Gene description for PRKAA1
Gene name protein kinase, AMP-activated, alpha 1 catalytic subunit
Gene symbol PRKAA1
Other names/aliases AMPK
AMPKa1
Species Homo sapiens
 Database cross references - PRKAA1
ExoCarta ExoCarta_5562
Vesiclepedia VP_5562
Entrez Gene 5562
HGNC 9376
MIM 602739
UniProt Q13131  
 PRKAA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for PRKAA1
Molecular Function
    chromatin binding GO:0003682 ISS
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IDA
    AMP-activated protein kinase activity GO:0004679 ISS
    AMP-activated protein kinase activity GO:0004679 TAS
    cAMP-dependent protein kinase activity GO:0004691 NAS
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-containing complex binding GO:0044877 IEA
    metal ion binding GO:0046872 IEA
    [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity GO:0047322 IEA
    tau protein binding GO:0048156 NAS
    tau-protein kinase activity GO:0050321 NAS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 ISS
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    response to hypoxia GO:0001666 NAS
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 IDA
    glucose metabolic process GO:0006006 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    fatty acid biosynthetic process GO:0006633 IEA
    cholesterol biosynthetic process GO:0006695 IEA
    autophagy GO:0006914 IEA
    signal transduction GO:0007165 TAS
    positive regulation of cell population proliferation GO:0008284 IEA
    lipid biosynthetic process GO:0008610 ISS
    response to UV GO:0009411 IEA
    cold acclimation GO:0009631 IEA
    response to gamma radiation GO:0010332 ISS
    positive regulation of autophagy GO:0010508 IDA
    positive regulation of autophagy GO:0010508 IMP
    positive regulation of autophagy GO:0010508 ISS
    positive regulation of gene expression GO:0010628 IEA
    negative regulation of gene expression GO:0010629 ISS
    response to activity GO:0014823 IEA
    Wnt signaling pathway GO:0016055 IEA
    fatty acid oxidation GO:0019395 IEA
    response to caffeine GO:0031000 IEA
    cellular response to nutrient levels GO:0031669 IDA
    cellular response to nutrient levels GO:0031669 ISS
    negative regulation of TOR signaling GO:0032007 IBA
    negative regulation of TOR signaling GO:0032007 ISS
    cellular response to stress GO:0033554 IDA
    cellular response to oxidative stress GO:0034599 ISS
    cellular response to glucose starvation GO:0042149 IBA
    cellular response to glucose starvation GO:0042149 IDA
    glucose homeostasis GO:0042593 ISS
    regulation of circadian rhythm GO:0042752 ISS
    negative regulation of apoptotic process GO:0043066 ISS
    response to estrogen GO:0043627 IEA
    positive regulation of cholesterol biosynthetic process GO:0045542 NAS
    positive regulation of glycolytic process GO:0045821 ISS
    positive regulation of DNA-templated transcription GO:0045893 ISS
    negative regulation of glucosylceramide biosynthetic process GO:0046318 NAS
    negative regulation of insulin receptor signaling pathway GO:0046627 IEA
    rhythmic process GO:0048511 IEA
    positive regulation of skeletal muscle tissue development GO:0048643 IEA
    positive regulation of T cell activation GO:0050870 IDA
    negative regulation of lipid catabolic process GO:0050995 ISS
    fatty acid homeostasis GO:0055089 ISS
    regulation of vesicle-mediated transport GO:0060627 IEA
    motor behavior GO:0061744 IGI
    CAMKK-AMPK signaling cascade GO:0061762 IC
    CAMKK-AMPK signaling cascade GO:0061762 IMP
    CAMKK-AMPK signaling cascade GO:0061762 TAS
