Gene description for USP9Y
Gene name ubiquitin specific peptidase 9, Y-linked
Gene symbol USP9Y
Other names/aliases DFFRY
SPGFY2
Species Homo sapiens
 Database cross references - USP9Y
ExoCarta ExoCarta_8287
Vesiclepedia VP_8287
Entrez Gene 8287
HGNC 12633
MIM 400005
UniProt O00507  
 USP9Y identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for USP9Y
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type peptidase activity GO:0008234 TAS
    metal ion binding GO:0046872 IEA
    co-SMAD binding GO:0070410 ISS
Biological Process
    proteolysis GO:0006508 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 ISS
    spermatogenesis GO:0007283 TAS
    cell migration GO:0016477 IBA
    protein deubiquitination GO:0016579 ISS
    BMP signaling pathway GO:0030509 ISS
    regulation of protein stability GO:0031647 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IBA
 Experiment description of studies that identified USP9Y in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for USP9Y
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CES1P1  
Affinity Capture-MS Homo sapiens
2 MECP2 4204
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 ARHGEF2 9181
Cross-Linking-MS (XL-MS) Homo sapiens
5 SHC1 6464
Affinity Capture-MS Homo sapiens
6 PMS1  
Affinity Capture-Western Homo sapiens
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 CLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ECT2 1894
Affinity Capture-MS Homo sapiens
10 ZEB1  
Affinity Capture-MS Homo sapiens
11 DDHD1 80821
Affinity Capture-MS Homo sapiens
12 HS2ST1 9653
Affinity Capture-MS Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 SOCS1  
Affinity Capture-MS Homo sapiens
15 UBC 7316
Protein-peptide Homo sapiens
16 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DCLRE1C  
Affinity Capture-MS Homo sapiens
18 CCDC9  
Affinity Capture-MS Homo sapiens
19 KANSL3  
Affinity Capture-MS Homo sapiens
20 SIRT1  
Affinity Capture-MS Homo sapiens
21 TAGLN 6876
Affinity Capture-MS Homo sapiens
22 FAM103A1  
Affinity Capture-MS Homo sapiens
23 Hsph1 15505
Affinity Capture-MS Mus musculus
24 TMEM75  
Affinity Capture-MS Homo sapiens
25 EIF4B 1975
Affinity Capture-MS Homo sapiens
26 KIF23 9493
Affinity Capture-MS Homo sapiens
27 RPS6 6194
Cross-Linking-MS (XL-MS) Homo sapiens
28 AKT2 208
Affinity Capture-MS Homo sapiens
29 PYCR1 5831
Affinity Capture-MS Homo sapiens
30 VSX1  
Affinity Capture-MS Homo sapiens
31 CSNK1E 1454
Affinity Capture-MS Homo sapiens
32 ZNF331  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 VCPIP1 80124
Affinity Capture-MS Homo sapiens
34 KIAA0408  
Affinity Capture-MS Homo sapiens
35 CCL16  
Affinity Capture-MS Homo sapiens
36 C4orf47  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which USP9Y is involved
No pathways found





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