Gene description for TTN
Gene name titin
Gene symbol TTN
Other names/aliases CMD1G
CMH9
CMPD4
EOMFC
HMERF
LGMD2J
MYLK5
TMD
Species Homo sapiens
 Database cross references - TTN
ExoCarta ExoCarta_7273
Vesiclepedia VP_7273
Entrez Gene 7273
HGNC 12403
MIM 188840
UniProt Q8WZ42  
 TTN identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Plasma 19028452    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for TTN
Molecular Function
    protease binding GO:0002020 IPI
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein tyrosine kinase activity GO:0004713 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IPI
    calmodulin binding GO:0005516 TAS
    ATP binding GO:0005524 IEA
    structural constituent of muscle GO:0008307 IMP
    structural constituent of muscle GO:0008307 TAS
    protein kinase regulator activity GO:0019887 IMP
    enzyme binding GO:0019899 IPI
    protein kinase binding GO:0019901 IPI
    telethonin binding GO:0031433 IPI
    telethonin binding GO:0031433 ISS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    actinin binding GO:0042805 IDA
    actinin binding GO:0042805 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    actin filament binding GO:0051015 IDA
    muscle alpha-actinin binding GO:0051371 IPI
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    structural molecule activity conferring elasticity GO:0097493 IDA
    structural molecule activity conferring elasticity GO:0097493 TAS
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    cardiac muscle hypertrophy GO:0003300 IMP
    chromatin remodeling GO:0006338 IEA
    muscle contraction GO:0006936 NAS
    muscle contraction GO:0006936 TAS
    striated muscle contraction GO:0006941 TAS
    mitotic chromosome condensation GO:0007076 IEP
    positive regulation of gene expression GO:0010628 IGI
    positive regulation of gene expression GO:0010628 IMP
    protein kinase A signaling GO:0010737 IMP
    skeletal muscle thin filament assembly GO:0030240 IMP
    skeletal muscle myosin thick filament assembly GO:0030241 IMP
    detection of muscle stretch GO:0035995 IDA
    detection of muscle stretch GO:0035995 TAS
    sarcomere organization GO:0045214 IBA
    sarcomere organization GO:0045214 IMP
    sarcomerogenesis GO:0048769 IMP
    positive regulation of protein secretion GO:0050714 IGI
    positive regulation of protein secretion GO:0050714 IMP
    response to calcium ion GO:0051592 IDA
    cardiac myofibril assembly GO:0055003 IMP
    cardiac muscle tissue morphogenesis GO:0055008 IMP
    cardiac muscle cell development GO:0055013 IMP
    cardiac muscle contraction GO:0060048 IMP
Subcellular Localization
    condensed nuclear chromosome GO:0000794 IDA
    extracellular region GO:0005576 TAS
    cytosol GO:0005829 TAS
    striated muscle thin filament GO:0005865 IDA
    Z disc GO:0030018 IDA
    M band GO:0031430 IBA
    M band GO:0031430 IDA
    I band GO:0031674 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TTN in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
13
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
16
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
17
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
18
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
21
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TTN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RPS12 6206
Cross-Linking-MS (XL-MS) Homo sapiens
3 SRPK2 6733
Biochemical Activity Homo sapiens
4 SP1  
Affinity Capture-MS Homo sapiens
5 MYPN 84665
Two-hybrid Homo sapiens
