Gene description for DNAJA1
Gene name DnaJ (Hsp40) homolog, subfamily A, member 1
Gene symbol DNAJA1
Other names/aliases DJ-2
DjA1
HDJ2
HSDJ
HSJ2
HSPF4
NEDD7
hDJ-2
Species Homo sapiens
 Database cross references - DNAJA1
ExoCarta ExoCarta_3301
Vesiclepedia VP_3301
Entrez Gene 3301
HGNC 5229
MIM 602837
UniProt P31689  
 DNAJA1 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 22635005    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for DNAJA1
Molecular Function
    G protein-coupled receptor binding GO:0001664 IPI
    ATPase activator activity GO:0001671 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    Hsp70 protein binding GO:0030544 IDA
    Tat protein binding GO:0030957 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    metal ion binding GO:0046872 IEA
    low-density lipoprotein particle receptor binding GO:0050750 IDA
    unfolded protein binding GO:0051082 IEA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IDA
    protein-folding chaperone binding GO:0051087 IPI
    C3HC4-type RING finger domain binding GO:0055131 IPI
Biological Process
    protein folding GO:0006457 TAS
    response to unfolded protein GO:0006986 TAS
    spermatogenesis GO:0007283 IEA
    response to heat GO:0009408 IEA
    flagellated sperm motility GO:0030317 IEA
    androgen receptor signaling pathway GO:0030521 IEA
    negative regulation of protein ubiquitination GO:0031397 IDA
    protein refolding GO:0042026 IBA
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of JUN kinase activity GO:0043508 IMP
    regulation of protein transport GO:0051223 IDA
    protein localization to mitochondrion GO:0070585 IMP
    negative regulation of establishment of protein localization to mitochondrion GO:1903748 IDA
    negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway GO:1905259 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    extracellular exosome GO:0070062 HDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 TAS
 Experiment description of studies that identified DNAJA1 in sEVs
1
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
18
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 200
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|TSG101|Alix|GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22635005    
Organism Homo sapiens
Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Authors "Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, Garcia-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D."
Journal name Nat Med
Publication year 2012
Sample Melanoma cells
Sample name B16-F10
SK-MEL-202
SK-MEL035
SK-MEL-265
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
36
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
37
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
40
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
43
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
44
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
46
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
48
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
49
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
52
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
53
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
54
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
55
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
56
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
57
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
58
