Gene description for HBG2
Gene name hemoglobin, gamma G
Gene symbol HBG2
Other names/aliases HBG-T1
TNCY
Species Homo sapiens
 Database cross references - HBG2
ExoCarta ExoCarta_3048
Vesiclepedia VP_3048
Entrez Gene 3048
HGNC 4832
MIM 142250
UniProt P69892  
 HBG2 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for HBG2
Molecular Function
    peroxidase activity GO:0004601 IBA
    oxygen carrier activity GO:0005344 IBA
    oxygen carrier activity GO:0005344 IDA
    protein binding GO:0005515 IPI
    oxygen binding GO:0019825 IBA
    heme binding GO:0020037 IBA
    haptoglobin binding GO:0031720 IBA
    hemoglobin alpha binding GO:0031721 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    carbon dioxide transport GO:0015670 NAS
    oxygen transport GO:0015671 IDA
    oxygen transport GO:0015671 NAS
    hydrogen peroxide catabolic process GO:0042744 IBA
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    hemoglobin complex GO:0005833 IBA
    hemoglobin complex GO:0005833 IPI
    haptoglobin-hemoglobin complex GO:0031838 IBA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified HBG2 in sEVs
1
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HBG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 GNAZ 2781
Affinity Capture-MS Homo sapiens
3 RPSA 3921
Two-hybrid Homo sapiens
4 SMAD5 4090
Two-hybrid Homo sapiens
5 HBG2 3048
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
6 CADM4 199731
Affinity Capture-MS Homo sapiens
7 HBA2 3040
Two-hybrid Homo sapiens
8 HRAS 3265
Affinity Capture-MS Homo sapiens
9 ERBB3 2065
Affinity Capture-MS Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 UBB 7314
Affinity Capture-MS Homo sapiens
12 TTC19  
Affinity Capture-MS Homo sapiens
13 RAP1BL 643752
Affinity Capture-MS Homo sapiens
14 TRIM21 6737
Affinity Capture-MS Homo sapiens
15 MCM2 4171
Affinity Capture-MS Homo sapiens
16 HBM  
Two-hybrid Homo sapiens
17 SRC 6714
Affinity Capture-MS Homo sapiens
18 MICAL1 64780
Affinity Capture-MS Homo sapiens
19 CCNY 219771
Affinity Capture-MS Homo sapiens
20 HBA1 3039
Two-hybrid Homo sapiens
21 XPNPEP1 7511
Two-hybrid Homo sapiens
22 ITGA4 3676
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HBG2 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome
Hemostasis TAS Reactome





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