Gene description for SEC24B
Gene name SEC24 family member B
Gene symbol SEC24B
Other names/aliases SEC24
Species Homo sapiens
 Database cross references - SEC24B
ExoCarta ExoCarta_10427
Vesiclepedia VP_10427
Entrez Gene 10427
HGNC 10704
MIM 607184
UniProt O95487  
 SEC24B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for SEC24B
Molecular Function
    SNARE binding GO:0000149 IBA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
Biological Process
    neural tube closure GO:0001843 IEA
    outflow tract morphogenesis GO:0003151 IEA
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    cochlear nucleus development GO:0021747 IEA
    aorta morphogenesis GO:0035909 IEA
    auditory receptor cell stereocilium organization GO:0060088 IEA
    lung lobe morphogenesis GO:0060463 IEA
    coronary artery morphogenesis GO:0060982 IEA
    pulmonary artery morphogenesis GO:0061156 IEA
    COPII-coated vesicle cargo loading GO:0090110 IBA
    COPII-coated vesicle cargo loading GO:0090110 IDA
    regulation of establishment of planar polarity involved in neural tube closure GO:0090178 IEA
    regulation of cargo loading into COPII-coated vesicle GO:1901301 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    COPII vesicle coat GO:0030127 IBA
    COPII vesicle coat GO:0030127 IDA
    endoplasmic reticulum exit site GO:0070971 IBA
 Experiment description of studies that identified SEC24B in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SEC24B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 PLEKHA3  
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 OCLN 100506658
Proximity Label-MS Homo sapiens
5 AAK1 22848
Affinity Capture-MS Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 PPIE 10450
Affinity Capture-MS Homo sapiens
9 ISCA1  
Affinity Capture-MS Homo sapiens
10 TSG101 7251
Affinity Capture-MS Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 DVL3 1857
Affinity Capture-MS Homo sapiens
13 PTPN1 5770
Proximity Label-MS Homo sapiens
14 Actb 11461
Affinity Capture-MS Mus musculus
15 STX4 6810
Proximity Label-MS Homo sapiens
16 CENPA  
Proximity Label-MS Homo sapiens
17 TRPC5  
Affinity Capture-MS Homo sapiens
18 KCNJ10  
Affinity Capture-MS Homo sapiens
19 MCM2 4171
Affinity Capture-MS Homo sapiens
20 RAB3B 5865
Proximity Label-MS Homo sapiens
21 CAPZA1 829
Co-fractionation Homo sapiens
22 SSR1 6745
Proximity Label-MS Homo sapiens
23 DDOST 1650
Affinity Capture-MS Homo sapiens
24 STX6 10228
Proximity Label-MS Homo sapiens
25 SLC25A46 91137
Proximity Label-MS Homo sapiens
26 GTSE1 51512
Affinity Capture-MS Homo sapiens
27 SEC23A 10484
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
28 SNX27 81609
Affinity Capture-MS Homo sapiens
29 EBAG9 9166
Proximity Label-MS Homo sapiens
30 TMEM255A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 INSIG2  
Affinity Capture-MS Homo sapiens
32 RAB4A 5867
Proximity Label-MS Homo sapiens
33 RAB5A 5868
Proximity Label-MS Homo sapiens
34 RAD23B 5887
Co-fractionation Homo sapiens
35 GJA1 2697
Proximity Label-MS Homo sapiens
36 EMD 2010
Proximity Label-MS Homo sapiens
37 LUZP1 7798
Affinity Capture-MS Homo sapiens
38 SRA1 10011
Affinity Capture-MS Homo sapiens
39 CXADR 1525
Proximity Label-MS Homo sapiens
40 SCAP  
Reconstituted Complex Homo sapiens
41 Sec24c  
Affinity Capture-MS Mus musculus
42 PPIP5K2 23262
