
Affinity Capture-MS Homo sapiens
56 SRSF4 6429
Affinity Capture-MS Homo sapiens
57 YWHAZ 7534
Affinity Capture-MS Homo sapiens
58 SRSF5 6430
Affinity Capture-MS Homo sapiens
59 ZC3H11A  
Affinity Capture-MS Homo sapiens
60 SUZ12  
Affinity Capture-MS Homo sapiens
61 NXF1 10482
Two-hybrid Homo sapiens
62 NXF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PRR3  
Affinity Capture-MS Homo sapiens
64 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RBM26 64062
Two-hybrid Homo sapiens
66 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PABPC5 140886
Affinity Capture-MS Homo sapiens
68 CCDC88A 55704
Affinity Capture-MS Homo sapiens
69 CLCN2  
Affinity Capture-MS Homo sapiens
70 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
71 CLK1  
Two-hybrid Homo sapiens
72 Tubg1 103733
Affinity Capture-MS Mus musculus
73 FOLR1 2348
Affinity Capture-MS Homo sapiens
74 ERH 2079
Affinity Capture-MS Homo sapiens
75 Pum2  
Two-hybrid Mus musculus
76 APOBEC3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SAP18 10284
Affinity Capture-MS Homo sapiens
78 SNRPA 6626
Two-hybrid Homo sapiens
79 THRAP3 9967
Affinity Capture-MS Homo sapiens
80 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
81 PUF60 22827
Affinity Capture-MS Homo sapiens
82 TRA2B 6434
Two-hybrid Homo sapiens
83 MKI67  
Affinity Capture-MS Homo sapiens
84 Srsf1 110809
Affinity Capture-MS Mus musculus
85 SNIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SRSF3 6428
Two-hybrid Homo sapiens
87 KRTAP12-2  
Two-hybrid Homo sapiens
88 SRSF7 6432
Affinity Capture-MS Homo sapiens
89 CIT 11113
Affinity Capture-MS Homo sapiens
90 PRPF6 24148
Two-hybrid Homo sapiens
91 Srsf3 20383
Two-hybrid Mus musculus
92 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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