Gene description for RANBP2
Gene name RAN binding protein 2
Gene symbol RANBP2
Other names/aliases ADANE
ANE1
IIAE3
NUP358
TRP1
TRP2
Species Homo sapiens
 Database cross references - RANBP2
ExoCarta ExoCarta_5903
Entrez Gene 5903
HGNC 9848
MIM 601181
UniProt P49792  
 RANBP2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RANBP2
Molecular Function
    Ran GTPase binding GO:0008536 TAS
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IEA
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    RNA binding GO:0003723 IEA
Biological Process
    post-translational protein modification GO:0043687 TAS
    gene expression GO:0010467 TAS
    cellular protein metabolic process GO:0044267 TAS
    protein sumoylation GO:0016925 TAS
    protein folding GO:0006457 IEA
    negative regulation of glucokinase activity GO:0033132 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    hexose transport GO:0008645 TAS
    transmembrane transport GO:0055085 TAS
    glucose transport GO:0015758 TAS
    viral transcription GO:0019083 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    viral life cycle GO:0019058 TAS
    carbohydrate metabolic process GO:0005975 TAS
    regulation of gluconeogenesis involved in cellular glucose homeostasis GO:0090526 IEA
    regulation of cellular response to heat GO:1900034 TAS
    cellular response to heat GO:0034605 TAS
    regulation of glucose transport GO:0010827 TAS
    small molecule metabolic process GO:0044281 TAS
    protein peptidyl-prolyl isomerization GO:0000413 IEA
    mRNA transport GO:0051028 IEA
    mitotic cell cycle GO:0000278 TAS
    mitotic nuclear envelope disassembly GO:0007077 TAS
    protein import into nucleus GO:0006606 TAS
    viral process GO:0016032 TAS
Subcellular Localization
    membrane GO:0016020 IDA
    nuclear pore nuclear basket GO:0044615 IDA
    nuclear inclusion body GO:0042405 IDA
    cytosol GO:0005829 TAS
    nuclear membrane GO:0031965 IDA
    mitochondrion GO:0005739 IEA
    nuclear pore GO:0005643 IDA
 Experiment description of studies that identified RANBP2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RANBP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XPOT 11260
Invitro Homo sapiens
2 KPNB1 3837
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 OPN1MW  
Invivo Homo sapiens
4 UBE2I 7329
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
5 IPO5 3843
Invitro Homo sapiens
Invivo Homo sapiens
6 RAN 5901
Reconstituted Complex Homo sapiens
7 KPNA1 3836
Reconstituted Complex Homo sapiens
8 RANGAP1 5905
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
9 TNPO1 3842
Invitro Homo sapiens
10 OPN1LW  
Invivo Homo sapiens
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