    regulation of stress granule assembly GO:0062028 IEA
    neuron cellular homeostasis GO:0070050 IGI
    cellular response to hydrogen peroxide GO:0070301 IEA
    regulation of microtubule cytoskeleton organization GO:0070507 ISS
    cellular response to calcium ion GO:0071277 ISS
    cellular response to glucose stimulus GO:0071333 ISS
    cellular response to ethanol GO:0071361 IEA
    cellular response to prostaglandin E stimulus GO:0071380 IEA
    cellular response to hypoxia GO:0071456 IEA
    cellular response to xenobiotic stimulus GO:0071466 IEA
    energy homeostasis GO:0097009 ISS
    energy homeostasis GO:0097009 TAS
    positive regulation of mitochondrial transcription GO:1903109 IEA
    positive regulation of protein localization GO:1903829 ISS
    negative regulation of hepatocyte apoptotic process GO:1903944 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 IEA
    positive regulation of adipose tissue development GO:1904179 ISS
    negative regulation of TORC1 signaling GO:1904262 IDA
    negative regulation of tubulin deacetylation GO:1904428 ISS
    lipid droplet disassembly GO:1905691 IDA
    protein localization to lipid droplet GO:1990044 IDA
    positive regulation of peptidyl-lysine acetylation GO:2000758 ISS
Subcellular Localization
    chromatin GO:0000785 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    apical plasma membrane GO:0016324 IEA
    nuclear speck GO:0016607 IDA
    axon GO:0030424 ISS
    dendrite GO:0030425 ISS
    nucleotide-activated protein kinase complex GO:0031588 IBA
    nucleotide-activated protein kinase complex GO:0031588 IPI
    nucleotide-activated protein kinase complex GO:0031588 ISS
    nucleotide-activated protein kinase complex GO:0031588 TAS
    neuronal cell body GO:0043025 ISS
 Experiment description of studies that identified PRKAA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
21
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
24
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
34
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
36
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKAA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRBN  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 INSIG1  
Affinity Capture-Western Homo sapiens
3 ATP6V1B2 526
Co-fractionation Homo sapiens
4 GATA1  
Two-hybrid Homo sapiens
5 CRTC2 200186
Biochemical Activity Homo sapiens
6 RBPMS 11030
Two-hybrid Homo sapiens
7 SHC1 6464
Affinity Capture-Western Homo sapiens
8 ROPN1  
Two-hybrid Homo sapiens
9 TSC2 7249
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
10 RFX6  
Two-hybrid Homo sapiens
11 SRPK2 6733
Biochemical Activity Homo sapiens
12 AES 166
Two-hybrid Homo sapiens
13 FMR1 2332
Affinity Capture-MS Homo sapiens
14 YWHAE 7531
Affinity Capture-MS Homo sapiens
15 Prkag2 108099
Two-hybrid Mus musculus
16 MORC4  
Two-hybrid Homo sapiens
17 Hspb1  
Two-hybrid Mus musculus
18 RARRES3  
Affinity Capture-MS Homo sapiens
19 BRSK1  
Affinity Capture-MS Homo sapiens
20 C9orf78 51759
Affinity Capture-MS Homo sapiens
21 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
22 PSMD11 5717
Biochemical Activity Homo sapiens
23 INCENP 3619
Affinity Capture-MS Homo sapiens
24 PRKAG2 51422
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 NUAK2  
Affinity Capture-MS Homo sapiens
26 Arhgap22  
Two-hybrid Mus musculus
27 SUN2 25777
Affinity Capture-MS Homo sapiens
28 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
29 DDA1  
Affinity Capture-MS Homo sapiens
30 TRMU  
Affinity Capture-MS Homo sapiens
31 Aprt 11821
Two-hybrid Mus musculus
32 CTBP1 1487
Two-hybrid Homo sapiens
33 MCM2 4171
Affinity Capture-MS Homo sapiens
