Gene description for PRDX3
Gene name peroxiredoxin 3
Gene symbol PRDX3
Other names/aliases AOP-1
AOP1
HBC189
MER5
PRO1748
SP-22
prx-III
Species Homo sapiens
 Database cross references - PRDX3
ExoCarta ExoCarta_10935
Entrez Gene 10935
HGNC 9354
MIM 604769
UniProt P30048  
 PRDX3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Hepatocytes 26054723    
 Gene ontology annotations for PRDX3
Molecular Function
    protein kinase binding GO:0019901 IPI
    alkyl hydroperoxide reductase activity GO:0008785 NAS
    identical protein binding GO:0042802 IEA
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    thioredoxin peroxidase activity GO:0008379 IDA
    protein C-terminus binding GO:0008022 IPI
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IMP
Biological Process
    cellular response to oxidative stress GO:0034599 IDA
    peptidyl-cysteine oxidation GO:0018171 IDA
    maternal placenta development GO:0001893 IEA
    hydrogen peroxide catabolic process GO:0042744 IMP
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IMP
    response to hydrogen peroxide GO:0042542 IDA
    response to oxidative stress GO:0006979 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of kinase activity GO:0033673 IDA
    response to reactive oxygen species GO:0000302 TAS
    apoptotic process GO:0006915 IMP
    positive regulation of cell proliferation GO:0008284 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    response to lipopolysaccharide GO:0032496 ISS
    regulation of mitochondrial membrane potential GO:0051881 IMP
    cellular response to reactive oxygen species GO:0034614 IMP
    myeloid cell differentiation GO:0030099 ISS
    mitochondrion organization GO:0007005 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    extracellular exosome GO:0070062 IDA
    IkappaB kinase complex GO:0008385 IPI
    mitochondrial matrix GO:0005759 TAS
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 IDA
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified PRDX3 in exosomes
1
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PRDX3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP3K13 9175
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 IKBKB 3551
Affinity Capture-Western Homo sapiens
3 MAGEA11  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PRDX3 is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species TAS Reactome





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