Gene description for IKBKAP
Gene name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
Gene symbol IKBKAP
Other names/aliases DYS
ELP1
FD
IKAP
IKI3
TOT1
Species Homo sapiens
 Database cross references - IKBKAP
ExoCarta ExoCarta_8518
Entrez Gene 8518
HGNC 5959
MIM 603722
UniProt O95163  
 IKBKAP identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for IKBKAP
Molecular Function
    RNA polymerase II core binding GO:0000993 IDA
    phosphorylase kinase regulator activity GO:0008607 IDA
    signal transducer activity GO:0004871 TAS
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cell migration GO:0030335 ISS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    immune response GO:0006955 TAS
    regulation of protein kinase activity GO:0045859 TAS
    chromatin organization GO:0006325 TAS
    signal transduction GO:0007165 TAS
    protein complex assembly GO:0006461 TAS
    protein phosphorylation GO:0006468 TAS
Subcellular Localization
    Elongator holoenzyme complex GO:0033588 IDA
    cytoplasm GO:0005737 IDA
    transcription elongation factor complex GO:0008023 IDA
    nucleolus GO:0005730 IDA
    histone acetyltransferase complex GO:0000123 IDA
 Experiment description of studies that identified IKBKAP in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IKBKAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDHAF2 54949
Two-hybrid Homo sapiens
2 NIPSNAP3A  
Two-hybrid Homo sapiens
3 BAG6 7917
Two-hybrid Homo sapiens
4 TTR 7276
Two-hybrid Homo sapiens
5 PLP2 5355
Two-hybrid Homo sapiens
6 MAPK8 5599
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
7 ELP3 55140
Invivo Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
8 NDUFB9  
Two-hybrid Homo sapiens
9 CDKN2B 1030
Two-hybrid Homo sapiens
10 TAC3  
Two-hybrid Homo sapiens
11 SNAPIN  
Two-hybrid Homo sapiens
12 MRPL42  
Two-hybrid Homo sapiens
13 IKBKB 3551
Invivo Homo sapiens
Invitro Homo sapiens
14 MAN2A2  
Two-hybrid Homo sapiens
15 CHUK 1147
Invivo Homo sapiens
Invitro Homo sapiens
16 CCT5 22948
Two-hybrid Homo sapiens
17 CSTF2  
Two-hybrid Homo sapiens
18 TXNDC9  
Two-hybrid Homo sapiens
19 MAP3K14  
Invivo Homo sapiens
Invitro Homo sapiens
20 ELP4  
Co-purification Homo sapiens
21 ELP2 55250
Co-purification Homo sapiens
View the network image/svg+xml
 Pathways in which IKBKAP is involved
PathwayEvidenceSource
HATs acetylate histones TAS Reactome





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