Gene description for MAP3K1
Gene name mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Gene symbol MAP3K1
Other names/aliases MAPKKK1
Species Homo sapiens
 Database cross references - MAP3K1
ExoCarta ExoCarta_4214
Entrez Gene 4214
HGNC 6848
MIM 600982
UniProt Q13233  
 MAP3K1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for MAP3K1
Molecular Function
    protein kinase binding GO:0019901 IPI
    JUN kinase kinase activity GO:0008545 IEA
    MAP kinase kinase kinase activity GO:0004709 NAS
    protein kinase activity GO:0004672 IDA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    regulation of mitotic cell cycle GO:0007346 IBA
    wound healing GO:0042060 IEA
    regulation of cell migration GO:0030334 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    activation of MAPKK activity GO:0000186 NAS
    activation of protein kinase activity GO:0032147 IBA
    eyelid development in camera-type eye GO:0061029 IEA
    protein phosphorylation GO:0006468 NAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    epithelial cell morphogenesis GO:0003382 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    stress-activated MAPK cascade GO:0051403 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    innate immune response GO:0045087 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    activation of JUN kinase activity GO:0007257 IEA
    positive regulation of actin filament polymerization GO:0030838 IEA
    cellular response to mechanical stimulus GO:0071260 IEP
    apoptotic mitochondrial changes GO:0008637 IEA
    regulation of apoptotic process GO:0042981 IBA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAP3K1 in exosomes
Experiment ID 126
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for MAP3K1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRB2 2885
Invivo Homo sapiens
Invitro Homo sapiens
2 DVL2 1856
Affinity Capture-Western Homo sapiens
3 MAP2K4 6416
Invivo Homo sapiens
4 RPL30 6156
Affinity Capture-MS Homo sapiens
5 PAK3  
Invivo Homo sapiens
6 AXIN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 YWHAE 7531
Reconstituted Complex Homo sapiens
8 CDC37 11140
Affinity Capture-MS Homo sapiens
9 IKBKB 3551
Invivo Homo sapiens
10 TRAF2  
Invivo Homo sapiens
11 MAP4K4 9448
Affinity Capture-Western Homo sapiens
12 RPL4 6124
Affinity Capture-MS Homo sapiens
13 FKBP5 2289
Affinity Capture-MS Homo sapiens
14 UBE2I 7329
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
15 MAPK8IP3  
Invitro Homo sapiens
16 MAP4K2  
Reconstituted Complex Homo sapiens
17 RPS13 6207
Affinity Capture-MS Homo sapiens
18 DCAF7 10238
Affinity Capture-MS Homo sapiens
19 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
20 RAF1 5894
Invivo Homo sapiens
Invitro Homo sapiens
21 MAPK8 5599
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 CHUK 1147
Invivo Homo sapiens
23 MAP3K10  
Invivo Homo sapiens
24 ECSIT 51295
Invivo Homo sapiens
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