Gene description for SMC1A
Gene name structural maintenance of chromosomes 1A
Gene symbol SMC1A
Other names/aliases CDLS2
DXS423E
SB1.8
SMC1
SMC1L1
SMC1alpha
SMCB
Species Homo sapiens
 Database cross references - SMC1A
ExoCarta ExoCarta_8243
Entrez Gene 8243
HGNC 11111
MIM 300040
UniProt Q14683  
 SMC1A identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SMC1A
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    chromatin binding GO:0003682 IDA
    protein binding GO:0005515 IPI
    microtubule motor activity GO:0003777 NAS
    ATP binding GO:0005524 IEA
    protein heterodimerization activity GO:0046982 IPI
    mediator complex binding GO:0036033 IEA
Biological Process
    sister chromatid cohesion GO:0007062 IMP
    DNA repair GO:0006281 TAS
    mitotic cell cycle checkpoint GO:0007093 IDA
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    gene expression GO:0010467 TAS
    cell division GO:0051301 IEA
    signal transduction in response to DNA damage GO:0042770 IDA
    protein sumoylation GO:0016925 TAS
    response to radiation GO:0009314 IEP
    RNA splicing GO:0008380 TAS
    meiotic nuclear division GO:0007126 ISS
    mitotic sister chromatid cohesion GO:0007064 TAS
    stem cell maintenance GO:0019827 IEA
    mitotic sister chromatid segregation GO:0000070 TAS
    mitotic cell cycle GO:0000278 TAS
    negative regulation of DNA endoreduplication GO:0032876 IMP
    mitotic spindle organization GO:0007052 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
Subcellular Localization
    chromosome GO:0005694 TAS
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    chromosome, centromeric region GO:0000775 TAS
    kinetochore GO:0000776 IDA
    meiotic cohesin complex GO:0030893 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    condensed chromosome kinetochore GO:0000777 IEA
    condensed nuclear chromosome GO:0000794 TAS
    cytoplasm GO:0005737 IDA
    cohesin core heterodimer GO:0008280 TAS
 Experiment description of studies that identified SMC1A in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMC1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDC80 10403
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 CASP4  
Affinity Capture-MS Homo sapiens
3 CHTF18 63922
Affinity Capture-Western Homo sapiens
4 SYCP3  
Affinity Capture-Western Homo sapiens
5 SMC3 9126
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 STYXL1  
Affinity Capture-MS Homo sapiens
7 REC8  
Affinity Capture-Western Homo sapiens
8 NUMA1 4926
Affinity Capture-Western Homo sapiens
9 ATM  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 CDK4 1019
Affinity Capture-MS Homo sapiens
11 MSH6 2956
Reconstituted Complex Homo sapiens
12 RAD21 5885
Affinity Capture-Western Homo sapiens
13 SMARCA5 8467
Co-purification Homo sapiens
14 STAG1  
Co-purification Homo sapiens
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