34 SOGA1 140710
Affinity Capture-Western Homo sapiens
35 STIM2 57620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 FBXW7  
Affinity Capture-MS Homo sapiens
37 KRT40  
Two-hybrid Homo sapiens
38 SCRIB 23513
Affinity Capture-MS Homo sapiens
39 Cpe 12876
Two-hybrid Mus musculus
40 Prkag1  
Two-hybrid Mus musculus
41 SLC31A1 1317
Affinity Capture-Western Homo sapiens
42 EIF5B 9669
Affinity Capture-MS Homo sapiens
43 MTUS2 23281
Two-hybrid Homo sapiens
44 Ncstn 59287
Affinity Capture-MS Mus musculus
45 SIK3 23387
Affinity Capture-MS Homo sapiens
46 PPM1A 5494
Biochemical Activity Homo sapiens
47 TULP3 7289
Affinity Capture-MS Homo sapiens
48 BRD4 23476
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 POLR2C 5432
Proximity Label-MS Homo sapiens
50 USP2  
Affinity Capture-MS Homo sapiens
51 ABI1 10006
Affinity Capture-Western Homo sapiens
52 MTIF2 4528
Affinity Capture-MS Homo sapiens
53 DVL2 1856
Two-hybrid Homo sapiens
54 LAMTOR1 55004
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 Grn  
Two-hybrid Mus musculus
56 SKOR1  
Affinity Capture-MS Homo sapiens
57 INO80E  
Two-hybrid Homo sapiens
58 USP10 9100
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
59 POLR3K  
Affinity Capture-MS Homo sapiens
60 PARK2  
Affinity Capture-MS Homo sapiens
61 SLC9A5  
Affinity Capture-MS Homo sapiens
62 TUBA1A 7846
Affinity Capture-MS Homo sapiens
63 PRKAG1 5571
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 GOLGA2 2801
Two-hybrid Homo sapiens
65 PNMA5 114824
Two-hybrid Homo sapiens
66 CERS2 29956
Affinity Capture-MS Homo sapiens
67 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
68 ACACA 31
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
69 RPL35 11224
Affinity Capture-MS Homo sapiens
70 RFESD  
Affinity Capture-MS Homo sapiens
71 Cherp  
Two-hybrid Mus musculus
72 VCP 7415
Affinity Capture-MS Homo sapiens
73 NTRK1 4914
Affinity Capture-MS Homo sapiens
74 Tfpt  
Two-hybrid Mus musculus
75 PPP2CA 5515
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
76 CAMKK1  
Biochemical Activity Homo sapiens
77 ULK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
78 MID1IP1  
Affinity Capture-MS Homo sapiens
79 AKR1A1 10327
Affinity Capture-MS Homo sapiens
80 SUB1 10923
Affinity Capture-MS Homo sapiens
81 Sde2  
Two-hybrid Mus musculus
82 CDX4  
Two-hybrid Homo sapiens
83 SND1 27044
Affinity Capture-MS Homo sapiens
84 HDAC5 10014
Biochemical Activity Homo sapiens
85 BRSK2  
Affinity Capture-MS Homo sapiens
86 ZBED1  
Two-hybrid Homo sapiens
87 SSX2IP  
Two-hybrid Homo sapiens
88 Thap7  
Two-hybrid Mus musculus
89 FNIP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
90 TOMM34 10953
Two-hybrid Homo sapiens
91 Cit 12704
Affinity Capture-MS Mus musculus
92 Nab2  
Two-hybrid Mus musculus
93 PPM1E  
Biochemical Activity Homo sapiens
94 UBE2H 7328
Affinity Capture-MS Homo sapiens
95 MAGEA11  
Affinity Capture-Western Homo sapiens
96 IKZF3  
Two-hybrid Homo sapiens
97 PPARG 5468
Affinity Capture-Western Homo sapiens
98 CLIP4  
Affinity Capture-MS Homo sapiens
99 Trp53  
Biochemical Activity Mus musculus
100 PRKAA2 5563
Affinity Capture-MS Homo sapiens
101 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
102 ASPM 259266
Affinity Capture-MS Homo sapiens
103 YWHAZ 7534
Affinity Capture-MS Homo sapiens
104 MARK4  
Affinity Capture-MS Homo sapiens
105 BHLHE40  
Two-hybrid Homo sapiens
106 EGFR 1956
Affinity Capture-MS Homo sapiens
107 TRIM27  
Two-hybrid Homo sapiens
108 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 HMBOX1  
Two-hybrid Homo sapiens
111 NUAK1  
Affinity Capture-MS Homo sapiens
112 MAP3K7  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
