Gene description for ITSN1
Gene name intersectin 1 (SH3 domain protein)
Gene symbol ITSN1
Other names/aliases ITSN
SH3D1A
SH3P17
Species Homo sapiens
 Database cross references - ITSN1
ExoCarta ExoCarta_6453
Entrez Gene 6453
HGNC 6183
MIM 602442
UniProt Q15811  
 ITSN1 identified in exosomes derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for ITSN1
Molecular Function
    calcium ion binding GO:0005509 NAS
    proline-rich region binding GO:0070064 IPI
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 NAS
    kinase activator activity GO:0019209 IEA
    protein binding GO:0005515 IPI
    protein complex scaffold GO:0032947 IDA
Biological Process
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of protein kinase B signaling GO:0051897 IEA
    axon guidance GO:0007411 TAS
    synaptic vesicle endocytosis GO:0048488 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    apoptotic signaling pathway GO:0097190 TAS
    negative regulation of neuron apoptotic process GO:0043524 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of GTPase activity GO:0043547 NAS
    positive regulation of phosphorylation GO:0042327 IEA
Subcellular Localization
    synapse GO:0045202 IEA
    cytosol GO:0005829 TAS
    coated pit GO:0005905 IDA
    cell junction GO:0030054 IEA
    endocytic vesicle GO:0030139 IEA
    lamellipodium GO:0030027 IEA
    neuron projection GO:0043005 IEA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified ITSN1 in exosomes
1
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITSN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SCAMP1 9522
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 CDC42 998
Invitro Homo sapiens
3 SNAP23 8773
Reconstituted Complex Homo sapiens
4 EPHB2 2048
Affinity Capture-Western Homo sapiens
5 DISC1 27185
Two-hybrid Homo sapiens
6 MRPL20  
Two-hybrid Homo sapiens
7 STON2 85439
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 TSG101 7251
Two-hybrid Homo sapiens
9 KIF5A 3798
Two-hybrid Homo sapiens
10 EPS15 2060
Invivo Homo sapiens
Two-hybrid Homo sapiens
11 SOS1  
Invivo Homo sapiens
Invitro Homo sapiens
12 SNAP25 6616
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 EEF1A1 1915
Two-hybrid Homo sapiens
14 UNC119  
Two-hybrid Homo sapiens
15 SNX5 27131
Two-hybrid Homo sapiens
16 SMARCC2 6601
Two-hybrid Homo sapiens
17 WASL 8976
Invivo Homo sapiens
Invitro Homo sapiens
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