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DNAJA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC2 3066
Co-fractionation Homo sapiens
2 SHC1 6464
Affinity Capture-MS Homo sapiens
3 LDHB 3945
Co-fractionation Homo sapiens
4 RIN3  
Affinity Capture-MS Homo sapiens
5 DYNC1I2 1781
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
6 ACTC1 70
Affinity Capture-MS Homo sapiens
7 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 UBC 7316
Affinity Capture-MS Homo sapiens
10 CSE1L 1434
Co-fractionation Homo sapiens
11 VHL  
Affinity Capture-MS Homo sapiens
12 SOX2  
Affinity Capture-MS Homo sapiens
13 EFNA3  
Affinity Capture-MS Homo sapiens
14 WDR76  
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
15 OSBPL9 114883
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NUDT3 11165
Two-hybrid Homo sapiens
17 HSPH1 10808
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
18 MARCH5  
Proximity Label-MS Homo sapiens
19 PPEF1  
Affinity Capture-MS Homo sapiens
20 DNAJB1 3337
Affinity Capture-MS Homo sapiens
21 GSPT1 2935
Co-fractionation Homo sapiens
22 HERC2 8924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FAF1 11124
Co-fractionation Homo sapiens
24 METTL23  
Affinity Capture-MS Homo sapiens
25 BCS1L 617
Two-hybrid Homo sapiens
26 PPP6R3 55291
Co-fractionation Homo sapiens
27 GPS1 2873
Co-fractionation Homo sapiens
28 PSMD14 10213
Affinity Capture-MS Homo sapiens
29 DYNLT1 6993
Affinity Capture-MS Homo sapiens
30 NOL11  
Affinity Capture-MS Homo sapiens
31 Mad2l1 56150
Affinity Capture-MS Mus musculus
32 KLF16  
Affinity Capture-MS Homo sapiens
33 XRCC4  
Co-fractionation Homo sapiens
34 CHMP4C 92421
Affinity Capture-MS Homo sapiens
35 MTCH2 23788
Proximity Label-MS Homo sapiens
36 CRY1  
Affinity Capture-MS Homo sapiens
37 USP11 8237
Affinity Capture-MS Homo sapiens
38 MTMR11  
Affinity Capture-MS Homo sapiens
39 MAP1B 4131
Co-fractionation Homo sapiens
40 UBASH3B 84959
Affinity Capture-MS Homo sapiens
41 ESRRB  
Affinity Capture-MS Homo sapiens
42 EEF1E1 9521
Co-fractionation Homo sapiens
43 TUBB 203068
Affinity Capture-MS Homo sapiens
44 AMPD2 271
Co-fractionation Homo sapiens
45 NUDC 10726
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MYH9 4627
Co-fractionation Homo sapiens
47 OGT 8473
Reconstituted Complex Homo sapiens
48 ACADM 34
Co-fractionation Homo sapiens
49 NOS2  
Affinity Capture-MS Homo sapiens
50 FNBP1 23048
Co-fractionation Homo sapiens
51 HECTD1 25831
Affinity Capture-MS Homo sapiens
52 CUL2 8453
Affinity Capture-MS Homo sapiens
53 GLIS3  
Affinity Capture-MS Homo sapiens
54 TMPO 7112
Affinity Capture-MS Homo sapiens
55 HADHB 3032
Co-fractionation Homo sapiens
56 AHCYL1 10768
Co-fractionation Homo sapiens
57 SIRT7  
Affinity Capture-MS Homo sapiens
58 IQCB1  
Affinity Capture-MS Homo sapiens
59 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 FKBP8 23770
Affinity Capture-MS Homo sapiens
61 FRMD5 84978
Affinity Capture-MS Homo sapiens
62 EEF1B2 1933
Co-fractionation Homo sapiens
63 RBBP4 5928
Co-fractionation Homo sapiens
64 STRAP 11171
Co-fractionation Homo sapiens
65 TUBA4A 7277
Co-fractionation Homo sapiens
66 PRMT5 10419
Co-fractionation Homo sapiens
67 RPA3 6119
Proximity Label-MS Homo sapiens
68 LTV1  
Co-fractionation Homo sapiens
69 HSPA1A 3303
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
70 HSPA4 3308
Co-fractionation Homo sapiens
71 PSMC5 5705
Co-fractionation Homo sapiens
72 ILK 3611
Affinity Capture-MS Homo sapiens
73 NFATC2  
Affinity Capture-MS Homo sapiens
74 STUB1 10273
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
75 AATK  