6 RPL13A 23521
Cross-Linking-MS (XL-MS) Homo sapiens
7 ADRB2  
Affinity Capture-MS Homo sapiens
8 ALB 213
Affinity Capture-MS Homo sapiens
9 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
10 ANK1 286
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 OBSCN 84033
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CHEK2  
Synthetic Lethality Homo sapiens
14 TRIM55  
Two-hybrid Homo sapiens
15 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
16 SRSF2 6427
Co-fractionation Homo sapiens
17 NEFM 4741
Cross-Linking-MS (XL-MS) Homo sapiens
18 SBDS 51119
Cross-Linking-MS (XL-MS) Homo sapiens
19 TOP2A 7153
Synthetic Lethality Homo sapiens
20 RPL10A 4736
Cross-Linking-MS (XL-MS) Homo sapiens
21 HIST1H1A 3024
Cross-Linking-MS (XL-MS) Homo sapiens
22 PGK1 5230
Cross-Linking-MS (XL-MS) Homo sapiens
23 MLLT4 4301
Cross-Linking-MS (XL-MS) Homo sapiens
24 RBM3 5935
Affinity Capture-MS Homo sapiens
25 MCM2 4171
Affinity Capture-MS Homo sapiens
26 TSC22D2 9819
Two-hybrid Homo sapiens
27 ACTR3 10096
Cross-Linking-MS (XL-MS) Homo sapiens
28 ANKRD2  
Reconstituted Complex Homo sapiens
29 COPS5 10987
Affinity Capture-MS Homo sapiens
30 TRDN 10345
Cross-Linking-MS (XL-MS) Homo sapiens
31 TULP3 7289
Affinity Capture-MS Homo sapiens
32 KMT2A  
Cross-Linking-MS (XL-MS) Homo sapiens
33 CEP57  
Affinity Capture-MS Homo sapiens
34 ADAM2  
Affinity Capture-MS Homo sapiens
35 MYCN  
Affinity Capture-MS Homo sapiens
36 CAPN3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 PABPC4 8761
Cross-Linking-MS (XL-MS) Homo sapiens
38 EEF1A1 1915
Cross-Linking-MS (XL-MS) Homo sapiens
39 DVL2 1856
Affinity Capture-MS Homo sapiens
40 HSPA8 3312
Cross-Linking-MS (XL-MS) Homo sapiens
41 MAPK1 5594
Biochemical Activity Homo sapiens
Synthetic Lethality Homo sapiens
42 PFN1 5216
Cross-Linking-MS (XL-MS) Homo sapiens
43 CHMP4C 92421
Affinity Capture-MS Homo sapiens
44 ANXA7 310
Co-fractionation Homo sapiens
45 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
46 TSPAN33 340348
Affinity Capture-MS Homo sapiens
47 CUL4B 8450
Affinity Capture-MS Homo sapiens
48 SNAP91 9892
Cross-Linking-MS (XL-MS) Homo sapiens
49 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
50 NBR1 4077
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
51 TUBA1A 7846
Synthetic Lethality Homo sapiens
52 RSPH6A  
Affinity Capture-MS Homo sapiens
53 ANKRD1 27063
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
54 PABPC1 26986
Cross-Linking-MS (XL-MS) Homo sapiens
55 CDH1 999
Proximity Label-MS Homo sapiens
56 TNRC18  
Cross-Linking-MS (XL-MS) Homo sapiens
57 RAN 5901
Cross-Linking-MS (XL-MS) Homo sapiens
58 NTRK1 4914
Affinity Capture-MS Homo sapiens
59 Pparg  
Affinity Capture-MS Mus musculus
60 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
61 CHEK1  
Synthetic Lethality Homo sapiens
62 DCTN1 1639
Proximity Label-MS Homo sapiens
63 YWHAQ 10971
Affinity Capture-MS Homo sapiens
64 NFYC 4802
Affinity Capture-MS Homo sapiens
65 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
66 SMC1A 8243
Cross-Linking-MS (XL-MS) Homo sapiens
67 VAV2 7410
Two-hybrid Homo sapiens
68 CUL2 8453
Affinity Capture-MS Homo sapiens
69 ATP5B 506
Cross-Linking-MS (XL-MS) Homo sapiens
70 TRIM63  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
71 TCAP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
72 C3 718
Cross-Linking-MS (XL-MS) Homo sapiens
73 ACTN1 87
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
74 GAN 8139
Affinity Capture-MS Homo sapiens
75 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
76 RPL10 6134
