Experiment description of studies that identified RPSAP58 in exosomes

1 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

4 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

6 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

7 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for RPSAP58

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GNL2 29889 Affinity Capture-MS Homo sapiens 2 RPSA 3921 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 TADA1   Affinity Capture-MS Homo sapiens 4 CCSER1   Affinity Capture-MS Homo sapiens 5 CRBN   Affinity Capture-MS Homo sapiens 6 RPL26L1 51121 Affinity Capture-MS Homo sapiens 7 PPAN-P2RY11   Affinity Capture-MS Homo sapiens 8 ZBTB11   Affinity Capture-MS Homo sapiens 9 AATF   Affinity Capture-MS Homo sapiens 10 RPS3A 6189 Affinity Capture-MS Homo sapiens 11 NOL12 79159 Affinity Capture-MS Homo sapiens 12 RPS17 6218 Affinity Capture-MS Homo sapiens 13 SRPK2 6733 Affinity Capture-MS Homo sapiens 14 NOP14 8602 Affinity Capture-MS Homo sapiens 15 EPB41L5 57669 Affinity Capture-MS Homo sapiens 16 LARP1B 55132 Affinity Capture-MS Homo sapiens 17 ESF1 51575 Affinity Capture-MS Homo sapiens 18 BYSL 705 Affinity Capture-MS Homo sapiens 19 ZFR 51663 Affinity Capture-MS Homo sapiens 20 SSB 6741 Affinity Capture-MS Homo sapiens 21 DHX57 90957 Affinity Capture-MS Homo sapiens 22 RSBN1   Affinity Capture-MS Homo sapiens 23 RRP12 23223 Affinity Capture-MS Homo sapiens 24 UTP3 57050 Affinity Capture-MS Homo sapiens 25 FAU 2197 Affinity Capture-MS Homo sapiens 26 RPS3 6188 Affinity Capture-MS Homo sapiens 27 RPS18 6222 Affinity Capture-MS Homo sapiens 28 GPATCH4 54865 Affinity Capture-MS Homo sapiens 29 CDK14 5218 Affinity Capture-MS Homo sapiens 30 MCM2 4171 Affinity Capture-MS Homo sapiens 31 FAM170A   Affinity Capture-MS Homo sapiens 32 SF3B1 23451 Affinity Capture-MS Homo sapiens 33 CCDC137   Affinity Capture-MS Homo sapiens 34 IMP3 55272 Affinity Capture-MS Homo sapiens 35 MPHOSPH10 10199 Affinity Capture-MS Homo sapiens 36 RIOK2 55781 Affinity Capture-MS Homo sapiens 37 KRI1   Affinity Capture-MS Homo sapiens 38 RPS9 6203 Affinity Capture-MS Homo sapiens 39 SRP14 6727 Affinity Capture-MS Homo sapiens 40 STAU2 27067 Affinity Capture-MS Homo sapiens 41 MRPS7 51081 Affinity Capture-MS Homo sapiens 42 GLTSCR2   Affinity Capture-MS Homo sapiens 43 CDC5L 988 Affinity Capture-MS Homo sapiens 44 COMMD4 54939 Affinity Capture-MS Homo sapiens 45 DHX36 170506 Affinity Capture-MS Homo sapiens 46 RBM19 9904 Affinity Capture-MS Homo sapiens 47 GAR1 54433 Affinity Capture-MS Homo sapiens 48 ZCRB1   Affinity Capture-MS Homo sapiens 49 PARPBP   Affinity Capture-MS Homo sapiens 50 PPM1B 5495 Affinity Capture-MS Homo sapiens 51 HIST2H2AB 317772 Affinity Capture-MS Homo sapiens 52 YTHDF1 54915 Affinity Capture-MS Homo sapiens 53 DDX21 9188 Affinity Capture-MS Homo sapiens 54 RPL26 6154 Affinity Capture-MS Homo sapiens 55 RPS10 6204 Affinity Capture-MS Homo sapiens 56 CDR2L   Affinity Capture-MS Homo sapiens 57 HEY2   Affinity Capture-MS Homo sapiens 58 ZNF22   Affinity Capture-MS Homo sapiens 59 DDX10   Affinity Capture-MS Homo sapiens 60 RPL31 6160 Affinity Capture-MS Homo sapiens 61 CCNL1   Affinity Capture-MS Homo sapiens 62 RPS24 6229 Affinity Capture-MS Homo sapiens 63 CHERP 10523 Affinity Capture-MS Homo sapiens 64 FIGF 2277 Affinity Capture-MS Homo sapiens 65 ZFP91 80829 Affinity Capture-MS Homo sapiens 66 LYAR 55646 Affinity Capture-MS Homo sapiens 67 RPS6 6194 Affinity Capture-MS Homo sapiens 68 RPS15A 6210 Affinity Capture-MS Homo sapiens 69 DDX27 55661 Affinity Capture-MS Homo sapiens 70 GTPBP4 23560 Affinity Capture-MS Homo sapiens 71 METTL1 4234 Affinity Capture-MS Homo sapiens 72 LARP1 23367 