Gene ontology annotations for NPM3 |
|
Experiment description of studies that identified NPM3 in exosomes |
1 |
Experiment ID |
412 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MCF7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
2 |
Experiment ID |
414 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
3 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
4 |
Experiment ID |
427 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
5 |
Experiment ID |
407 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
6 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
7 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
8 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
9 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
405 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Foreskin fibroblasts |
Sample name |
BJ |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
11 |
Experiment ID |
417 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Lymphoma cells |
Sample name |
Raji |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
12 |
Experiment ID |
411 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Mammary cancer-associated fibroblasts |
Sample name |
mCAF |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
13 |
Experiment ID |
418 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Monocytic leukemia cells |
Sample name |
THP-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
14 |
Experiment ID |
418 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Monocytic leukemia cells |
Sample name |
THP-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
15 |
Experiment ID |
413 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Normal mammary epithelial cells |
Sample name |
MCF10A |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
16 |
Experiment ID |
406 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
BxPC3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
17 |
Experiment ID |
415 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
18 |
Experiment ID |
434 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
19 |
Experiment ID |
435 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
20 |
Experiment ID |
408 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic duct epithalial cells |
Sample name |
HPDE |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
21 |
Experiment ID |
409 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic duct epithalial cells |
Sample name |
HPNE |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
22 |
Experiment ID |
416 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pluripotent stem cells |
Sample name |
PSC |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
23 |
Experiment ID |
410 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
T lymphocytes |
Sample name |
Jurkat |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
24 |
Experiment ID |
217 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|CD81|CD9|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
Protein-protein interactions for NPM3 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
1 |
GNL2 |
29889 |
Affinity Capture-MS |
 |
Homo sapiens |
|
2 |
UBE2H |
7328 |
Affinity Capture-MS |
 |
Homo sapiens |
|
3 |
RPP14 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
4 |
ZNF668 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
5 |
SSR4 |
6748 |
Affinity Capture-MS |
 |
Homo sapiens |
|
6 |
SENP3 |
26168 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
7 |
RPL26L1 |
51121 |
Affinity Capture-MS |
 |
Homo sapiens |
|
8 |
ZNF16 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
9 |
KPNA3 |
3839 |
Affinity Capture-MS |
 |
Homo sapiens |
|
10 |
DDX24 |
57062 |
Affinity Capture-MS |
 |
Homo sapiens |
|
11 |
AATF |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
12 |
EEF1G |
1937 |
Co-fractionation |
 |
Homo sapiens |
|
13 |
RPS3A |
6189 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
14 |
MRPS18B |
28973 |
Affinity Capture-MS |
 |
Homo sapiens |
|
15 |
KIAA0020 |
9933 |
Affinity Capture-MS |
 |
Homo sapiens |
|
16 |
RSL24D1 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
17 |
TPD52L1 |
7164 |
Co-fractionation |
 |
Homo sapiens |
|
18 |
YWHAE |
7531 |
Co-fractionation |
 |
Homo sapiens |
|
19 |
RPL13A |
23521 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
20 |
RPL37A |
6168 |
Affinity Capture-MS |
 |
Homo sapiens |
|
21 |
URB1 |
9875 |
Affinity Capture-MS |
 |
Homo sapiens |
|
22 |
EPB41L5 |
57669 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
23 |
NMNAT1 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
24 |
RPL10 |
6134 |
Affinity Capture-MS |
