Gene description for NEFM
Gene name neurofilament, medium polypeptide
Gene symbol NEFM
Other names/aliases NEF3
NF-M
NFM
Species Homo sapiens
 Database cross references - NEFM
ExoCarta ExoCarta_4741
Vesiclepedia VP_4741
Entrez Gene 4741
HGNC 7734
MIM 162250
UniProt P07197  
 NEFM identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NEFM
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 TAS
Biological Process
    neurofilament bundle assembly GO:0033693 IBA
    neurofilament bundle assembly GO:0033693 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    intermediate filament GO:0005882 IBA
    neurofilament GO:0005883 TAS
    axon GO:0030424 IBA
    axon GO:0030424 TAS
    intermediate filament cytoskeleton GO:0045111 IDA
    neurofibrillary tangle GO:0097418 IDA
    postsynaptic intermediate filament cytoskeleton GO:0099160 IBA
 Experiment description of studies that identified NEFM in exosomes
1
Experiment ID 267
MISEV standards
Biophysical techniques
TSG101|Alix
Enriched markers
CANX|HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NEFM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Affinity Capture-MS Homo sapiens
2 SLC25A13 10165
Proximity Label-MS Homo sapiens
3 KRT37 8688
Affinity Capture-MS Homo sapiens
4 DLG5 9231
Proximity Label-MS Homo sapiens
5 SENP3 26168
Proximity Label-MS Homo sapiens
6 TPGS1  
Proximity Label-MS Homo sapiens
7 SETBP1  
Cross-Linking-MS (XL-MS) Homo sapiens
8 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SYCE1  
Affinity Capture-MS Homo sapiens
10 NUP188 23511
Proximity Label-MS Homo sapiens
11 DNTTIP1 116092
Affinity Capture-MS Homo sapiens
12 NUP62 23636
Affinity Capture-MS Homo sapiens
13 FASTKD5  
Proximity Label-MS Homo sapiens
14 GFAP 2670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 WARS 7453
Cross-Linking-MS (XL-MS) Homo sapiens
17 SSB 6741
Cross-Linking-MS (XL-MS) Homo sapiens
18 HBB 3043
Affinity Capture-MS Homo sapiens
19 KIF7 374654
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SNRPD2 6633
Cross-Linking-MS (XL-MS) Homo sapiens
21 RPS18 6222
Cross-Linking-MS (XL-MS) Homo sapiens
22 CAPZB 832
Affinity Capture-MS Homo sapiens
23 DDX23 9416
Cross-Linking-MS (XL-MS) Homo sapiens
24 PSMC5 5705
Proximity Label-MS Homo sapiens
25 INA 9118
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 DARS 1615
Cross-Linking-MS (XL-MS) Homo sapiens
27 PTPN12 5782
Affinity Capture-MS Homo sapiens
28 MCM2 4171
Affinity Capture-MS Homo sapiens
29 ATXN2L 11273
Cross-Linking-MS (XL-MS) Homo sapiens
30 NAA11 84779
Cross-Linking-MS (XL-MS) Homo sapiens
31 ABCC12  
Cross-Linking-MS (XL-MS) Homo sapiens
32 CALD1 800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ERC1 23085
Proximity Label-MS Homo sapiens
34 TNIK 23043
Two-hybrid Homo sapiens
35 CALM3 808
Affinity Capture-MS Homo sapiens
36 ABCD3 5825
Proximity Label-MS Homo sapiens
37 NUP93 9688
Proximity Label-MS Homo sapiens
38 EIF5B 9669
Cross-Linking-MS (XL-MS) Homo sapiens
39 KRT31 3881
Affinity Capture-MS Homo sapiens
40 LURAP1L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MLLT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 WDR45  
Proximity Label-MS Homo sapiens
43 CALR 811
Cross-Linking-MS (XL-MS) Homo sapiens
44 MECP2 4204
Affinity Capture-MS Homo sapiens
45 SCHIP1  
Affinity Capture-MS Homo sapiens
46 MYCN  
Affinity Capture-MS Homo sapiens
47 VWA8 23078
Affinity Capture-MS Homo sapiens
48 FBXW5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 KIF14 9928
Affinity Capture-MS Homo sapiens
50 STOML2 30968
Affinity Capture-MS Homo sapiens
51 RB1 5925
Reconstituted Complex Homo sapiens
52 KRT8 3856
Cross-Linking-MS (XL-MS) Homo sapiens
Proximity Label-MS Homo sapiens
53 AP3M1 26985
Proximity Label-MS Homo sapiens
54 KRT19 3880
Proximity Label-MS Homo sapiens
55 SLC25A10 1468
Proximity Label-MS Homo sapiens
56 PLOD3 8985
Cross-Linking-MS (XL-MS) Homo sapiens
57 KRT39  
Affinity Capture-MS Homo sapiens
58 PIBF1  
Cross-Linking-MS (XL-MS) Homo sapiens
59 PARK2  
Affinity Capture-MS Homo sapiens
60 MAP1B 4131
Cross-Linking-MS (XL-MS) Homo sapiens
61 MAPRE1 22919
Affinity Capture-MS Homo sapiens
62 GOLGA2 2801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SRRT 51593
Cross-Linking-MS (XL-MS) Homo sapiens
64 MYH9 4627
Cross-Linking-MS (XL-MS) Homo sapiens
65 IGHG1 3500
Affinity Capture-MS Homo sapiens
66 TTN 7273
Cross-Linking-MS (XL-MS) Homo sapiens
67 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SYNE1 23345
Cross-Linking-MS (XL-MS) Homo sapiens
