Experiment description of studies that identified DDX3Y in sEVs

1 Experiment ID 1275 MISEV standards Biophysical techniques ✔ CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 1280 MISEV standards Biophysical techniques ✔ TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

5 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

6 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for DDX3Y

Protein Interactor ExoCarta ID Identification method PubMed Species 1 POT1   Affinity Capture-MS Homo sapiens 2 VPS35 55737 Affinity Capture-MS Homo sapiens 3 MECP2 4204 Affinity Capture-MS Homo sapiens 4 EIF3F 8665 Affinity Capture-MS Homo sapiens 5 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 6 ZSCAN23   Affinity Capture-MS Homo sapiens 7 SHBG 6462 Two-hybrid Homo sapiens 8 METTL14   Affinity Capture-MS Homo sapiens 9 CCNB2   Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 10 WWOX 51741 Affinity Capture-MS Homo sapiens 11 CHMP4C 92421 Affinity Capture-MS Homo sapiens 12 TSPO 706 Affinity Capture-MS Homo sapiens 13 RPAP1   Affinity Capture-MS Homo sapiens 14 DAB2IP 153090 Affinity Capture-MS Homo sapiens 15 AIM1L   Affinity Capture-MS Homo sapiens 16 CLDN3 1365 Affinity Capture-MS Homo sapiens 17 LGALS9B 284194 Affinity Capture-MS Homo sapiens 18 C3orf14   Affinity Capture-MS Homo sapiens 19 VHL   Affinity Capture-MS Homo sapiens 20 FBXO6 26270 Affinity Capture-MS Homo sapiens 21 PICALM 8301 Affinity Capture-MS Homo sapiens 22 STK38L 23012 Affinity Capture-MS Homo sapiens 23 RNF10   Affinity Capture-MS Homo sapiens 24 ZC3H11A   Affinity Capture-MS Homo sapiens 25 ESR1   Affinity Capture-MS Homo sapiens 26 USP11 8237 Affinity Capture-MS Homo sapiens 27 NUDCD2 134492 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 RNH1 6050 Affinity Capture-MS Homo sapiens 29 FAM107A   Affinity Capture-MS Homo sapiens 30 BRCA2 675 Affinity Capture-MS Homo sapiens 31 PCBP1 5093 Affinity Capture-MS Homo sapiens 32 CIRH1A 84916 Affinity Capture-MS Homo sapiens 33 SERBP1 26135 Affinity Capture-MS Homo sapiens 34 EIF4B 1975 Affinity Capture-MS Homo sapiens 35 ATG4B 23192 Affinity Capture-MS Homo sapiens 36 MCM2 4171 Affinity Capture-MS Homo sapiens 37 SMAD3 4088 Affinity Capture-MS Homo sapiens 38 AFAP1L1 134265 Affinity Capture-MS Homo sapiens 39 PTPDC1   Affinity Capture-MS Homo sapiens 40 ESRRG   Affinity Capture-MS Homo sapiens 41 METTL3   Affinity Capture-MS Homo sapiens 42 SMAD2 4087 Affinity Capture-MS Homo sapiens 43 SAP25   Affinity Capture-MS Homo sapiens 44 CRY2   Affinity Capture-MS Homo sapiens 45 C9orf72   Affinity Capture-MS Homo sapiens 46 BIRC7   Affinity Capture-MS Homo sapiens 47 RNF34   Affinity Capture-MS Homo sapiens 48 ERP27   Affinity Capture-MS Homo sapiens 49 TIMP3 7078 Affinity Capture-MS Homo sapiens 50 POLR2A 5430 Affinity Capture-MS Homo sapiens View the network

Pathways in which DDX3Y is involved

No pathways found