 |
Homo sapiens |
|
25 |
RPL15 |
6138 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
26 |
HSP90AB1 |
3326 |
Co-fractionation |
 |
Homo sapiens |
|
27 |
MRPS17 |
51373 |
Affinity Capture-MS |
 |
Homo sapiens |
|
28 |
PRPF40A |
55660 |
Co-fractionation |
 |
Homo sapiens |
|
29 |
MRPS27 |
23107 |
Affinity Capture-MS |
 |
Homo sapiens |
|
30 |
WDR36 |
134430 |
Affinity Capture-MS |
 |
Homo sapiens |
|
31 |
USP36 |
|
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
32 |
ZNF70 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
33 |
NPM2 |
|
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Two-hybrid |
 |
Homo sapiens |
|
34 |
NTN1 |
9423 |
Affinity Capture-MS |
 |
Homo sapiens |
|
35 |
RPL10A |
4736 |
Affinity Capture-MS |
 |
Homo sapiens |
|
36 |
MRPL20 |
55052 |
Affinity Capture-MS |
 |
Homo sapiens |
|
37 |
WHSC1 |
7468 |
Affinity Capture-MS |
 |
Homo sapiens |
|
38 |
MRPS23 |
51649 |
Affinity Capture-MS |
 |
Homo sapiens |
|
39 |
MCM2 |
4171 |
Affinity Capture-MS |
 |
Homo sapiens |
|
40 |
WT1 |
|
Two-hybrid |
 |
Homo sapiens |
|
41 |
WDR74 |
54663 |
Affinity Capture-MS |
 |
Homo sapiens |
|
42 |
CCDC137 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
43 |
IMP3 |
55272 |
Affinity Capture-MS |
 |
Homo sapiens |
|
44 |
MPHOSPH10 |
10199 |
Affinity Capture-MS |
 |
Homo sapiens |
|
45 |
AP1G1 |
164 |
Co-fractionation |
 |
Homo sapiens |
|
46 |
NPM1 |
4869 |
Affinity Capture-Western |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-Western |
 |
Homo sapiens |
Reconstituted Complex |
 |
Homo sapiens |
Co-fractionation |
 |
Homo sapiens |
Co-fractionation |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
47 |
HIST1H2AG |
8969 |
Affinity Capture-MS |
 |
Homo sapiens |
|
48 |
RPS8 |
6202 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
49 |
WDR12 |
55759 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
50 |
RECQL4 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
51 |
KRI1 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
52 |
RPS9 |
6203 |
Affinity Capture-MS |
 |
Homo sapiens |
|
53 |
MAGED2 |
10916 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
54 |
NSUN4 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
55 |
RPF2 |
84154 |
Affinity Capture-MS |
 |
Homo sapiens |
|
56 |
TTF1 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
57 |
PAK1IP1 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
58 |
PRKRA |
8575 |
Affinity Capture-MS |
 |
Homo sapiens |
|
59 |
DDX51 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
60 |
NIFK |
84365 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
61 |
EED |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
62 |
ABT1 |
29777 |
Affinity Capture-MS |
 |
Homo sapiens |
|
63 |
Naa11 |
|
Affinity Capture-MS |
 |
Mus musculus |
|
64 |
ATG16L1 |
55054 |
Affinity Capture-MS |
 |
Homo sapiens |
|
65 |
SENP5 |
|
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
66 |
RPL34 |
6164 |
Affinity Capture-MS |
 |
Homo sapiens |
|
67 |
Eif3a |
13669 |
Affinity Capture-MS |
 |
Mus musculus |
|
68 |
DNM1 |
1759 |
Co-fractionation |
 |
Homo sapiens |
|
69 |
MAK16 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
70 |
C18orf21 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
71 |
RPL19 |
6143 |
Affinity Capture-MS |
 |
Homo sapiens |
|
72 |
CTCF |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
73 |
PES1 |
23481 |
Affinity Capture-MS |
 |
Homo sapiens |
|
74 |
FAS |
355 |
Proximity Label-MS |
 |
Homo sapiens |
|
75 |
BRIX1 |
55299 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
76 |
RPL27 |
6155 |
Affinity Capture-MS |
 |
Homo sapiens |
|
77 |
DDX31 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
78 |
PNO1 |
56902 |
Affinity Capture-MS |
 |
Homo sapiens |
|
79 |
HIST2H2AB |
317772 |
Affinity Capture-MS |
 |
Homo sapiens |
|
80 |
HIST1H4A |
8359 |
Affinity Capture-MS |
 |
Homo sapiens |
|
81 |
DDX21 |
9188 |
Affinity Capture-MS |
 |
Homo sapiens |
|
82 |
RPL9 |
6133 |
Affinity Capture-MS |
 |
Homo sapiens |
|
83 |
RPL4 |
6124 |
Affinity Capture-MS |
 |
Homo sapiens |
|
84 |
RBM28 |
55131 |
Affinity Capture-MS |
 |
Homo sapiens |
|
85 |
CENPV |
201161 |
Affinity Capture-MS |
 |
Homo sapiens |
|
86 |
IRF2BPL |
64207 |
Co-fractionation |
 |
Homo sapiens |
|
87 |
XRN1 |
54464 |
Affinity Capture-MS |
 |
Homo sapiens |
|
88 |
STAU1 |
6780 |
Affinity Capture-MS |
 |
Homo sapiens |
|
89 |
NOP2 |
4839 |
Affinity Capture-MS |
 |
Homo sapiens |
|
90 |
ZNF22 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
91 |
RPL5 |
6125 |
Affinity Capture-MS |
 |
Homo sapiens |
|
92 |
NOL7 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
93 |
DDX10 |
|
| | | |