Affinity Capture-MS Homo sapiens
76 Tubg1 103733
Affinity Capture-MS Mus musculus
77 Tubb5 22154
Affinity Capture-MS Mus musculus
78 RBM25 58517
Co-fractionation Homo sapiens
79 RUVBL2 10856
Co-fractionation Homo sapiens
80 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
81 TRA2B 6434
Co-fractionation Homo sapiens
82 TARS 6897
Cross-Linking-MS (XL-MS) Homo sapiens
83 H2AFY 9555
Co-fractionation Homo sapiens
84 FAR1 84188
Affinity Capture-MS Homo sapiens
85 RNASEH2A 10535
Affinity Capture-MS Homo sapiens
86 LARP7 51574
Affinity Capture-MS Homo sapiens
87 SNRNP70 6625
Co-fractionation Homo sapiens
88 CLTA 1211
Proximity Label-MS Homo sapiens
89 AIMP1 9255
Co-fractionation Homo sapiens
90 CUL7 9820
Affinity Capture-MS Homo sapiens
91 ADRM1 11047
Co-fractionation Homo sapiens
92 HSPA5 3309
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
93 HSPA1B 3304
Affinity Capture-MS Homo sapiens
94 EDRF1  
Proximity Label-MS Homo sapiens
95 HBB 3043
Affinity Capture-MS Homo sapiens
96 SPRTN  
Affinity Capture-MS Homo sapiens
97 MCM2 4171
Affinity Capture-MS Homo sapiens
98 SEP15 9403
Affinity Capture-MS Homo sapiens
99 HSPA6 3310
Affinity Capture-Western Homo sapiens
100 EIF2S3 1968
Co-fractionation Homo sapiens
101 BTF3 689
Affinity Capture-MS Homo sapiens
102 NUDCD3 23386
Proximity Label-MS Homo sapiens
103 CCT6B 10693
Co-fractionation Homo sapiens
104 NDUFA4 4697
Affinity Capture-MS Homo sapiens
105 SAFB 6294
Co-fractionation Homo sapiens
106 B3GNT2 10678
Affinity Capture-MS Homo sapiens
107 OGDH 4967
Co-fractionation Homo sapiens
108 PACRG 135138
Affinity Capture-Western Homo sapiens
109 USO1 8615
Co-fractionation Homo sapiens
110 HSPA2 3306
Affinity Capture-MS Homo sapiens
111 MRPS23 51649
Co-fractionation Homo sapiens
112 SRRM2 23524
Affinity Capture-MS Homo sapiens
113 RECQL4  
Affinity Capture-MS Homo sapiens
114 OBSL1 23363
Affinity Capture-MS Homo sapiens
115 STIP1 10963
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
116 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
117 CUL1 8454
Affinity Capture-MS Homo sapiens
118 PSAP 5660
Co-fractionation Homo sapiens
119 EBAG9 9166
Proximity Label-MS Homo sapiens
120 Tuba3a 22144
Affinity Capture-MS Mus musculus
121 SMC2 10592
Co-fractionation Homo sapiens
122 LSM2 57819
Two-hybrid Homo sapiens
123 CCT2 10576
Co-fractionation Homo sapiens
124 JMJD6 23210
Affinity Capture-MS Homo sapiens
125 HS2ST1 9653
Affinity Capture-MS Homo sapiens
126 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
127 WWP2 11060
Affinity Capture-MS Homo sapiens
128 EIF2A 83939
Affinity Capture-MS Homo sapiens
129 WRAP73 49856
Affinity Capture-MS Homo sapiens
130 EXOC4 60412
Affinity Capture-MS Homo sapiens
131 TUBB2B 347733
Affinity Capture-MS Homo sapiens
132 TUBA1C 84790
Affinity Capture-MS Homo sapiens
133 NELFE 7936
Affinity Capture-MS Homo sapiens
134 LUC7L2 51631
Co-fractionation Homo sapiens
135 UQCRC1 7384
Affinity Capture-MS Homo sapiens
136 SPDYE4  
Affinity Capture-MS Homo sapiens
137 RPLP0 6175
Co-fractionation Homo sapiens
138 PPM1G 5496
Co-fractionation Homo sapiens
139 Ksr1  
Affinity Capture-MS Mus musculus
140 CYLD  
Affinity Capture-MS Homo sapiens
141 KDF1  
Affinity Capture-MS Homo sapiens
142 FBXL4 26235
Affinity Capture-MS Homo sapiens
143 PSMA3 5684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 PPP4R2  
Co-fractionation Homo sapiens
145 CANX 821
Co-fractionation Homo sapiens
146 USP15 9958
Co-fractionation Homo sapiens
147 RC3H2  
Affinity Capture-MS Homo sapiens
148 CAV2 858
