Gene description for AZGP1
Gene name alpha-2-glycoprotein 1, zinc-binding
Gene symbol AZGP1
Other names/aliases ZA2G
ZAG
Species Homo sapiens
 Database cross references - AZGP1
ExoCarta ExoCarta_563
Entrez Gene 563
HGNC 910
MIM 194460
UniProt P25311  
 AZGP1 identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 19199708    
Serum 25265333    
Serum 25265333    
Urine 21557262    
 Gene ontology annotations for AZGP1
Molecular Function
    protein binding GO:0005515 IPI
    glycoprotein binding GO:0001948 IPI
    protein transmembrane transporter activity GO:0008320 NAS
    peptide antigen binding GO:0042605 IEA
    antigen binding GO:0003823 IBA
    ribonuclease activity GO:0004540 NAS
Biological Process
    immune response GO:0006955 IEA
    cell adhesion GO:0007155 ISS
    retina homeostasis GO:0001895 IEP
    negative regulation of cell proliferation GO:0008285 NAS
    transmembrane transport GO:0055085 TAS
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IDA
    antigen processing and presentation GO:0019882 IBA
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 IEA
    protein transmembrane transport GO:0071806 NAS
    RNA phosphodiester bond hydrolysis GO:0090501 NAS
Subcellular Localization
    extracellular space GO:0005615 IDA
    extracellular exosome GO:0070062 IDA
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 IBA
    MHC class I protein complex GO:0042612 IEA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified AZGP1 in exosomes
1
Experiment ID 266
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 267
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 268
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 269
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Serum
Sample name Serum - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 270
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Serum
Sample name Serum - HBV infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 114
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors Li Y, Zhang Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AZGP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGAV 3685
Invitro Homo sapiens
2 AZGP1 563
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AZGP1 is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome





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