Gene description for TMEM109
Gene name transmembrane protein 109
Gene symbol TMEM109
Other names/aliases -
Species Homo sapiens
 Database cross references - TMEM109
ExoCarta ExoCarta_79073
Vesiclepedia VP_79073
Entrez Gene 79073
HGNC 28771
UniProt Q9BVC6  
 TMEM109 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Thymus 23844026    
 Gene ontology annotations for TMEM109
Molecular Function
    protein binding GO:0005515 IPI
    voltage-gated monoatomic cation channel activity GO:0022843 ISS
Biological Process
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IBA
    negative regulation of programmed cell death GO:0043069 IMP
    cellular response to gamma radiation GO:0071480 IBA
    cellular response to gamma radiation GO:0071480 IMP
    monoatomic cation transmembrane transport GO:0098655 IEA
Subcellular Localization
    nuclear outer membrane GO:0005640 IEA
    sarcoplasmic reticulum membrane GO:0033017 ISS
    monoatomic ion channel complex GO:0034702 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TMEM109 in exosomes
1
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMEM109
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TOMM40 10452
Co-fractionation Homo sapiens
2 ERGIC3 51614
Two-hybrid Homo sapiens
3 FAS 355
Proximity Label-MS Homo sapiens
4 TMEM52B 120939
Two-hybrid Homo sapiens
5 H2AFJ 55766
Cross-Linking-MS (XL-MS) Homo sapiens
6 GJD3  
Proximity Label-MS Homo sapiens
7 Rab5c 19345
Affinity Capture-MS Mus musculus
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 GPX8 493869
Two-hybrid Homo sapiens
10 AQP2 359
Two-hybrid Homo sapiens
11 VDAC3 7419
Co-fractionation Homo sapiens
12 FGFR1 2260
Affinity Capture-MS Homo sapiens
13 RPN2 6185
Proximity Label-MS Homo sapiens
14 FAP 2191
Affinity Capture-MS Homo sapiens
15 TMEM80  
Two-hybrid Homo sapiens
16 METTL7A 25840
Proximity Label-MS Homo sapiens
17 Psmb4 19172
Affinity Capture-MS Mus musculus
18 MARCKS 4082
Proximity Label-MS Homo sapiens
19 LAMP3  
Proximity Label-MS Homo sapiens
20 TMEM63B 55362
Affinity Capture-MS Homo sapiens
21 SDHA 6389
Affinity Capture-MS Homo sapiens
22 RNF170 81790
Two-hybrid Homo sapiens
23 KIAA1715 80856
Proximity Label-MS Homo sapiens
24 MTIF3  
Two-hybrid Homo sapiens
25 AURKA 6790
Affinity Capture-MS Homo sapiens
26 CCRL2  
Affinity Capture-MS Homo sapiens
27 LMNB1 4001
Proximity Label-MS Homo sapiens
28 GOLT1B 51026
Affinity Capture-MS Homo sapiens
29 EFNA1  
Affinity Capture-MS Homo sapiens
30 BCAP31 10134
Proximity Label-MS Homo sapiens
31 HSD3B7 80270
Proximity Label-MS Homo sapiens
32 ATL3 25923
Affinity Capture-MS Homo sapiens
33 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
34 HSD17B11 51170
Proximity Label-MS Homo sapiens
35 EMD 2010
Proximity Label-MS Homo sapiens
36 NIPAL1  
Affinity Capture-MS Homo sapiens
37 TFCP2 7024
Affinity Capture-MS Homo sapiens
38 STOM 2040
Two-hybrid Homo sapiens
39 TMEM43 79188
Affinity Capture-MS Homo sapiens
40 SOX2  
Affinity Capture-MS Homo sapiens
41 MMGT1 93380
Affinity Capture-MS Homo sapiens
42 Rassf1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
43 SLC18A2  
Two-hybrid Homo sapiens
44 Ppp2ca 19052
Affinity Capture-MS Mus musculus
45 GDE1 51573
Affinity Capture-MS Homo sapiens
46 MGST1 4257
Co-fractionation Homo sapiens
47 B3GAT1  
Proximity Label-MS Homo sapiens
48 SPINT1 6692
Two-hybrid Homo sapiens
49 RAB2A 5862
Affinity Capture-MS Homo sapiens
50 YAP1 10413
Affinity Capture-MS Homo sapiens
51 ATP2A1 487
Proximity Label-MS Homo sapiens
52 MGST3 4259
Two-hybrid Homo sapiens
53 MCM2 4171
Affinity Capture-MS Homo sapiens
54 CRYAB 1410
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
55 CYB5R3 1727
Co-fractionation Homo sapiens
56 PDZK1IP1 10158
Two-hybrid Homo sapiens
57 RPN1 6184
Proximity Label-MS Homo sapiens
58 RAB5C 5878
Proximity Label-MS Homo sapiens
59 GOLM1 51280
Two-hybrid Homo sapiens
60 SSR1 6745
Proximity Label-MS Homo sapiens
61 LMNA 4000
Proximity Label-MS Homo sapiens
62 SEC62 7095
Proximity Label-MS Homo sapiens
63 HSD17B2 3294
Affinity Capture-MS Homo sapiens
64 SEC61B 10952
Proximity Label-MS Homo sapiens
65 TOMM5  
Co-fractionation Homo sapiens
66 CCDC47 57003
Affinity Capture-MS Homo sapiens
67 DERL1 79139
Proximity Label-MS Homo sapiens
68 AQP9  
Two-hybrid Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM109 is involved
No pathways found





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