Affinity Capture-MS Homo sapiens 73 RSL1D1 26156 Affinity Capture-MS Homo sapiens 74 CCDC59   Affinity Capture-MS Homo sapiens 75 OR6M1   Affinity Capture-MS Homo sapiens 76 YBX3 8531 Affinity Capture-MS Homo sapiens 77 BMS1   Affinity Capture-MS Homo sapiens 78 ABT1 29777 Affinity Capture-MS Homo sapiens 79 RPS2 6187 Affinity Capture-MS Homo sapiens 80 RPS19 6223 Affinity Capture-MS Homo sapiens 81 ZBTB24   Affinity Capture-MS Homo sapiens 82 NAT10 55226 Affinity Capture-MS Homo sapiens 83 RPS13 6207 Affinity Capture-MS Homo sapiens 84 SLC25A47   Affinity Capture-MS Homo sapiens 85 TAS2R50   Affinity Capture-MS Homo sapiens 86 HYPK 25764 Affinity Capture-MS Homo sapiens 87 CTCF   Affinity Capture-MS Homo sapiens 88 SGOL1   Affinity Capture-MS Homo sapiens 89 SRSF5 6430 Affinity Capture-MS Homo sapiens 90 PGAM4 441531 Affinity Capture-MS Homo sapiens 91 ZNF512   Affinity Capture-MS Homo sapiens 92 DKC1 1736 Affinity Capture-MS Homo sapiens 93 RNF212   Affinity Capture-MS Homo sapiens 94 VPS37D 155382 Affinity Capture-MS Homo sapiens 95 RPS4Y1 6192 Affinity Capture-MS Homo sapiens 96 RPS14 6208 Affinity Capture-MS Homo sapiens 97 GZF1   Affinity Capture-MS Homo sapiens 98 SPAST 6683 Affinity Capture-MS Homo sapiens 99 NOP16 51491 Affinity Capture-MS Homo sapiens 100 TSR1 55720 Affinity Capture-MS Homo sapiens 101 SREK1 140890 Affinity Capture-MS Homo sapiens 102 NOL6 65083 Affinity Capture-MS Homo sapiens 103 SF3B2 10992 Affinity Capture-MS Homo sapiens 104 KIAA1522 57648 Affinity Capture-MS Homo sapiens 105 NGDN   Affinity Capture-MS Homo sapiens 106 RPS16 6217 Affinity Capture-MS Homo sapiens 107 U2SURP 23350 Affinity Capture-MS Homo sapiens 108 ZNF574   Affinity Capture-MS Homo sapiens 109 ST3GAL5   Affinity Capture-MS Homo sapiens 110 YBX1 4904 Affinity Capture-MS Homo sapiens 111 SURF6   Affinity Capture-MS Homo sapiens 112 SLA2 84174 Affinity Capture-MS Homo sapiens 113 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 114 CCDC68   Affinity Capture-MS Homo sapiens 115 EPB41L4B 54566 Affinity Capture-MS Homo sapiens 116 SRRM1 10250 Affinity Capture-MS Homo sapiens 117 SDR16C5   Affinity Capture-MS Homo sapiens 118 RPS21 6227 Affinity Capture-MS Homo sapiens 119 NOL10   Affinity Capture-MS Homo sapiens 120 PRRC2C 23215 Affinity Capture-MS Homo sapiens 121 KNOP1 400506 Affinity Capture-MS Homo sapiens 122 RPS4X 6191 Affinity Capture-MS Homo sapiens 123 RPS15 6209 Affinity Capture-MS Homo sapiens 124 C1orf35   Affinity Capture-MS Homo sapiens 125 TRAPPC13 80006 Affinity Capture-MS Homo sapiens 126 CNOT3 4849 Affinity Capture-MS Homo sapiens 127 RPS5 6193 Affinity Capture-MS Homo sapiens 128 NIFK 84365 Affinity Capture-MS Homo sapiens 129 TMEM154   Affinity Capture-MS Homo sapiens 130 TRIP12 9320 Affinity Capture-MS Homo sapiens 131 KRR1 11103 Affinity Capture-MS Homo sapiens 132 LARP7 51574 Affinity Capture-MS Homo sapiens 133 RPS4Y2 140032 Affinity Capture-MS Homo sapiens 134 OR5M11   Affinity Capture-MS Homo sapiens 135 EIF4B 1975 Affinity Capture-MS Homo sapiens 136 PRKRIR   Affinity Capture-MS Homo sapiens 137 ZNF768 79724 Affinity Capture-MS Homo sapiens 138 RPS8 6202 Affinity Capture-MS Homo sapiens 139 CDR2   Affinity Capture-MS Homo sapiens 140 SERBP1 26135 Affinity Capture-MS Homo sapiens 141 LTV1   Affinity Capture-MS Homo sapiens 142 POLR1D 51082 Affinity Capture-MS Homo sapiens 143 RPL36AL 6166 Affinity Capture-MS Homo sapiens 144 PURA 5813 Affinity Capture-MS Homo sapiens 145 ZSCAN25   Affinity Capture-MS Homo sapiens 146 ZNF770 54989 Affinity Capture-MS Homo sapiens 147 IMP4   Affinity Capture-MS Homo sapiens 148 UTP23   Affinity Capture-MS Homo sapiens View the network

Pathways in which RPSAP58 is involved

No pathways found