Cross-Linking-MS (XL-MS) Homo sapiens
77 ATP5I 521
Cross-Linking-MS (XL-MS) Homo sapiens
78 YWHAZ 7534
Cross-Linking-MS (XL-MS) Homo sapiens
79 CRYAB 1410
Affinity Capture-Western Homo sapiens
80 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 NR3C1 2908
Proximity Label-MS Homo sapiens
82 PDIA3 2923
Cross-Linking-MS (XL-MS) Homo sapiens
83 EGFR 1956
Affinity Capture-MS Homo sapiens
84 TOP3A  
Synthetic Lethality Homo sapiens
85 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
86 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
87 WEE1 7465
Synthetic Lethality Homo sapiens
88 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
89 LOC100132735  
Protein-RNA Homo sapiens
90 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ABTB2 25841
Affinity Capture-MS Homo sapiens
92 RAPGEF2 9693
Affinity Capture-MS Homo sapiens
93 MYC  
Affinity Capture-MS Homo sapiens
94 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
95 TOP1 7150
Synthetic Lethality Homo sapiens
96 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
97 HNRNPAB 3182
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
98 DYRK2 8445
Affinity Capture-MS Homo sapiens
99 HYOU1 10525
Cross-Linking-MS (XL-MS) Homo sapiens
100 RNF2  
Affinity Capture-MS Homo sapiens
101 TBCA 6902
Cross-Linking-MS (XL-MS) Homo sapiens
102 MECOM 2122
Affinity Capture-MS Homo sapiens
103 ANKRD23 200539
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
104 RPL26 6154
Cross-Linking-MS (XL-MS) Homo sapiens
105 FHL2 2274
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
106 UBXN1 51035
Cross-Linking-MS (XL-MS) Homo sapiens
107 TPM3 7170
Cross-Linking-MS (XL-MS) Homo sapiens
108 DSP 1832
Cross-Linking-MS (XL-MS) Homo sapiens
109 U2SURP 23350
Cross-Linking-MS (XL-MS) Homo sapiens
110 RNF43  
Proximity Label-MS Homo sapiens
111 FHL1 2273
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
112 PRKCSH 5589
Cross-Linking-MS (XL-MS) Homo sapiens
113 HSPB2  
Two-hybrid Homo sapiens
114 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
115 CPSF6 11052
Co-fractionation Homo sapiens
116 HMGB1 3146
Cross-Linking-MS (XL-MS) Homo sapiens
117 ASF1B  
Affinity Capture-MS Homo sapiens
118 Cep135  
Affinity Capture-MS Mus musculus
119 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
120 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
121 SQSTM1 8878
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
122 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
123 PUF60 22827
Affinity Capture-MS Homo sapiens
124 MTHFD1 4522
Cross-Linking-MS (XL-MS) Homo sapiens
125 TARS 6897
Cross-Linking-MS (XL-MS) Homo sapiens
126 MAP2K1 5604
Synthetic Lethality Homo sapiens
127 LATS1  
Affinity Capture-MS Homo sapiens
128 ESR1  
Affinity Capture-MS Homo sapiens
129 DNAJB11 51726
Cross-Linking-MS (XL-MS) Homo sapiens
130 MYBPC3  
Far Western Homo sapiens
131 EEF1A1P5 158078
Cross-Linking-MS (XL-MS) Homo sapiens
132 NCL 4691
Cross-Linking-MS (XL-MS) Homo sapiens
133 TMEM79  
Affinity Capture-MS Homo sapiens
134 ARRB1 408
Affinity Capture-MS Homo sapiens
135 ANKDD1A  
Affinity Capture-MS Homo sapiens
136 CUL4A 8451
Affinity Capture-MS Homo sapiens
137 C1QBP 708
Proximity Label-MS Homo sapiens
138 SERBP1 26135
Affinity Capture-MS Homo sapiens
139 CUL7 9820
Affinity Capture-MS Homo sapiens
140 CYCS 54205
Cross-Linking-MS (XL-MS) Homo sapiens
141 SPEN 23013
Co-fractionation Homo sapiens
142 CACYBP 27101
Cross-Linking-MS (XL-MS) Homo sapiens
143 NEDD8 4738
Affinity Capture-MS Homo sapiens
144 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here