Gene description for DPM1
Gene name dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
Gene symbol DPM1
Other names/aliases CDGIE
MPDS
Species Homo sapiens
 Database cross references - DPM1
ExoCarta ExoCarta_8813
Vesiclepedia VP_8813
Entrez Gene 8813
HGNC 3005
MIM 603503
UniProt O60762  
 DPM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DPM1
Molecular Function
    dolichyl-phosphate-mannose-protein mannosyltransferase activity GO:0004169 IDA
    dolichyl-phosphate beta-D-mannosyltransferase activity GO:0004582 EXP
    dolichyl-phosphate beta-D-mannosyltransferase activity GO:0004582 IBA
    dolichyl-phosphate beta-D-mannosyltransferase activity GO:0004582 IDA
    protein binding GO:0005515 IPI
    D-mannose binding GO:0005537 IEA
    alcohol binding GO:0043178 IEA
    metal ion binding GO:0046872 ISS
Biological Process
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IBA
    GPI anchor biosynthetic process GO:0006506 IBA
    GPI anchor biosynthetic process GO:0006506 IDA
    GPI anchor biosynthetic process GO:0006506 IDA
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    dolichol metabolic process GO:0019348 IDA
    dolichol metabolic process GO:0019348 IDA
    GDP-mannose metabolic process GO:0019673 IEA
    protein mannosylation GO:0035268 IDA
    protein O-linked mannosylation GO:0035269 IBA
    protein O-linked mannosylation GO:0035269 IDA
    response to oxygen levels GO:0070482 IEA
    dolichol phosphate mannose biosynthetic process GO:0180047 ISS
Subcellular Localization
    nucleus GO:0005634 HDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    dolichol-phosphate-mannose synthase complex GO:0033185 IDA
    dolichol-phosphate-mannose synthase complex GO:0033185 IPI
 Experiment description of studies that identified DPM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DPM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-Western Homo sapiens
2 Cep131  
Affinity Capture-MS Mus musculus
3 BRF2  
Affinity Capture-MS Homo sapiens
4 CLPP 8192
Proximity Label-MS Homo sapiens
5 ATG16L1 55054
Affinity Capture-MS Homo sapiens
6 Cct4 12464
Affinity Capture-MS Mus musculus
7 DPM3 54344
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
8 AATK  
Affinity Capture-MS Homo sapiens
9 TMED10 10972
Affinity Capture-MS Homo sapiens
10 SPRTN  
Affinity Capture-MS Homo sapiens
11 KIF14 9928
Affinity Capture-MS Homo sapiens
12 M6pr 17113
Affinity Capture-MS Mus musculus
13 UBE2H 7328
Affinity Capture-MS Homo sapiens
14 FBXL6  
Affinity Capture-MS Homo sapiens
15 TMPO 7112
Affinity Capture-MS Homo sapiens
16 AMBRA1  
Affinity Capture-MS Homo sapiens
17 FAS 355
Proximity Label-MS Homo sapiens
18 TUBG1 7283
Affinity Capture-MS Homo sapiens
19 RC3H2  
Affinity Capture-MS Homo sapiens
20 KLF16  
Affinity Capture-MS Homo sapiens
21 TDO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 BSG 682
Affinity Capture-MS Homo sapiens
23 UFL1 23376
Affinity Capture-MS Homo sapiens
24 ZBTB16  
Two-hybrid Homo sapiens
25 FOXA1  
Affinity Capture-MS Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
27 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
28 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
29 TFCP2 7024
Affinity Capture-MS Homo sapiens
30 KPNA2 3838
Cross-Linking-MS (XL-MS) Homo sapiens
31 RB1CC1 9821
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 FBXO6 26270
Affinity Capture-MS Homo sapiens
34 FASN 2194
Negative Genetic Homo sapiens
35 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
36 ASXL1  
Affinity Capture-MS Homo sapiens
37 RIPK4  
Affinity Capture-MS Homo sapiens
38 SOX2  
Affinity Capture-MS Homo sapiens
39 ELMSAN1  
Affinity Capture-MS Homo sapiens
40 USP36  
Affinity Capture-MS Homo sapiens
41 LOC100132735  
Protein-RNA Homo sapiens
42 AURKB 9212
Affinity Capture-MS Homo sapiens
43 KIAA1429 25962
Affinity Capture-MS Homo sapiens
44 WWP2 11060
Affinity Capture-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 TBC1D32 221322
Proximity Label-MS Homo sapiens
47 TRIM28 10155
Affinity Capture-MS Homo sapiens
48 B3GNT2 10678
Affinity Capture-MS Homo sapiens
49 MYC  
Affinity Capture-MS Homo sapiens
50 YAP1 10413
Affinity Capture-MS Homo sapiens
51 C16orf72 29035
Affinity Capture-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 MCM2 4171
Affinity Capture-MS Homo sapiens
54 ARNT 405
Affinity Capture-MS Homo sapiens
55 BRD1 23774
Affinity Capture-MS Homo sapiens
56 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 EP300 2033
Affinity Capture-MS Homo sapiens
58 PRKACA 5566
Affinity Capture-MS Homo sapiens
59 DPM2 8818
Co-purification Homo sapiens
60 DDRGK1 65992
Affinity Capture-MS Homo sapiens
61 BMI1  
Affinity Capture-MS Homo sapiens
62 ILK 3611
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 HECTD1 25831
Affinity Capture-MS Homo sapiens
64 ATXN3 4287
Affinity Capture-MS Homo sapiens
65 PHF12  
Affinity Capture-MS Homo sapiens
66 MYCN  
Affinity Capture-MS Homo sapiens
67 ARAF 369
Affinity Capture-MS Homo sapiens
68 C9orf72  
Affinity Capture-MS Homo sapiens
69 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 Ubr5  
Affinity Capture-MS Mus musculus
71 HIF1A 3091
Affinity Capture-MS Homo sapiens
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