69 PLK1 5347
Proximity Label-MS Homo sapiens
70 KRT14 3861
Affinity Capture-MS Homo sapiens
71 XPOT 11260
Proximity Label-MS Homo sapiens
72 SLC7A6 9057
Affinity Capture-MS Homo sapiens
73 CCDC39  
Cross-Linking-MS (XL-MS) Homo sapiens
74 DHX9 1660
Cross-Linking-MS (XL-MS) Homo sapiens
75 SLC25A4 291
Proximity Label-MS Homo sapiens
76 CAPN1 823
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MICU1 10367
Cross-Linking-MS (XL-MS) Homo sapiens
78 EPPK1 83481
Affinity Capture-MS Homo sapiens
79 PSG9  
Affinity Capture-MS Homo sapiens
80 TRIP13 9319
Proximity Label-MS Homo sapiens
81 DISC1 27185
Two-hybrid Homo sapiens
82 GAN 8139
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 HSPA5 3309
Cross-Linking-MS (XL-MS) Homo sapiens
84 S100P 6286
Affinity Capture-MS Homo sapiens
85 MAP7 9053
Affinity Capture-MS Homo sapiens
86 HADHA 3030
Cross-Linking-MS (XL-MS) Homo sapiens
87 FANCD2  
Affinity Capture-MS Homo sapiens
88 APOE 348
Reconstituted Complex Homo sapiens
89 KRT86  
Affinity Capture-MS Homo sapiens
90 SIRT7  
Affinity Capture-MS Homo sapiens
91 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 KRT32  
Affinity Capture-MS Homo sapiens
93 IQCB1  
Affinity Capture-MS Homo sapiens
94 PRMT8  
Affinity Capture-MS Homo sapiens
95 ZWINT  
Proximity Label-MS Homo sapiens
96 DES 1674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 CTTN 2017
Affinity Capture-MS Homo sapiens
98 SLC7A5 8140
Affinity Capture-MS Homo sapiens
99 RPL7 6129
Cross-Linking-MS (XL-MS) Homo sapiens
100 SS18  
Affinity Capture-MS Homo sapiens
101 KRT27 342574
Affinity Capture-MS Homo sapiens
102 DNAH9  
Cross-Linking-MS (XL-MS) Homo sapiens
103 CDC42BPA 8476
Affinity Capture-MS Homo sapiens
104 FOS 2353
Reconstituted Complex Homo sapiens
105 NEFL 4747
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
106 MYC  
Affinity Capture-MS Homo sapiens
107 SMAD2 4087
Reconstituted Complex Homo sapiens
108 MYO9B 4650
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
109 SART3 9733
Affinity Capture-MS Homo sapiens
110 UPF3B 65109
Cross-Linking-MS (XL-MS) Homo sapiens
111 MYH10 4628
Cross-Linking-MS (XL-MS) Homo sapiens
112 KRT222 125113
Affinity Capture-MS Homo sapiens
113 KRT16 3868
Affinity Capture-MS Homo sapiens
114 DRD2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
115 DSP 1832
Proximity Label-MS Homo sapiens
116 U2SURP 23350
Cross-Linking-MS (XL-MS) Homo sapiens
117 NES 10763
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 KRT18 3875
Cross-Linking-MS (XL-MS) Homo sapiens
Proximity Label-MS Homo sapiens
119 SLC25A11 8402
Proximity Label-MS Homo sapiens
120 LRRC49  
Proximity Label-MS Homo sapiens
121 NUP205 23165
Proximity Label-MS Homo sapiens
122 PLEC 5339
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 MCM7 4176
Proximity Label-MS Homo sapiens
124 SSH1  
Cross-Linking-MS (XL-MS) Homo sapiens
125 CCHCR1  
Affinity Capture-MS Homo sapiens
126 EVI5  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 DNAJA2 10294
Affinity Capture-MS Homo sapiens
128 RSPRY1 89970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 IKBKG 8517
Affinity Capture-MS Homo sapiens
130 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
131 SMURF1 57154
Affinity Capture-MS Homo sapiens
132 SQSTM1 8878
Affinity Capture-MS Homo sapiens
133 CCDC106  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 STK26 51765
Cross-Linking-MS (XL-MS) Homo sapiens
135 FAM83H 286077
Proximity Label-MS Homo sapiens
136 NEFH 4744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 IDE 3416
Affinity Capture-MS Homo sapiens
138 FHAD1 114827
Affinity Capture-MS Homo sapiens
139 PPP1R12A 4659
Cross-Linking-MS (XL-MS) Homo sapiens
140 KRT38 8687
Affinity Capture-MS Homo sapiens
141 KDELR2 11014
Proximity Label-MS Homo sapiens
142 CAD 790
Proximity Label-MS Homo sapiens
143 RAB11A 8766
Affinity Capture-MS Homo sapiens
144 RPRD1B 58490
Affinity Capture-MS Homo sapiens
145 BICD2 23299
Proximity Label-MS Homo sapiens
146 CCDC96  
Affinity Capture-MS Homo sapiens
147 NEFM 4741
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
148 VIM 7431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
149 PRMT1 3276
Affinity Capture-MS Homo sapiens
150 KRT7 3855
Affinity Capture-MS Homo sapiens
151 AIM1 202
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
152 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which NEFM is involved
No pathways found





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