113 THAP1  
Two-hybrid Homo sapiens
114 Prkab1  
Two-hybrid Mus musculus
115 FKBP5 2289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 GADD45GIP1  
Affinity Capture-MS Homo sapiens
117 L3MBTL3  
Two-hybrid Homo sapiens
118 RPA3 6119
Proximity Label-MS Homo sapiens
119 FXR2 9513
Affinity Capture-MS Homo sapiens
120 RAD54B  
Two-hybrid Homo sapiens
121 ATP6V1A 523
Co-fractionation Homo sapiens
122 MTOR 2475
Protein-peptide Homo sapiens
123 Wdr62  
Two-hybrid Mus musculus
124 HSPA4 3308
Affinity Capture-MS Homo sapiens
125 Nedd1  
Two-hybrid Mus musculus
126 PRKAB2 5565
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 SIK2  
Affinity Capture-MS Homo sapiens
128 MDM4  
Biochemical Activity Homo sapiens
129 C7orf60 154743
Affinity Capture-MS Homo sapiens
130 DPYSL3 1809
Affinity Capture-MS Homo sapiens
131 Sorbs1  
Two-hybrid Mus musculus
132 SUPT16H 11198
Affinity Capture-MS Homo sapiens
133 CCDC8  
Affinity Capture-MS Homo sapiens
134 Emilin1 100952
Two-hybrid Mus musculus
135 PPM1F 9647
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
136 PA2G4 5036
Affinity Capture-MS Homo sapiens
137 MARK3 4140
Affinity Capture-MS Homo sapiens
138 TRIP6 7205
Two-hybrid Homo sapiens
139 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 PLEKHA4 57664
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
141 PARP1 142
Affinity Capture-MS Homo sapiens
142 CCKBR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 CFTR 1080
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
144 CUTA 51596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 TP53 7157
Affinity Capture-Western Homo sapiens
146 HOMEZ  
Two-hybrid Homo sapiens
147 VPS52 6293
Two-hybrid Homo sapiens
148 MCL1 4170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 Mtfr2  
Two-hybrid Mus musculus
150 Kif1c  
Two-hybrid Mus musculus
151 CDC37 11140
Affinity Capture-MS Homo sapiens
152 Abi1 11308
Two-hybrid Mus musculus
153 EDEM1  
Affinity Capture-MS Homo sapiens
154 RPTOR 57521
Biochemical Activity Homo sapiens
155 G3BP1 10146
Affinity Capture-MS Homo sapiens
156 INSR 3643
Affinity Capture-Western Homo sapiens
157 PRKAB1 5564
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
159 UBXN11  
Two-hybrid Homo sapiens
160 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 TSC22D4 81628
Two-hybrid Homo sapiens
162 SNRK  
Affinity Capture-MS Homo sapiens
163 Bhlhe40  
Two-hybrid Mus musculus
164 Rc3h1  
Two-hybrid Mus musculus
165 NUP50 10762
Co-fractionation Homo sapiens
166 Lzts2  
Two-hybrid Mus musculus
167 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
168 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
169 MARK2 2011
Affinity Capture-MS Homo sapiens
170 MARK1 4139
Affinity Capture-MS Homo sapiens
171 RIMBP3 85376
Two-hybrid Homo sapiens
172 Xrn2  
Two-hybrid Mus musculus
173 ACACB 32
Affinity Capture-MS Homo sapiens
174 STK11 6794
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
175 TXNIP 10628
Biochemical Activity Homo sapiens
176 PRKAG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
178 PHC2  
Two-hybrid Homo sapiens
179 MAST3  
Affinity Capture-MS Homo sapiens
180 VPS37B 79720
Two-hybrid Homo sapiens
181 AP5Z1 9907
Affinity Capture-MS Homo sapiens
182 PPARGC1A  
Affinity Capture-Western Homo sapiens
183 EPM2A  
Biochemical Activity Homo sapiens
184 Trip6  
Two-hybrid Mus musculus
185 NOTCH1 4851
Affinity Capture-MS Homo sapiens
186 SIK1  
Affinity Capture-MS Homo sapiens
187 Maged1  
Two-hybrid Mus musculus
188 EP300 2033
Affinity Capture-Western Homo sapiens
189 Nrbf2  
Two-hybrid Mus musculus
190 CPE 1363
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here