Gene description for MCM2
Gene name minichromosome maintenance complex component 2
Gene symbol MCM2
Other names/aliases BM28
CCNL1
CDCL1
D3S3194
MITOTIN
cdc19
Species Homo sapiens
 Database cross references - MCM2
ExoCarta ExoCarta_4171
Entrez Gene 4171
HGNC 6944
MIM 116945
UniProt P49736  
 MCM2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MCM2
Molecular Function
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IEA
    DNA replication origin binding GO:0003688 IEA
    DNA helicase activity GO:0003678 IEA
    DNA binding GO:0003677 TAS
Biological Process
    mitotic cell cycle GO:0000278 TAS
    cell cycle GO:0007049 TAS
    DNA unwinding involved in DNA replication GO:0006268 IEA
    DNA replication initiation GO:0006270 IMP
    cellular response to interleukin-4 GO:0071353 IEA
    nucleosome assembly GO:0006334 IEA
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    DNA replication GO:0006260 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    microtubule cytoskeleton GO:0015630 IDA
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    MCM complex GO:0042555 IDA
    nucleoplasm GO:0005654 TAS
    nuclear origin of replication recognition complex GO:0005664 IEA
 Experiment description of studies that identified MCM2 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Biochemical Activity Homo sapiens
2 ASK  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 MCM3 4172
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
4 ORC1L  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 CPSF1 29894
Invitro Homo sapiens
6 ORC2L  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 MCM5 4174
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 RPA2 6118
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 MCM7 4176
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
10 RPA1 6117
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 RPS4X 6191
Two-hybrid Homo sapiens
12 ORC5L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 MCM4 4173
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 CDK6 1021
Affinity Capture-MS Homo sapiens
15 PREI3  
Two-hybrid Homo sapiens
16 GTF2B 2959
Invitro Homo sapiens
17 HIST3H3 8290
Invitro Homo sapiens
Invivo Homo sapiens
18 MYST2  
Affinity Capture-MS Homo sapiens
19 GNB5 10681
Two-hybrid Homo sapiens
20 RPA3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
21 TBP  
Invitro Homo sapiens
22 AKAP8  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 MCM10 55388
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
24 GTF2H1 2965
Invitro Homo sapiens
25 ATM  
Biochemical Activity Homo sapiens
26 CDK4 1019
Affinity Capture-MS Homo sapiens
27 MCM2 4171
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 ORC4L  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
29 PHC2  
Two-hybrid Homo sapiens
30 CDC7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 MCM6 4175
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 MPP3  
Two-hybrid Homo sapiens
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