Two-hybrid Homo sapiens
149 TUBB4B 10383
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
150 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 KIAA1524 57650
Proximity Label-MS Homo sapiens
152 RIPK4  
Affinity Capture-MS Homo sapiens
153 MYC  
Affinity Capture-MS Homo sapiens
154 SART3 9733
Affinity Capture-MS Homo sapiens
155 DDB1 1642
Co-fractionation Homo sapiens
156 SNW1 22938
Affinity Capture-MS Homo sapiens
157 DDRGK1 65992
Affinity Capture-MS Homo sapiens
158 XRCC3  
Affinity Capture-MS Homo sapiens
159 CLGN 1047
Co-fractionation Homo sapiens
160 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 EIF3H 8667
Affinity Capture-MS Homo sapiens
162 Bax 12028
Affinity Capture-Western Mus musculus
163 CLPP 8192
Proximity Label-MS Homo sapiens
164 PRKCSH 5589
Co-fractionation Homo sapiens
165 HDGF 3068
Affinity Capture-MS Homo sapiens
166 H3F3A 3020
Co-fractionation Homo sapiens
167 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
168 FOLR1 2348
Affinity Capture-MS Homo sapiens
169 PGR  
Reconstituted Complex Homo sapiens
170 HDAC6 10013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 PPP2R2C 5522
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
172 OTUB1 55611
Affinity Capture-MS Homo sapiens
173 MAPK6  
Affinity Capture-MS Homo sapiens
174 NCAPD2 9918
Co-fractionation Homo sapiens
175 BAG5 9529
Affinity Capture-MS Homo sapiens
176 C16orf72 29035
Affinity Capture-MS Homo sapiens
177 CBWD3  
Affinity Capture-MS Homo sapiens
178 BRD1 23774
Affinity Capture-MS Homo sapiens
179 HSPA4L 22824
Co-fractionation Homo sapiens
180 PTRF 284119
Co-fractionation Homo sapiens
181 RCOR1  
Co-fractionation Homo sapiens
182 KCNQ4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
183 MECP2 4204
Affinity Capture-MS Homo sapiens
184 MRPS27 23107
Co-fractionation Homo sapiens
185 UBE2H 7328
Affinity Capture-MS Homo sapiens
186 HNRNPC 3183
Co-fractionation Homo sapiens
187 EEF1G 1937
Co-fractionation Homo sapiens
188 TPP1 1200
Co-fractionation Homo sapiens
189 PFKP 5214
Co-fractionation Homo sapiens
190 YWHAE 7531
Affinity Capture-MS Homo sapiens
191 DNAJA4 55466
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 RPS19 6223
Co-fractionation Homo sapiens
193 RANBP3 8498
Affinity Capture-MS Homo sapiens
194 PSMD10 5716
Co-fractionation Homo sapiens
195 RPS6KB2  
Affinity Capture-MS Homo sapiens
196 Tubb4b 227613
Affinity Capture-MS Mus musculus
197 LRPPRC 10128
Co-fractionation Homo sapiens
198 CAPZB 832
Affinity Capture-MS Homo sapiens
199 RUVBL1 8607
Co-fractionation Homo sapiens
200 CDC16 8881
Two-hybrid Homo sapiens
201 VARS 7407
Co-fractionation Homo sapiens
202 SPAG9 9043
Co-fractionation Homo sapiens
203 IQGAP1 8826
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
204 PSMC2 5701
Co-fractionation Homo sapiens
205 PLD2 5338
Affinity Capture-MS Homo sapiens
206 ATG16L1 55054
Affinity Capture-MS Homo sapiens
207 GOLGA4  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
208 UQCRQ 27089
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
209 HSPA8 3312
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
210 HSPBP1 23640
Proximity Label-MS Homo sapiens
211 NF2 4771
Affinity Capture-MS Homo sapiens
212 DLD 1738
Affinity Capture-MS Homo sapiens
213 ATP5A1 498
Cross-Linking-MS (XL-MS) Homo sapiens
214 EML4 27436
Co-fractionation Homo sapiens
215 ASNA1 439
Co-fractionation Homo sapiens
216 IRAK1  
Affinity Capture-MS Homo sapiens
217 CUL4B 8450
Affinity Capture-MS Homo sapiens
218 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
219 PHAX  
