Experiment description of studies that identified NEFM in sEVs

1 Experiment ID 267 MISEV standards ✘ Biophysical techniques ✔ TSG101|Alix Enriched markers ✔ CANX|HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25265333     Organism Homo sapiens Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J" Journal name J Proteome Res Publication year 2014 Sample Liver cancer cells Sample name Huh-7 - Transfected with HBx plasmids Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for NEFM

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PDHA1 5160 Affinity Capture-MS Homo sapiens 2 SLC25A13 10165 Proximity Label-MS Homo sapiens 3 KRT37 8688 Affinity Capture-MS Homo sapiens 4 DLG5 9231 Proximity Label-MS Homo sapiens 5 SENP3 26168 Proximity Label-MS Homo sapiens 6 TPGS1   Proximity Label-MS Homo sapiens 7 SETBP1   Cross-Linking-MS (XL-MS) Homo sapiens 8 FAM167A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 SYCE1   Affinity Capture-MS Homo sapiens 10 NUP188 23511 Proximity Label-MS Homo sapiens 11 DNTTIP1 116092 Affinity Capture-MS Homo sapiens 12 NUP62 23636 Affinity Capture-MS Homo sapiens 13 FASTKD5   Proximity Label-MS Homo sapiens 14 GFAP 2670 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 KIF20A 10112 Affinity Capture-MS Homo sapiens 16 WARS 7453 Cross-Linking-MS (XL-MS) Homo sapiens 17 SSB 6741 Cross-Linking-MS (XL-MS) Homo sapiens 18 HBB 3043 Affinity Capture-MS Homo sapiens 19 KIF7 374654 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 SNRPD2 6633 Cross-Linking-MS (XL-MS) Homo sapiens 21 RPS18 6222 Cross-Linking-MS (XL-MS) Homo sapiens 22 CAPZB 832 Affinity Capture-MS Homo sapiens 23 DDX23 9416 Cross-Linking-MS (XL-MS) Homo sapiens 24 PSMC5 5705 Proximity Label-MS Homo sapiens 25 INA 9118 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 DARS 1615 Cross-Linking-MS (XL-MS) Homo sapiens 27 PTPN12 5782 Affinity Capture-MS Homo sapiens 28 MCM2 4171 Affinity Capture-MS Homo sapiens 29 ATXN2L 11273 Cross-Linking-MS (XL-MS) Homo sapiens 30 NAA11 84779 Cross-Linking-MS (XL-MS) Homo sapiens 31 ABCC12   Cross-Linking-MS (XL-MS) Homo sapiens 32 CALD1 800 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 ERC1 23085 Proximity Label-MS Homo sapiens 34 TNIK 23043 Two-hybrid Homo sapiens 35 CALM3 808 Affinity Capture-MS Homo sapiens 36 ABCD3 5825 Proximity Label-MS Homo sapiens 37 NUP93 9688 Proximity Label-MS Homo sapiens 38 EIF5B 9669 Cross-Linking-MS (XL-MS) Homo sapiens 39 KRT31 3881 Affinity Capture-MS Homo sapiens 40 LURAP1L   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 MLLT6   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 WDR45   Proximity Label-MS Homo sapiens 43 CALR 811 Cross-Linking-MS (XL-MS) Homo sapiens 44 MECP2 4204 Affinity Capture-MS Homo sapiens 45 SCHIP1   Affinity Capture-MS Homo sapiens 46 MYCN   Affinity Capture-MS Homo sapiens 47 VWA8 23078 Affinity Capture-MS Homo sapiens 48 FBXW5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 KIF14 9928 Affinity Capture-MS Homo sapiens 50 STOML2 30968 Affinity Capture-MS Homo sapiens 51 RB1 5925 Reconstituted Complex Homo sapiens 52 KRT8 3856 Cross-Linking-MS (XL-MS) Homo sapiens Proximity Label-MS Homo sapiens 53 AP3M1 26985 Proximity Label-MS Homo sapiens 54 KRT19 3880 Proximity Label-MS Homo sapiens 55 SLC25A10 1468 Proximity Label-MS Homo sapiens 56 PLOD3 8985 Cross-Linking-MS (XL-MS) Homo sapiens 57 KRT39   Affinity Capture-MS Homo sapiens 58 PIBF1   Cross-Linking-MS (XL-MS) Homo sapiens 59 PARK2   Affinity Capture-MS Homo sapiens 60 MAP1B 4131 Cross-Linking-MS (XL-MS) Homo sapiens 61 MAPRE1 22919 Affinity Capture-MS Homo sapiens 62 GOLGA2 2801 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 SRRT 51593 Cross-Linking-MS (XL-MS) Homo sapiens 64 MYH9 4627 Cross-Linking-MS (XL-MS) Homo sapiens 65 IGHG1 3500 Affinity Capture-MS Homo sapiens 66 TTN 7273 Cross-Linking-MS (XL-MS) Homo sapiens 67 SYNC   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 SYNE1 23345 Cross-Linking-MS (XL-MS) Homo sapiens 69 PLK1 5347 Proximity Label-MS Homo sapiens 70 KRT14 3861 Affinity Capture-MS Homo sapiens 71 XPOT 11260 Proximity Label-MS Homo sapiens 72 SLC7A6 9057 Affinity Capture-MS Homo sapiens 73 CCDC39   Cross-Linking-MS (XL-MS) Homo sapiens 74 