Gene description for PRKAA1
Gene name protein kinase, AMP-activated, alpha 1 catalytic subunit
Gene symbol PRKAA1
Other names/aliases AMPK
AMPKa1
Species Homo sapiens
 Database cross references - PRKAA1
ExoCarta ExoCarta_5562
Entrez Gene 5562
HGNC 9376
MIM 602739
UniProt Q13131  
 PRKAA1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PRKAA1
Molecular Function
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 ISS
    [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity GO:0047322 IEA
    kinase binding GO:0019900 IEA
    [acetyl-CoA carboxylase] kinase activity GO:0050405 IEA
    histone serine kinase activity GO:0035174 ISS
    protein kinase activity GO:0004672 IDA
    cAMP-dependent protein kinase activity GO:0004691 NAS
    tau-protein kinase activity GO:0050321 IEA
    AMP-activated protein kinase activity GO:0004679 IDA
    metal ion binding GO:0046872 IEA
    protein C-terminus binding GO:0008022 IEA
    ATP binding GO:0005524 IEA
Biological Process
    rhythmic process GO:0048511 IEA
    response to hypoxia GO:0001666 NAS
    insulin receptor signaling pathway GO:0008286 TAS
    cellular response to glucose starvation GO:0042149 ISS
    glucose metabolic process GO:0006006 IEA
    response to UV GO:0009411 IEA
    positive regulation of autophagy GO:0010508 ISS
    response to camptothecin GO:1901563 IEA
    cellular response to ethanol GO:0071361 IEA
    fatty acid oxidation GO:0019395 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of lipid catabolic process GO:0050995 ISS
    positive regulation of glycolytic process GO:0045821 ISS
    positive regulation of cholesterol biosynthetic process GO:0045542 NAS
    positive regulation of cell proliferation GO:0008284 IEA
    autophagy GO:0006914 IEA
    signal transduction GO:0007165 TAS
    cellular response to nutrient levels GO:0031669 ISS
    cellular response to hypoxia GO:0071456 IEA
    activation of MAPK activity GO:0000187 NAS
    regulation of energy homeostasis GO:2000505 ISS
    fatty acid biosynthetic process GO:0006633 IEA
    lipid biosynthetic process GO:0008610 ISS
    intracellular signal transduction GO:0035556 IBA
    cell cycle arrest GO:0007050 TAS
    protein heterooligomerization GO:0051291 IEA
    response to gamma radiation GO:0010332 ISS
    negative regulation of glucose import in response to insulin stimulus GO:2001274 IEA
    cholesterol biosynthetic process GO:0006695 IEA
    protein phosphorylation GO:0006468 IDA
    cold acclimation GO:0009631 IEA
    histone-serine phosphorylation GO:0035404 IBA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    negative regulation of glucosylceramide biosynthetic process GO:0046318 NAS
    gene expression GO:0010467 TAS
    response to activity GO:0014823 IEA
    regulation of vesicle-mediated transport GO:0060627 IEA
    negative regulation of TOR signaling GO:0032007 ISS
    glucose homeostasis GO:0042593 ISS
    negative regulation of apoptotic process GO:0043066 ISS
    response to caffeine GO:0031000 IEA
    fatty acid homeostasis GO:0055089 ISS
    Wnt signaling pathway GO:0016055 IEA
    regulation of circadian rhythm GO:0042752 ISS
Subcellular Localization
    apical plasma membrane GO:0016324 IEA
    intracellular GO:0005622 IC
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 ISS
    AMP-activated protein kinase complex GO:0031588 ISS
 Experiment description of studies that identified PRKAA1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKAA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TSC2 7249
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 MTOR 2475
Protein-peptide Homo sapiens
3 KIAA1961  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
4 CFTR  
Reconstituted Complex Homo sapiens
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