Co-fractionation Homo sapiens
220 SMEK1 55671
Co-fractionation Homo sapiens
221 TUBA1A 7846
Affinity Capture-MS Homo sapiens
222 PANK4 55229
Proximity Label-MS Homo sapiens
223 KPNA4 3840
Co-fractionation Homo sapiens
224 ACACA 31
Positive Genetic Homo sapiens
225 DNAJC5 80331
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
226 MAPT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
227 TUBA1B 10376
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
228 TNPO2 30000
Co-fractionation Homo sapiens
229 NTRK1 4914
Affinity Capture-MS Homo sapiens
230 U2AF2 11338
Co-fractionation Homo sapiens
231 PPA2 27068
Affinity Capture-MS Homo sapiens
232 HMGCL 3155
Two-hybrid Homo sapiens
233 COPS3 8533
Co-fractionation Homo sapiens
234 LRRFIP1 9208
Co-fractionation Homo sapiens
235 NFKB2 4791
Co-fractionation Homo sapiens
236 HDAC5 10014
Affinity Capture-MS Homo sapiens
237 DNM1L 10059
Affinity Capture-MS Homo sapiens
238 BZW1 9689
Co-fractionation Homo sapiens
239 ENDOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
240 IFT122 55764
Affinity Capture-MS Homo sapiens
241 ASB5  
Affinity Capture-MS Homo sapiens
242 MIER1  
Co-fractionation Homo sapiens
243 ZBTB2 57621
Affinity Capture-MS Homo sapiens
244 DCXR 51181
Affinity Capture-MS Homo sapiens
245 METTL14  
Affinity Capture-MS Homo sapiens
246 DNAJB12 54788
Affinity Capture-MS Homo sapiens
247 BAG2 9532
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
248 OSGEP 55644
Affinity Capture-MS Homo sapiens
249 FBXO6 26270
Affinity Capture-MS Homo sapiens
250 METTL21B  
Affinity Capture-MS Homo sapiens
251 OSBPL11 114885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
252 NCAPH 23397
Co-fractionation Homo sapiens
253 ARL4D  
Two-hybrid Homo sapiens
254 NLRP1  
Affinity Capture-MS Homo sapiens
255 RAF1 5894
Affinity Capture-MS Homo sapiens
256 HYOU1 10525
Co-fractionation Homo sapiens
257 FLII 2314
Co-fractionation Homo sapiens
258 HAX1  
Proximity Label-MS Homo sapiens
259 Pias1  
Two-hybrid Mus musculus
260 HSPA9 3313
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
261 TSR1 55720
Co-fractionation Homo sapiens
262 ARAF 369
Affinity Capture-MS Homo sapiens
263 AKAP1 8165
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
264 NCAPG 64151
Co-fractionation Homo sapiens
265 HIF1A 3091
Affinity Capture-MS Homo sapiens
266 AHSA1 10598
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
267 SUGT1 10910
Affinity Capture-MS Homo sapiens
268 LLGL2 3993
Affinity Capture-MS Homo sapiens
269 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
270 FBXL6  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
271 PDHA1 5160
Affinity Capture-MS Homo sapiens
272 THRAP3 9967
Co-fractionation Homo sapiens
273 PTTG1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
274 UFL1 23376
Affinity Capture-MS Homo sapiens
275 KDM1A 23028
Co-fractionation Homo sapiens
276 MED12  
Affinity Capture-MS Homo sapiens
277 LRRK2 120892
Affinity Capture-MS Homo sapiens
278 METTL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
279 ERG  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
280 EID1  
Affinity Capture-MS Homo sapiens
281 RC3H1 149041
Affinity Capture-MS Homo sapiens
282 ATXN3 4287
Affinity Capture-MS Homo sapiens
283 LUC7L3 51747
Co-fractionation Homo sapiens
284 TM4SF1 4071
Two-hybrid Homo sapiens
285 CUL5 8065
Affinity Capture-MS Homo sapiens
286 CRBN  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
287 HKDC1 80201
Affinity Capture-MS Homo sapiens
288 SLFN11 91607
Affinity Capture-MS Homo sapiens
