ExoCarta: Gene summary

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Gene description for SEC11A
Gene name	SEC11 homolog A (S. cerevisiae)
Gene symbol	SEC11A
Other names/aliases	1810012E07Rik SEC11L1 SPC18 SPCS4A sid2895
Species	Homo sapiens
Database cross references - SEC11A
ExoCarta	ExoCarta_23478
Vesiclepedia	VP_23478
Entrez Gene	23478
HGNC	17718
UniProt	P67812
SEC11A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Platelets	25332113
Platelets	25332113
Platelets	25332113

Gene ontology annotations for SEC11A

Molecular Function
	serine-type endopeptidase activity	GO:0004252	IDA
	serine-type endopeptidase activity	GO:0004252	TAS
	protein binding	GO:0005515	IPI
	peptidase activity	GO:0008233	IBA
Biological Process
	signal peptide processing	GO:0006465	IBA
	signal peptide processing	GO:0006465	IDA
	response to virus	GO:0009615	TAS
Subcellular Localization
	signal peptidase complex	GO:0005787	IBA
	signal peptidase complex	GO:0005787	IDA
	signal peptidase complex	GO:0005787	IPI
	endoplasmic reticulum membrane	GO:0005789	IDA
	endoplasmic reticulum membrane	GO:0005789	TAS

Experiment description of studies that identified SEC11A in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

231

MISEV standards
✘	Biophysical techniques
✔ Alix\|CD63\|CD9	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25332113

Organism

Homo sapiens

Experiment description

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Authors

"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."

Journal name

Transfusion

Publication year

2015

Sample

Platelets

Sample name

PL-Exs - Rep 1

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient

Flotation density

1.12-1.15 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

232

MISEV standards
✘	Biophysical techniques
✘	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25332113

Organism

Homo sapiens

Experiment description

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Authors

"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."

Journal name

Transfusion

Publication year

2015

Sample

Platelets

Sample name

PL-Exs - Rep 2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient

Flotation density

1.12-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

233

MISEV standards
✘	Biophysical techniques
✘	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25332113

Organism

Homo sapiens

Experiment description

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Authors

"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."

Journal name

Transfusion

Publication year

2015

Sample

Platelets

Sample name

PL-Exs - Rep 3

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient

Flotation density

1.12-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for SEC11A

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

UBE2H

7328

Affinity Capture-MS

Homo sapiens

SSMEM1

Affinity Capture-MS

Homo sapiens

ADAM30

11085

Affinity Capture-MS

Homo sapiens

CANX

821

Affinity Capture-MS

Homo sapiens

LYPD6B

130576

Affinity Capture-MS

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

PRTN3

5657

Affinity Capture-MS

Homo sapiens

LYPD3

27076

Affinity Capture-MS

Homo sapiens

SPCS3

60559

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TSPAN5

10098

Affinity Capture-MS

Homo sapiens

CSNK2B

1460

Two-hybrid

Homo sapiens

BCHE

590

Two-hybrid

Homo sapiens

CTAGE5

4253

Affinity Capture-MS

Homo sapiens

TTYH1

Affinity Capture-MS

Homo sapiens

GABRE

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

VAPA

9218

Affinity Capture-MS

Homo sapiens

LRRC59

55379

Proximity Label-MS

Homo sapiens

PDIA4

9601

Proximity Label-MS

Homo sapiens

DNAJC2

27000

Affinity Capture-MS

Homo sapiens

P2RX1

5023

Affinity Capture-MS

Homo sapiens

ADAM33

80332

Two-hybrid

Homo sapiens

GOLGA7

51125

Affinity Capture-MS

Homo sapiens

GPC1

2817

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PLAUR

5329

Affinity Capture-MS

Homo sapiens

Spcs2

66624

Affinity Capture-MS

Mus musculus

FBXL14

Affinity Capture-MS

Homo sapiens

KLRK1

Affinity Capture-MS

Homo sapiens

NR3C1

2908

Proximity Label-MS

Homo sapiens

LOC254896

Affinity Capture-MS

Homo sapiens

RB1CC1

9821

Affinity Capture-MS

Homo sapiens

WNT6

Affinity Capture-MS

Homo sapiens

FBXO6

26270

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MIA3

375056

Affinity Capture-MS

Homo sapiens

AMHR2

Affinity Capture-MS

Homo sapiens

SLC35F6

54978

Two-hybrid

Homo sapiens

TM2D3

Affinity Capture-MS

Homo sapiens

NUPR1

Two-hybrid

Homo sapiens

IDS

Affinity Capture-MS

Homo sapiens

SPCS1

28972

Affinity Capture-MS

Homo sapiens

FBXO2

26232

Affinity Capture-MS

Homo sapiens

MCM2

4171

Affinity Capture-MS

Homo sapiens

TREM1

54210

Affinity Capture-MS

Homo sapiens

ASIC1

Affinity Capture-MS

Homo sapiens

CIT

11113

Affinity Capture-MS

Homo sapiens

UPK1A

11045

Affinity Capture-MS

Homo sapiens

NCLN

56926

Affinity Capture-MS

Homo sapiens

PVRIG

Affinity Capture-MS

Homo sapiens

DNASE1L1

1774

Affinity Capture-MS

Homo sapiens

RNF13

11342

Affinity Capture-MS

Homo sapiens

VDAC1

7416

Cross-Linking-MS (XL-MS)

Homo sapiens

RTN4R

65078

Affinity Capture-MS

Homo sapiens

RNF2

Affinity Capture-MS

Homo sapiens

CCR1

Affinity Capture-MS

Homo sapiens

TNFRSF14

Affinity Capture-MS

Homo sapiens

SEC11B

Cross-Linking-MS (XL-MS)

Homo sapiens

C9orf72

Affinity Capture-MS

Homo sapiens

SPCS2

9789

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ST3GAL3

Affinity Capture-MS

Homo sapiens

ZACN

Affinity Capture-MS

Homo sapiens

CCDC47

57003

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which SEC11A is involved

Pathway	Evidence	Source
Disease	TAS	Reactome
Incretin synthesis, secretion, and inactivation	IEA	Reactome
Infectious disease	TAS	Reactome
Maturation of hRSV A proteins	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Metabolism of proteins	IEA	Reactome
Peptide hormone metabolism	TAS	Reactome
Peptide hormone metabolism	IEA	Reactome
Respiratory syncytial virus (RSV) genome replication, transcription and translation	TAS	Reactome
Respiratory Syncytial Virus Infection Pathway	TAS	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome
Synthesis, secretion, and deacylation of Ghrelin	TAS	Reactome
Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1)	IEA	Reactome
Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP)	IEA	Reactome
Translation	IEA	Reactome
Viral Infection Pathways	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here