Gene description for CPNE2
Gene name copine II
Gene symbol CPNE2
Other names/aliases COPN2
CPN2
Species Homo sapiens
 Database cross references - CPNE2
ExoCarta ExoCarta_221184
Vesiclepedia VP_221184
Entrez Gene 221184
HGNC 2315
MIM 604206
UniProt Q96FN4  
 CPNE2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CPNE2
Molecular Function
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    cellular response to calcium ion GO:0071277 IBA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CPNE2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CPNE2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 Skil  
Two-hybrid Mus musculus
3 MCM7 4176
Affinity Capture-MS Homo sapiens
4 Sgol2  
Affinity Capture-MS Mus musculus
5 CNTNAP3  
Affinity Capture-MS Homo sapiens
6 RABEP2 79874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 NUP62 23636
Affinity Capture-MS Homo sapiens
9 NUPL1 9818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 USP9X 8239
Affinity Capture-MS Homo sapiens
12 POLR2I 5438
Affinity Capture-MS Homo sapiens
13 TERF2IP 54386
Two-hybrid Homo sapiens
14 Actb 11461
Two-hybrid Mus musculus
15 CDKN1A  
Two-hybrid Homo sapiens
16 SEPT9 10801
Affinity Capture-MS Homo sapiens
17 Cenpf  
Two-hybrid Mus musculus
18 MLLT4 4301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MCM2 4171
Affinity Capture-MS Homo sapiens
20 CT55  
Two-hybrid Homo sapiens
21 Ppp5c  
Two-hybrid Mus musculus
22 EWSR1 2130
Affinity Capture-MS Homo sapiens
23 NEK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 Bcor  
Two-hybrid Mus musculus
25 Naa11  
Affinity Capture-MS Mus musculus
26 TFCP2 7024
Affinity Capture-MS Homo sapiens
27 MYCN  
Affinity Capture-MS Homo sapiens
28 GBP7  
Two-hybrid Homo sapiens
29 RABGEF1 27342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ANXA7 310
Two-hybrid Homo sapiens
31 DDX39A 10212
Co-fractionation Homo sapiens
32 NCKIPSD 51517
Two-hybrid Homo sapiens
33 PIBF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 Ube2o 217342
Two-hybrid Mus musculus
35 SUPT5H 6829
Affinity Capture-MS Homo sapiens
36 CERS2 29956
Affinity Capture-MS Homo sapiens
37 BVES  
Affinity Capture-MS Homo sapiens
38 TXLNG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 UBA3 9039
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 YAP1 10413
Affinity Capture-MS Homo sapiens
41 Rdx 19684
Two-hybrid Mus musculus
42 COPS5 10987
Affinity Capture-MS Homo sapiens
43 SYNC  
Affinity Capture-MS Homo sapiens
44 MEOX2  
Two-hybrid Homo sapiens
45 EBF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DNM1L 10059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 HSPB8 26353
Affinity Capture-MS Homo sapiens
48 FRMPD2  
Affinity Capture-MS Homo sapiens
49 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 POLR2B 5431
Affinity Capture-MS Homo sapiens
51 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
52 ANLN 54443
Affinity Capture-MS Homo sapiens
53 ANAPC13 25847
Affinity Capture-MS Homo sapiens
54 Mycbp2 105689
Two-hybrid Mus musculus
55 KATNBL1  
Two-hybrid Homo sapiens
56 MEI4  
Two-hybrid Homo sapiens
57 RBM47 54502
Affinity Capture-MS Homo sapiens
58 S100A16 140576
Co-fractionation Homo sapiens
59 RNF138  
Affinity Capture-MS Homo sapiens
60 POLR2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 POLR2A 5430
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 POLR2M  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 DMAP1 55929
Affinity Capture-MS Homo sapiens
64 MYC  
Affinity Capture-MS Homo sapiens
65 USP24 23358
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SORBS3 10174
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 HIP1 3092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 Cdca5  
Affinity Capture-MS Mus musculus
69 EFHC2  
Two-hybrid Homo sapiens
70 EIF3H 8667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TXLNA 200081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 NUP54 53371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 BKRF1  
Affinity Capture-MS
74 ZCCHC7  
Affinity Capture-MS Homo sapiens
75 HDGF 3068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 POLR2J  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SEC23B 10483
Co-fractionation Homo sapiens
78 IKBKG 8517
Reconstituted Complex Homo sapiens
79 RPAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PIGK 10026
Affinity Capture-MS Homo sapiens
81 ZDHHC23  
Affinity Capture-MS Homo sapiens
82 NEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 ZNF317 57693
Affinity Capture-MS Homo sapiens
84 OSBPL3 26031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 NOC2L 26155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 TCF4  
Two-hybrid Homo sapiens
87 THAP4 51078
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
88 SERBP1 26135
Affinity Capture-MS Homo sapiens
89 INTS3 65123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 DDI2 84301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ZNF318 24149
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 LNX1  
Two-hybrid Homo sapiens
93 ZDBF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CPNE2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here