289 CCT3 7203
Co-fractionation Homo sapiens
290 QARS 5859
Co-fractionation Homo sapiens
291 PAXIP1  
Two-hybrid Homo sapiens
292 RPS3A 6189
Co-fractionation Homo sapiens
293 TNPO1 3842
Co-fractionation Homo sapiens
294 PSMD11 5717
Co-fractionation Homo sapiens
295 FBXO25  
Affinity Capture-MS Homo sapiens
296 HSPA1L 3305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
297 TROVE2 6738
Co-fractionation Homo sapiens
298 DNAJB3 414061
Proximity Label-MS Homo sapiens
299 SNRPD2 6633
Co-fractionation Homo sapiens
300 CD58 965
Two-hybrid Homo sapiens
301 USP36  
Affinity Capture-MS Homo sapiens
302 KIAA1429 25962
Affinity Capture-MS Homo sapiens
303 PPP1R12A 4659
Co-fractionation Homo sapiens
304 TGFB1 7040
Affinity Capture-MS Homo sapiens
305 PCK1 5105
Affinity Capture-MS Homo sapiens
306 COPS5 10987
Affinity Capture-MS Homo sapiens
307 FBXW7  
Affinity Capture-MS Homo sapiens
308 AGO3  
Affinity Capture-MS Homo sapiens
309 PSMC6 5706
Co-fractionation Homo sapiens
310 ACTR1A 10121
Co-fractionation Homo sapiens
311 CDC73  
Affinity Capture-MS Homo sapiens
312 ATP5H 10476
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
313 MYCN  
Affinity Capture-MS Homo sapiens
314 CTCF  
Co-fractionation Homo sapiens
315 LAMTOR1 55004
Proximity Label-MS Homo sapiens
316 Tuba1b 22143
Affinity Capture-MS Mus musculus
317 ERBB3 2065
Affinity Capture-MS Homo sapiens
318 HIVEP1 3096
Two-hybrid Homo sapiens
319 HTT 3064
Affinity Capture-MS Homo sapiens
320 ANKHD1 54882
Co-fractionation Homo sapiens
321 VCP 7415
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
322 PCSK9 255738
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
323 MYH10 4628
Co-fractionation Homo sapiens
324 KIAA1033 23325
Co-fractionation Homo sapiens
325 ST13 6767
Proximity Label-MS Homo sapiens
326 GOLGA3 2802
Co-fractionation Homo sapiens
327 COPS2 9318
Co-fractionation Homo sapiens
328 OTUD1 220213
Affinity Capture-MS Homo sapiens
329 PSMD13 5719
Affinity Capture-MS Homo sapiens
330 NELFCD 51497
Two-hybrid Homo sapiens
331 CCT6A 908
Co-fractionation Homo sapiens
332 PDIA3 2923
Co-fractionation Homo sapiens
333 CCNJL  
Affinity Capture-MS Homo sapiens
334 SUZ12  
Affinity Capture-MS Homo sapiens
335 PRKACA 5566
Affinity Capture-MS Homo sapiens
336 AGO4  
Affinity Capture-MS Homo sapiens
337 CLTC 1213
Proximity Label-MS Homo sapiens
338 GPR37  
Affinity Capture-Western Homo sapiens
339 RNF207  
Affinity Capture-Western Homo sapiens
340 THADA 63892
Proximity Label-MS Homo sapiens
341 BMP4 652
Affinity Capture-MS Homo sapiens
342 KCNH2  
Affinity Capture-MS Homo sapiens
343 SPTAN1 6709
Co-fractionation Homo sapiens
344 SRRM1 10250
Affinity Capture-MS Homo sapiens
345 Fbl 14113
Affinity Capture-MS Mus musculus
346 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
347 USP35  
Affinity Capture-MS Homo sapiens
348 SMARCC1 6599
Co-fractionation Homo sapiens
349 NT5C2 22978
Co-fractionation Homo sapiens
350 HBA2 3040
Affinity Capture-MS Homo sapiens
351 SMURF1 57154
Affinity Capture-MS Homo sapiens
352 HDAC1 3065
Co-fractionation Homo sapiens
353 CHD4 1108
Co-fractionation Homo sapiens
354 LRRK1 79705
Affinity Capture-MS Homo sapiens
355 CAD 790
Co-fractionation Homo sapiens
356 RARS 5917
Co-fractionation Homo sapiens
357 C9orf72  
Affinity Capture-MS Homo sapiens
358 CFTR 1080
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
359 LIMK2 3985
Affinity Capture-MS Homo sapiens
360 CDC5L 988
Affinity Capture-MS Homo sapiens
361 RBBP7 5931
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here