Gene description for CSTA
Gene name cystatin A (stefin A)
Gene symbol CSTA
Other names/aliases AREI
STF1
STFA
Species Homo sapiens
 Database cross references - CSTA
ExoCarta ExoCarta_1475
Entrez Gene 1475
HGNC 2481
MIM 184600
UniProt P01040  
 CSTA identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CSTA
Molecular Function
    cysteine-type endopeptidase inhibitor activity GO:0004869 IDA
    structural molecule activity GO:0005198 IDA
    protease binding GO:0002020 IPI
    protein binding, bridging GO:0030674 IDA
Biological Process
    negative regulation of endopeptidase activity GO:0010951 IEA
    negative regulation of proteolysis GO:0045861 IDA
    keratinocyte differentiation GO:0030216 IDA
    peptide cross-linking GO:0018149 IDA
    single organismal cell-cell adhesion GO:0016337 IMP
    negative regulation of peptidase activity GO:0010466 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cornified envelope GO:0001533 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified CSTA in exosomes
1
Experiment ID 266
ISEV standards
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 267
ISEV standards
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 268
ISEV standards
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSTA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PI3  
Reconstituted Complex Homo sapiens
2 CTSB 1508
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 CTSL 1514
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 IVL 3713
Reconstituted Complex Homo sapiens
5 KRT1 3848
Reconstituted Complex Homo sapiens
6 CSTA 1475
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
7 LOR  
Reconstituted Complex Homo sapiens
8 DSP 1832
Reconstituted Complex Homo sapiens
9 CTSH 1512
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml
 Pathways in which CSTA is involved
No pathways found





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