Affinity Capture-MS Homo sapiens
43 GOLGA2 2801
Proximity Label-MS Homo sapiens
44 CDH1 999
Proximity Label-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 RLIM 51132
Affinity Capture-MS Homo sapiens
47 PRRC1 133619
Affinity Capture-MS Homo sapiens
48 RPN1 6184
Affinity Capture-MS Homo sapiens
49 EYA4 2070
Affinity Capture-MS Homo sapiens
50 SEC61B 10952
Proximity Label-MS Homo sapiens
51 KDF1  
Affinity Capture-MS Homo sapiens
52 RPN2 6185
Affinity Capture-MS Homo sapiens
53 XPO1 7514
Affinity Capture-MS Homo sapiens
54 PANX1 24145
Proximity Label-MS Homo sapiens
55 SEC13 6396
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
56 PSMD13 5719
Co-fractionation Homo sapiens
57 CLTA 1211
Affinity Capture-MS Homo sapiens
58 CTNNB1 1499
Affinity Capture-MS Homo sapiens
59 NAA15 80155
Co-fractionation Homo sapiens
60 NUP88 4927
Affinity Capture-MS Homo sapiens
61 HSD3B7 80270
Proximity Label-MS Homo sapiens
62 CUL3 8452
Affinity Capture-MS Homo sapiens
63 STIM1 6786
Proximity Label-MS Homo sapiens
64 YIPF5 81555
Affinity Capture-MS Homo sapiens
65 SEC24A 10802
Co-fractionation Homo sapiens
Negative Genetic Homo sapiens
66 ZNF777  
Affinity Capture-MS Homo sapiens
67 UBE2D2 7322
Affinity Capture-MS Homo sapiens
68 MYC  
Affinity Capture-MS Homo sapiens
69 Uso1 56041
Affinity Capture-MS Mus musculus
70 SEC31A 22872
Co-fractionation Homo sapiens
71 ATP2A1 487
Proximity Label-MS Homo sapiens
72 SEC16A 9919
Affinity Capture-MS Homo sapiens
73 RAB9A 9367
Proximity Label-MS Homo sapiens
74 BICD1 636
Proximity Label-MS Homo sapiens
75 C8G 733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CLTC 1213
Affinity Capture-MS Homo sapiens
77 CCDC88A 55704
Affinity Capture-MS Homo sapiens
78 OST4  
Affinity Capture-MS Homo sapiens
79 STAT4  
Proximity Label-MS Homo sapiens
80 RNF43  
Proximity Label-MS Homo sapiens
81 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
82 VBP1 7411
Co-fractionation Homo sapiens
83 TERF2  
Affinity Capture-MS Homo sapiens
84 DAB2 1601
Affinity Capture-MS Homo sapiens
85 SEC23IP 11196
Co-fractionation Homo sapiens
86 PLEC 5339
Affinity Capture-MS Homo sapiens
87 URGCP 55665
Affinity Capture-MS Homo sapiens
88 SLC27A5 10998
Affinity Capture-MS Homo sapiens
89 SEC23B 10483
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 SQSTM1 8878
Proximity Label-MS Homo sapiens
91 TRPC3  
Affinity Capture-MS Homo sapiens
92 FYCO1 79443
Affinity Capture-MS Homo sapiens
93 QSER1  
Affinity Capture-MS Homo sapiens
94 NUP155 9631
Proximity Label-MS Homo sapiens
95 HNRNPK 3190
Co-fractionation Homo sapiens
96 LATS1  
Proximity Label-MS Homo sapiens
97 ERGIC2 51290
Proximity Label-MS Homo sapiens
98 TRIM37  
Proximity Label-MS Homo sapiens
99 RNMTL1  
Affinity Capture-MS Homo sapiens
100 B3GAT1  
Proximity Label-MS Homo sapiens
101 RAB7A 7879
Proximity Label-MS Homo sapiens
102 RAB11A 8766
Proximity Label-MS Homo sapiens
103 PTPRK 5796
Proximity Label-MS Homo sapiens
104 STT3B 201595
Affinity Capture-MS Homo sapiens
105 TCEAL4  
Co-fractionation Homo sapiens
106 KLHL12  
Affinity Capture-MS Homo sapiens
107 CAV1 857
Proximity Label-MS Homo sapiens
108 KCNA3  
Affinity Capture-Western Homo sapiens
109 DERL1 79139
Proximity Label-MS Homo sapiens
110 RNF139  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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