Gene description for SERPINB9
Gene name serpin peptidase inhibitor, clade B (ovalbumin), member 9
Gene symbol SERPINB9
Other names/aliases CAP-3
CAP3
PI-9
PI9
Species Homo sapiens
 Database cross references - SERPINB9
ExoCarta ExoCarta_5272
Entrez Gene 5272
HGNC 8955
MIM 601799
UniProt P50453  
 SERPINB9 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Endothelial cells 26027894    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SERPINB9
Molecular Function
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IMP
    serine-type endopeptidase inhibitor activity GO:0004867 IDA
    protease binding GO:0002020 IPI
    protein binding GO:0005515 IPI
Biological Process
    response to bacterium GO:0009617 IDA
    negative regulation of endopeptidase activity GO:0010951 IMP
    mast cell mediated immunity GO:0002448 IEP
    apoptotic process GO:0006915 IMP
    protection from natural killer cell mediated cytotoxicity GO:0042270 IMP
    cellular response to estrogen stimulus GO:0071391 IEP
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IMP
    negative regulation by symbiont of host apoptotic process GO:0033668 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    immune response GO:0006955 IDA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    intracellular GO:0005622 IDA
    membrane GO:0016020 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified SERPINB9 in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SERPINB9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GBP2  
Two-hybrid Homo sapiens
2 CASP4  
Invivo Homo sapiens
3 TUBB2A 7280
Two-hybrid Homo sapiens
4 BRD7  
Two-hybrid Homo sapiens
5 ECH1 1891
Two-hybrid Homo sapiens
6 MGC15730  
Two-hybrid Homo sapiens
7 TLE1  
Two-hybrid Homo sapiens
8 EEF1A1 1915
Two-hybrid Homo sapiens
9 C14orf1 11161
Two-hybrid Homo sapiens
10 ZHX1  
Two-hybrid Homo sapiens
11 RIF1  
Two-hybrid Homo sapiens
12 DKFZP564O0523  
Two-hybrid Homo sapiens
13 COPS6 10980
Two-hybrid Homo sapiens
14 GDF9 2661
Two-hybrid Homo sapiens
15 UNC119  
Two-hybrid Homo sapiens
16 PLEKHM1  
Two-hybrid Homo sapiens
17 SETDB1  
Two-hybrid Homo sapiens
18 GZMB  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
19 MDS025  
Two-hybrid Homo sapiens
20 GAPDH 2597
Two-hybrid Homo sapiens
21 XRCC6 2547
Two-hybrid Homo sapiens
22 UBR1  
Two-hybrid Homo sapiens
23 TP53  
Two-hybrid Homo sapiens
24 PRPF40A 55660
Two-hybrid Homo sapiens
25 MED31  
Two-hybrid Homo sapiens
26 CRMP1 1400
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SERPINB9 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here