Gene description for PIP
Gene name prolactin-induced protein
Gene symbol PIP
Other names/aliases GCDFP-15
GCDFP15
GPIP4
Species Homo sapiens
 Database cross references - PIP
ExoCarta ExoCarta_5304
Entrez Gene 5304
HGNC 8993
MIM 176720
UniProt P12273  
 PIP identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for PIP
Molecular Function
    actin binding GO:0003779 NAS
    glycoprotein binding GO:0001948 IPI
    IgG binding GO:0019864 IDA
    protein binding GO:0005515 IPI
    aspartic-type endopeptidase activity GO:0004190 IMP
    protein dimerization activity GO:0046983 IDA
Biological Process
    proteolysis GO:0006508 IMP
    negative regulation of T cell apoptotic process GO:0070233 IMP
    transmembrane transport GO:0055085 TAS
    retina homeostasis GO:0001895 IEP
    regulation of immune system process GO:0002682 IEA
    positive regulation of gene expression GO:0010628 IDA
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    apical plasma membrane GO:0016324 IEA
    extracellular space GO:0005615 IDA
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified PIP in exosomes
1
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PIP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD4 920
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PIP is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome





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