Gene description for ACOX1
Gene name acyl-CoA oxidase 1, palmitoyl
Gene symbol ACOX1
Other names/aliases ACOX
PALMCOX
SCOX
Species Homo sapiens
 Database cross references - ACOX1
ExoCarta ExoCarta_51
Vesiclepedia VP_51
Entrez Gene 51
HGNC 119
MIM 609751
UniProt Q15067  
 ACOX1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ACOX1
Molecular Function
    acyl-CoA oxidase activity GO:0003997 IDA
    acyl-CoA oxidase activity GO:0003997 IGI
    acyl-CoA oxidase activity GO:0003997 IMP
    fatty acid binding GO:0005504 IBA
    protein binding GO:0005515 IPI
    palmitoyl-CoA oxidase activity GO:0016401 IBA
    palmitoyl-CoA oxidase activity GO:0016401 IDA
    PDZ domain binding GO:0030165 IDA
    protein homodimerization activity GO:0042803 IDA
    flavin adenine dinucleotide binding GO:0050660 IBA
    FAD binding GO:0071949 IDA
    FAD binding GO:0071949 IEA
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IBA
    very long-chain fatty acid metabolic process GO:0000038 IMP
    generation of precursor metabolites and energy GO:0006091 IMP
    lipid metabolic process GO:0006629 IDA
    prostaglandin metabolic process GO:0006693 IMP
    spermatogenesis GO:0007283 IEA
    fatty acid catabolic process GO:0009062 IMP
    peroxisome fission GO:0016559 IGI
    fatty acid oxidation GO:0019395 IGI
    fatty acid oxidation GO:0019395 IMP
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 IBA
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 IMP
    cholesterol homeostasis GO:0042632 IGI
    hydrogen peroxide biosynthetic process GO:0050665 IMP
    lipid homeostasis GO:0055088 IDA
    lipid homeostasis GO:0055088 IGI
    very long-chain fatty acid beta-oxidation GO:0140493 IMP
Subcellular Localization
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 IEA
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified ACOX1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ACOX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LHFPL4  
Affinity Capture-MS Homo sapiens
2 IDH1 3417
Co-fractionation Homo sapiens
3 ARPC2 10109
Co-fractionation Homo sapiens
4 ACAA1 30
Affinity Capture-MS Homo sapiens
5 LAP3 51056
Co-fractionation Homo sapiens
6 NUP62 23636
Co-fractionation Homo sapiens
7 EPB41L5 57669
Affinity Capture-MS Homo sapiens
8 ALDH2 217
Co-fractionation Homo sapiens
9 PACSIN2 11252
Co-fractionation Homo sapiens
10 GSTA1 2938
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAD1 3898
Affinity Capture-MS Homo sapiens
12 ECI1 1632
Co-fractionation Homo sapiens
13 PHYH 5264
Affinity Capture-MS Homo sapiens
14 GRHPR 9380
Co-fractionation Homo sapiens
15 CHCHD4  
Co-fractionation Homo sapiens
16 PTDSS1 9791
Affinity Capture-MS Homo sapiens
17 HSPA2 3306
Co-fractionation Homo sapiens
18 TAOK3 51347
Co-fractionation Homo sapiens
19 MCM2 4171
Affinity Capture-MS Homo sapiens
20 RAF1 5894
Affinity Capture-MS Homo sapiens
21 DUSP14 11072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 RECK 8434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MRPL12 6182
Co-fractionation Homo sapiens
24 FAM136A 84908
Affinity Capture-MS Homo sapiens
25 PGD 5226
Co-fractionation Homo sapiens
26 PALLD 23022
Co-fractionation Homo sapiens
27 RAB6B 51560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 C18orf21  
Affinity Capture-MS Homo sapiens
29 DDX19B 11269
Affinity Capture-MS Homo sapiens
30 PARK2  
Affinity Capture-MS Homo sapiens
31 HRSP12 10247
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RAB30 27314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SLC25A20 788
Co-fractionation Homo sapiens
34 PNMA1 9240
Affinity Capture-MS Homo sapiens
35 OGT 8473
Reconstituted Complex Homo sapiens
36 SSUH2  
Affinity Capture-MS Homo sapiens
37 APBA1  
Affinity Capture-MS Homo sapiens
38 RPLP0 6175
Affinity Capture-MS Homo sapiens
39 MBNL2 10150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 AGPS 8540
Proximity Label-MS Homo sapiens
41 FOXS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C7orf49  
Affinity Capture-MS Homo sapiens
43 ANLN 54443
Affinity Capture-MS Homo sapiens
44 CAT 847
Co-fractionation Homo sapiens
45 TMED9 54732
Co-fractionation Homo sapiens
46 SLC2A4  
Affinity Capture-MS Homo sapiens
47 FAM60A  
Affinity Capture-MS Homo sapiens
48 GPX1 2876
Co-fractionation Homo sapiens
49 ASS1 445
Co-fractionation Homo sapiens
50 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
51 TYSND1  
Biochemical Activity Homo sapiens
52 COX6B1 1340
Co-fractionation Homo sapiens
53 ECHS1 1892
Co-fractionation Homo sapiens
54 ADAMTSL1 92949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CRAT 1384
Co-fractionation Homo sapiens
56 UBE2D2 7322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LACRT 90070
Affinity Capture-MS Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 PPWD1 23398
Co-fractionation Homo sapiens
60 RAB11B 9230
Affinity Capture-MS Homo sapiens
61 AADAT  
Co-fractionation Homo sapiens
62 ENO2 2026
Co-fractionation Homo sapiens
63 STX17 55014
Affinity Capture-MS Homo sapiens
64 CDKN2C  
Affinity Capture-MS Homo sapiens
65 GSR 2936
Co-fractionation Homo sapiens
66 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
67 CS 1431
Co-fractionation Homo sapiens
68 ARL2 402
Affinity Capture-MS Homo sapiens
69 TXNDC16 57544
Affinity Capture-MS Homo sapiens
70 UBA5 79876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TXNRD2 10587
Co-fractionation Homo sapiens
72 M6PR 4074
Co-fractionation Homo sapiens
73 ASL 435
Co-fractionation Homo sapiens
74 LITAF 9516
Affinity Capture-MS Homo sapiens
75 BOC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SFXN1 94081
Co-fractionation Homo sapiens
77 MCFD2 90411
Co-fractionation Homo sapiens
78 KHDRBS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 MBNL1 4154
Affinity Capture-MS Homo sapiens
80 HNRNPF 3185
Affinity Capture-MS Homo sapiens
81 ACBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 PEX5 5830
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
83 OR2A4 79541
Affinity Capture-MS Homo sapiens
84 HS1BP3 64342
Affinity Capture-MS Homo sapiens
85 ATOH1  
Affinity Capture-MS Homo sapiens
86 CCR1  
Affinity Capture-MS Homo sapiens
87 PCYT1A 5130
Affinity Capture-MS Homo sapiens
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