Gene description for NEB
Gene name
nebulin
Gene symbol
NEB
Other names/aliases
NEB177D NEM2
Species
Homo sapiens
Database cross references - NEB
ExoCarta
ExoCarta_4703
Entrez Gene
4703
HGNC
7720
MIM
161650
UniProt
P20929
NEB identified in exosomes derived from the following tissue/cell type
Hepatocytes
26054723
Malignant pleural effusions
14975938
Urine
19056867
Gene ontology annotations for NEB
Experiment description of studies that identified NEB in exosomes
1
Experiment ID
237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
2
Experiment ID
51
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
14975938
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors
Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name
AJRCMB
Publication year
2004
Sample
Malignant pleural effusions
Sample name
Pleural Fluid - Breast cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
3
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for NEB
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
MYPN
84665
Reconstituted Complex
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
2
ACTN4
81
Invitro
Homo sapiens
3
DES
1674
Two-hybrid
Homo sapiens
4
TTN
7273
Invivo
Homo sapiens
Invivo
Homo sapiens
5
TMOD4
Reconstituted Complex
Homo sapiens
6
TMOD1
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which NEB is involved