Gene description for IKBKAP
Gene name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
Gene symbol IKBKAP
Other names/aliases DYS
ELP1
FD
IKAP
IKI3
TOT1
Species Homo sapiens
 Database cross references - IKBKAP
ExoCarta ExoCarta_8518
Vesiclepedia VP_8518
Entrez Gene 8518
HGNC 5959
MIM 603722
UniProt O95163  
 IKBKAP identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for IKBKAP
Molecular Function
    tRNA binding GO:0000049 IBA
    protein binding GO:0005515 IPI
Biological Process
    tRNA wobble uridine modification GO:0002098 NAS
    tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation GO:0002926 IBA
    regulation of translation GO:0006417 NAS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    elongator holoenzyme complex GO:0033588 IBA
    elongator holoenzyme complex GO:0033588 IDA
    elongator holoenzyme complex GO:0033588 IPI
 Experiment description of studies that identified IKBKAP in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1275
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1280
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IKBKAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 MPG 4350
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CLIP1 6249
Proximity Label-MS Homo sapiens
4 IRF4  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 SHC1 6464
Affinity Capture-MS Homo sapiens
6 ELP6 54859
Affinity Capture-Western Homo sapiens
7 PKM 5315
Affinity Capture-MS Homo sapiens
8 RIT1 6016
Negative Genetic Homo sapiens
9 PSMD11 5717
Co-fractionation Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 P4HA2 8974
Co-fractionation Homo sapiens
12 TTR 7276
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 CD2AP 23607
Co-fractionation Homo sapiens
14 TAC3  
Two-hybrid Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 USP9X 8239
Affinity Capture-MS Homo sapiens
17 ARCN1 372
Co-fractionation Homo sapiens
18 FAM219B 57184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PRKDC 5591
Affinity Capture-MS Homo sapiens
20 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
21 NDUFB9 4715
Two-hybrid Homo sapiens
22 MCM2 4171
Co-fractionation Homo sapiens
23 SF3B1 23451
Co-fractionation Homo sapiens
24 COPB2 9276
Co-fractionation Homo sapiens
25 FBXW7  
Affinity Capture-MS Homo sapiens
26 PSMD12 5718
Co-fractionation Homo sapiens
27 PPP6R1 22870
Proximity Label-MS Homo sapiens
28 MCM6 4175
Co-fractionation Homo sapiens
29 ELP4  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
30 ATG16L1 55054
Affinity Capture-MS Homo sapiens
31 DPH3  
Co-fractionation Homo sapiens
32 KPTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 PFN1 5216
Proximity Label-MS Homo sapiens
35 ZNF217 7764
Co-fractionation Homo sapiens
36 KRT8 3856
Proximity Label-MS Homo sapiens
37 CAMK2A 815
Affinity Capture-MS Homo sapiens
38 PPP5C 5536
Affinity Capture-MS Homo sapiens
39 FHL3 2275
Co-fractionation Homo sapiens
40 PSMD4 5710
Co-fractionation Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 DCTN5 84516
Co-fractionation Homo sapiens
43 MYLK2 85366
Affinity Capture-MS Homo sapiens
44 CSTF2 1478
Two-hybrid Homo sapiens
45 COPG1 22820
Co-fractionation Homo sapiens
46 PSMC1 5700
Co-fractionation Homo sapiens
47 CCT5 22948
Two-hybrid Homo sapiens
48 BAG6 7917
Two-hybrid Homo sapiens
49 HTT 3064
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
50 ACACA 31
Affinity Capture-MS Homo sapiens
51 MAN2A2 4122
Two-hybrid Homo sapiens
52 BARD1 580
Two-hybrid Homo sapiens
53 NTRK1 4914
Affinity Capture-MS Homo sapiens
54 BMX  
Affinity Capture-MS Homo sapiens
55 MAPK8 5599
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
56 DNAJB11 51726
Affinity Capture-MS Homo sapiens
57 C17orf53  
Proximity Label-MS Homo sapiens
58 KDF1  