DHX9 1660 Cross-Linking-MS (XL-MS) Homo sapiens 75 SLC25A4 291 Proximity Label-MS Homo sapiens 76 CAPN1 823 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 77 MICU1 10367 Cross-Linking-MS (XL-MS) Homo sapiens 78 EPPK1 83481 Affinity Capture-MS Homo sapiens 79 PSG9   Affinity Capture-MS Homo sapiens 80 TRIP13 9319 Proximity Label-MS Homo sapiens 81 DISC1 27185 Two-hybrid Homo sapiens 82 GAN 8139 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 83 HSPA5 3309 Cross-Linking-MS (XL-MS) Homo sapiens 84 S100P 6286 Affinity Capture-MS Homo sapiens 85 MAP7 9053 Affinity Capture-MS Homo sapiens 86 HADHA 3030 Cross-Linking-MS (XL-MS) Homo sapiens 87 FANCD2   Affinity Capture-MS Homo sapiens 88 APOE 348 Reconstituted Complex Homo sapiens 89 KRT86   Affinity Capture-MS Homo sapiens 90 SIRT7   Affinity Capture-MS Homo sapiens 91 CCDC120   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 92 KRT32   Affinity Capture-MS Homo sapiens 93 IQCB1   Affinity Capture-MS Homo sapiens 94 PRMT8   Affinity Capture-MS Homo sapiens 95 ZWINT   Proximity Label-MS Homo sapiens 96 DES 1674 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 97 CTTN 2017 Affinity Capture-MS Homo sapiens 98 SLC7A5 8140 Affinity Capture-MS Homo sapiens 99 RPL7 6129 Cross-Linking-MS (XL-MS) Homo sapiens 100 SS18   Affinity Capture-MS Homo sapiens 101 KRT27 342574 Affinity Capture-MS Homo sapiens 102 DNAH9   Cross-Linking-MS (XL-MS) Homo sapiens 103 CDC42BPA 8476 Affinity Capture-MS Homo sapiens 104 FOS 2353 Reconstituted Complex Homo sapiens 105 NEFL 4747 Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 106 MYC   Affinity Capture-MS Homo sapiens 107 SMAD2 4087 Reconstituted Complex Homo sapiens 108 MYO9B 4650 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 109 SART3 9733 Affinity Capture-MS Homo sapiens 110 UPF3B 65109 Cross-Linking-MS (XL-MS) Homo sapiens 111 MYH10 4628 Cross-Linking-MS (XL-MS) Homo sapiens 112 KRT222 125113 Affinity Capture-MS Homo sapiens 113 KRT16 3868 Affinity Capture-MS Homo sapiens 114 DRD2   Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 115 DSP 1832 Proximity Label-MS Homo sapiens 116 U2SURP 23350 Cross-Linking-MS (XL-MS) Homo sapiens 117 NES 10763 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 118 KRT18 3875 Cross-Linking-MS (XL-MS) Homo sapiens Proximity Label-MS Homo sapiens 119 SLC25A11 8402 Proximity Label-MS Homo sapiens 120 LRRC49   Proximity Label-MS Homo sapiens 121 NUP205 23165 Proximity Label-MS Homo sapiens 122 PLEC 5339 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 123 MCM7 4176 Proximity Label-MS Homo sapiens 124 SSH1   Cross-Linking-MS (XL-MS) Homo sapiens 125 CCHCR1   Affinity Capture-MS Homo sapiens 126 EVI5   Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 127 DNAJA2 10294 Affinity Capture-MS Homo sapiens 128 RSPRY1 89970 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 129 IKBKG 8517 Affinity Capture-MS Homo sapiens 130 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 131 SMURF1 57154 Affinity Capture-MS Homo sapiens 132 SQSTM1 8878 Affinity Capture-MS Homo sapiens 133 CCDC106   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 134 STK26 51765 Cross-Linking-MS (XL-MS) Homo sapiens 135 FAM83H 286077 Proximity Label-MS Homo sapiens 136 NEFH 4744 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 137 IDE 3416 Affinity Capture-MS Homo sapiens 138 FHAD1 114827 Affinity Capture-MS Homo sapiens 139 PPP1R12A 4659 Cross-Linking-MS (XL-MS) Homo sapiens 140 KRT38 8687 Affinity Capture-MS Homo sapiens 141 KDELR2 11014 Proximity Label-MS Homo sapiens 142 CAD 790 Proximity Label-MS Homo sapiens 143 RAB11A 8766 Affinity Capture-MS Homo sapiens 144 RPRD1B 58490 Affinity Capture-MS Homo sapiens 145 BICD2 23299 Proximity Label-MS Homo sapiens 146 CCDC96   Affinity Capture-MS Homo sapiens 147 NEFM 4741 Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 148 VIM 7431 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 149 PRMT1 3276 Affinity Capture-MS Homo sapiens 150 KRT7 3855 Affinity Capture-MS Homo sapiens 151 AIM1 202 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 152 HNRNPA2B1 3181 Cross-Linking-MS (XL-MS) Homo sapiens View the network

Pathways in which NEFM is involved

No pathways found