Gene description for PHF5A
Gene name PHD finger protein 5A
Gene symbol PHF5A
Other names/aliases INI
Rds3
SAP14b
SF3B7
SF3b14b
bK223H9.2
Species Homo sapiens
 Database cross references - PHF5A
ExoCarta ExoCarta_84844
Entrez Gene 84844
HGNC 18000
UniProt Q7RTV0  
 PHF5A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PHF5A
Molecular Function
    DNA binding GO:0003677 IEA
    poly(A) RNA binding GO:0044822 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 ISS
Biological Process
    RNA splicing GO:0008380 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    gene expression GO:0010467 TAS
Subcellular Localization
    U12-type spliceosomal complex GO:0005689 IDA
    nucleoplasm GO:0005654 TAS
    U2 snRNP GO:0005686 IDA
    nuclear matrix GO:0016363 IEA
    nuclear speck GO:0016607 ISS
 Experiment description of studies that identified PHF5A in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHF5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX42  
Invivo Homo sapiens
2 SF3B5  
Invivo Homo sapiens
3 RNU11 26824
Affinity Capture-MS Homo sapiens
4 SF3B6 51639
Invivo Homo sapiens
5 SF3B3 23450
Invivo Homo sapiens
6 RNU12  
Affinity Capture-MS Homo sapiens
7 SF3B2 10992
Invivo Homo sapiens
8 SF3B4 10262
Invivo Homo sapiens
9 SF3B1 23451
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PHF5A is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome





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