Affinity Capture-MS Homo sapiens
59 SF3B2 10992
Co-fractionation Homo sapiens
60 FTL 2512
Affinity Capture-MS Homo sapiens
61 SIRT7  
Affinity Capture-MS Homo sapiens
62 EPAS1  
Affinity Capture-MS Homo sapiens
63 NFKBIA  
Reconstituted Complex Homo sapiens
64 PRDX1 5052
Affinity Capture-MS Homo sapiens
65 ELP3 55140
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
66 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 NR3C1 2908
Proximity Label-MS Homo sapiens
68 PSMA2 5683
Co-fractionation Homo sapiens
69 EGFR 1956
Affinity Capture-MS Homo sapiens
70 MCM4 4173
Co-fractionation Homo sapiens
71 SNX27 81609
Affinity Capture-MS Homo sapiens
72 ELP2 55250
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
73 TXNDC9 10190
Two-hybrid Homo sapiens
74 KDM3B  
Co-fractionation Homo sapiens
75 FKBP5 2289
Affinity Capture-MS Homo sapiens
76 MAPK6  
Affinity Capture-MS Homo sapiens
77 CDKN2B 1030
Two-hybrid Homo sapiens
78 RPA3 6119
Proximity Label-MS Homo sapiens
79 APEH 327
Co-fractionation Homo sapiens
80 FOCAD 54914
Co-fractionation Homo sapiens
81 PHF5A 84844
Co-fractionation Homo sapiens
82 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
83 PSMC5 5705
Affinity Capture-MS Homo sapiens
84 DUSP9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PSMB5 5693
Affinity Capture-MS Homo sapiens
86 PLP2 5355
Two-hybrid Homo sapiens
87 KRAS 3845
Negative Genetic Homo sapiens
88 HAX1  
Proximity Label-MS Homo sapiens
89 MAP3K7  
Affinity Capture-Western Homo sapiens
90 FSCN1 6624
Affinity Capture-MS Homo sapiens
91 CHUK 1147
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
92 PASK  
Affinity Capture-MS Homo sapiens
93 CCDC8  
Affinity Capture-MS Homo sapiens
94 HIF1A 3091
Affinity Capture-MS Homo sapiens
95 ZRANB1 54764
Affinity Capture-MS Homo sapiens
96 SDHAF2 54949
Two-hybrid Homo sapiens
97 NIPSNAP3A  
Two-hybrid Homo sapiens
98 RELA 5970
Co-fractionation Homo sapiens
99 IKBKAP 8518
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-crystal Structure Homo sapiens
100 MRPL42  
Two-hybrid Homo sapiens
101 MAP3K14  
Affinity Capture-Western Homo sapiens
102 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
103 PRKCSH 5589
Co-fractionation Homo sapiens
104 SIRT6  
Affinity Capture-MS Homo sapiens
105 NUP85 79902
Co-fractionation Homo sapiens
106 RPS15 6209
Cross-Linking-MS (XL-MS) Homo sapiens
107 PRKRIP1 79706
Co-fractionation Homo sapiens
108 SNAPIN 23557
Two-hybrid Homo sapiens
109 OTUB1 55611
Affinity Capture-MS Homo sapiens
110 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
111 NUP50 10762
Co-fractionation Homo sapiens
112 EEF1A1P5 158078
Cross-Linking-MS (XL-MS) Homo sapiens
113 TSC22D1 8848
Co-fractionation Homo sapiens
114 FLNA 2316
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
115 SSSCA1 10534
Affinity Capture-MS Homo sapiens
116 ELP5 23587
Affinity Capture-Western Homo sapiens
117 CAMK2D 817
Affinity Capture-MS Homo sapiens
118 HNRNPU 3192
Co-fractionation Homo sapiens
119 C9orf72  
Affinity Capture-MS Homo sapiens
120 MYC  
Affinity Capture-MS Homo sapiens
121 HSPA12A 259217
Proximity Label-MS Homo sapiens
122 PSMC4 5704
Co-fractionation Homo sapiens
123 IKBKB 3551
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
124 DCP2  
Affinity Capture-MS Homo sapiens
125 ACBD3 64746
Co-fractionation Homo sapiens
126 WDHD1  
Co-fractionation Homo sapiens
127 EP300 2033
Affinity Capture-MS Homo sapiens
128 CDC5L 988
Affinity Capture-MS Homo sapiens
129 PLEKHN1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which IKBKAP is involved
PathwayEvidenceSource
Chromatin modifying enzymes IEA Reactome
Chromatin organization IEA Reactome
HATs acetylate histones IEA Reactome





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