Gene description for VPS13C
Gene name vacuolar protein sorting 13 homolog C (S. cerevisiae)
Gene symbol VPS13C
Other names/aliases -
Species Homo sapiens
 Database cross references - VPS13C
ExoCarta ExoCarta_54832
Vesiclepedia VP_54832
Entrez Gene 54832
HGNC 23594
MIM 608879
UniProt Q709C8  
 VPS13C identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for VPS13C
Biological Process
    protein targeting to vacuole GO:0006623 IBA
    lipid transport GO:0006869 IEA
    Golgi to endosome transport GO:0006895 TAS
    mitochondrion organization GO:0007005 IBA
    mitochondrion organization GO:0007005 IMP
    response to insulin GO:0032868 IEA
    protein retention in Golgi apparatus GO:0045053 IBA
    negative regulation of type 2 mitophagy GO:1905090 IMP
Subcellular Localization
    cytoplasm GO:0005737 TAS
    mitochondrial outer membrane GO:0005741 IDA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    late endosome GO:0005770 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 IDA
    late endosome membrane GO:0031902 IEA
    dense core granule membrane GO:0032127 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified VPS13C in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for VPS13C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SGTA 6449
Proximity Label-MS Homo sapiens
3 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
4 DVL2 1856
Affinity Capture-MS Homo sapiens
5 LGALS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MAP2K2 5605
Affinity Capture-MS Homo sapiens
7 RAB35 11021
Proximity Label-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 MYCN  
Affinity Capture-MS Homo sapiens
10 HOOK1  
Proximity Label-MS Homo sapiens
11 LINC01587  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LAMTOR1 55004
Proximity Label-MS Homo sapiens
14 UBXN4 23190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HMGCLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CDK20  
Affinity Capture-MS Homo sapiens
17 MARCKS 4082
Proximity Label-MS Homo sapiens
18 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ATG5 9474
Proximity Label-MS Homo sapiens
20 LAMP3  
Proximity Label-MS Homo sapiens
21 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 BAG2 9532
Affinity Capture-MS Homo sapiens
23 ECT2 1894
Affinity Capture-MS Homo sapiens
24 PFN1 5216
Proximity Label-MS Homo sapiens
25 BAG5 9529
Affinity Capture-MS Homo sapiens
26 RAB11A 8766
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 IER3IP1 51124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 RAB1B 81876
Affinity Capture-MS Homo sapiens
29 RSAD2  
Affinity Capture-MS Homo sapiens
30 LMAN1 3998
Affinity Capture-MS Homo sapiens
31 GJD3  
Proximity Label-MS Homo sapiens
32 PRPH 5630
Proximity Label-MS Homo sapiens
33 LAMP2 3920
Proximity Label-MS Homo sapiens
34 RAB14 51552
Affinity Capture-MS Homo sapiens
35 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 GBA 2629
Affinity Capture-MS Homo sapiens
37 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
38 METTL21B  
Affinity Capture-MS Homo sapiens
39 MYC  
Affinity Capture-MS Homo sapiens
40 DUSP16  
Affinity Capture-MS Homo sapiens
41 RAB1A 5861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C10orf91  
Affinity Capture-MS Homo sapiens
43 TMEM55B 90809
Affinity Capture-MS Homo sapiens
44 RAB4A 5867
Proximity Label-MS Homo sapiens
45 FAM78B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RAB30 27314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 FGF14  
Affinity Capture-MS Homo sapiens
48 TMEM132D  
Two-hybrid Homo sapiens
49 RAB2A 5862
Proximity Label-MS Homo sapiens
50 ARL5B  
Affinity Capture-MS Homo sapiens
51 CALM2 805
Affinity Capture-MS Homo sapiens
52 RAB2B 84932
Affinity Capture-MS Homo sapiens
53 SOHLH1  
Affinity Capture-MS Homo sapiens
54 HIST1H2BA 255626
Cross-Linking-MS (XL-MS) Homo sapiens
55 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
56 RPS25 6230
Cross-Linking-MS (XL-MS) Homo sapiens
57 RAB9A 9367
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 CYB5R3 1727
Affinity Capture-MS Homo sapiens
59 RAB3B 5865
Proximity Label-MS Homo sapiens
60 UBTD1 80019
Affinity Capture-MS Homo sapiens
61 BANF1 8815
Affinity Capture-MS Homo sapiens
62 KRAS 3845
Proximity Label-MS Homo sapiens
63 RAB5C 5878
Proximity Label-MS Homo sapiens
64 CALM3 808
Affinity Capture-MS Homo sapiens
65 AR 367
Affinity Capture-MS Homo sapiens
66 GBA2  
Affinity Capture-MS Homo sapiens
67 ARPC5 10092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 COMMD5 28991
Affinity Capture-MS Homo sapiens
69 PLGRKT 55848
Affinity Capture-MS Homo sapiens
70 NUP210P1  
Affinity Capture-MS Homo sapiens
71 INF2 64423
Affinity Capture-MS Homo sapiens
72 RHOT2 89941
Proximity Label-MS Homo sapiens
73 C2CD2 25966
Affinity Capture-MS Homo sapiens
74 AKAP1 8165
Proximity Label-MS Homo sapiens
75 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 TMEM31  
Affinity Capture-MS Homo sapiens
77 SLC25A46 91137
Proximity Label-MS Homo sapiens
78 RAB32 10981
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which VPS13C is involved
No pathways found





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