Gene description for P2RX4
Gene name purinergic receptor P2X, ligand gated ion channel, 4
Gene symbol P2RX4
Other names/aliases P2X4
P2X4R
Species Homo sapiens
 Database cross references - P2RX4
ExoCarta ExoCarta_5025
Vesiclepedia VP_5025
Entrez Gene 5025
HGNC 8535
MIM 600846
UniProt Q99571  
 P2RX4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for P2RX4
Molecular Function
    purinergic nucleotide receptor activity GO:0001614 IDA
    purinergic nucleotide receptor activity GO:0001614 IMP
    extracellularly ATP-gated monoatomic cation channel activity GO:0004931 IBA
    extracellularly ATP-gated monoatomic cation channel activity GO:0004931 IDA
    signaling receptor binding GO:0005102 ISS
    copper ion binding GO:0005507 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IC
    zinc ion binding GO:0008270 ISS
    identical protein binding GO:0042802 IPI
    cadherin binding GO:0045296 IPI
    ligand-gated calcium channel activity GO:0099604 IDA
    ligand-gated calcium channel activity GO:0099604 ISS
Biological Process
    tissue homeostasis GO:0001894 NAS
    regulation of sodium ion transport GO:0002028 ISS
    response to ischemia GO:0002931 ISS
    signal transduction GO:0007165 IDA
    regulation of blood pressure GO:0008217 IMP
    positive regulation of calcium ion transport into cytosol GO:0010524 IDA
    positive regulation of calcium ion transport into cytosol GO:0010524 IMP
    negative regulation of cardiac muscle hypertrophy GO:0010614 IMP
    neuronal action potential GO:0019228 IEA
    sensory perception of pain GO:0019233 ISS
    calcium-mediated signaling GO:0019722 TAS
    positive regulation of prostaglandin secretion GO:0032308 NAS
    response to ATP GO:0033198 IDA
    monoatomic ion transmembrane transport GO:0034220 IDA
    response to fluid shear stress GO:0034405 IDA
    purinergic nucleotide receptor signaling pathway GO:0035590 IMP
    endothelial cell activation GO:0042118 TAS
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 NAS
    behavioral response to pain GO:0048266 ISS
    response to axon injury GO:0048678 ISS
    positive regulation of calcium-mediated signaling GO:0050850 IDA
    positive regulation of calcium-mediated signaling GO:0050850 IMP
    regulation of chemotaxis GO:0050920 ISS
    sensory perception of touch GO:0050975 ISS
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    membrane depolarization GO:0051899 IDA
    positive regulation of calcium ion transport GO:0051928 NAS
    regulation of cardiac muscle contraction GO:0055117 IMP
    relaxation of cardiac muscle GO:0055119 IMP
    excitatory postsynaptic potential GO:0060079 IEA
    calcium ion transmembrane transport GO:0070588 IBA
    calcium ion transmembrane transport GO:0070588 IDA
    cellular response to zinc ion GO:0071294 ISS
    cellular response to ATP GO:0071318 IDA
    apoptotic signaling pathway GO:0097190 IDA
    regulation of ruffle assembly GO:1900027 ISS
    positive regulation of microglial cell migration GO:1904141 ISS
    positive regulation of microglial cell migration GO:1904141 TAS
    positive regulation of endothelial cell chemotaxis GO:2001028 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IC
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    cell junction GO:0030054 IDA
    neuronal cell body GO:0043025 IEA
    terminal bouton GO:0043195 IEA
    dendritic spine GO:0043197 IEA
    cell body GO:0044297 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
    parallel fiber to Purkinje cell synapse GO:0098688 IEA
    postsynaptic density membrane GO:0098839 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified P2RX4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for P2RX4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 KRTAP12-3  
Two-hybrid Homo sapiens
3 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PIH1D3  
Two-hybrid Homo sapiens
5 ADCK4 79934
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ORC4  
Affinity Capture-MS Homo sapiens
7 DNAJC19 131118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GALNT1 2589
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KCNJ8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CDH5  
Affinity Capture-Western Homo sapiens
11 EMC7 56851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 EIF2B2 8892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PLD6  
Affinity Capture-MS Homo sapiens
14 WNT4  
Two-hybrid Homo sapiens
15 HS2ST1 9653
Affinity Capture-MS Homo sapiens
16 TNPO3 23534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 EMC1 23065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KRTAP5-9  
Two-hybrid Homo sapiens
19 KRTAP1-1  
Two-hybrid Homo sapiens
20 TMEM246 84302
Affinity Capture-MS Homo sapiens
21 OPRL1 4987
Affinity Capture-MS Homo sapiens
22 EIF2B5 8893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FAM20B 9917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 LRP10 26020
Affinity Capture-MS Homo sapiens
25 FDFT1 2222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SAAL1 113174
Affinity Capture-MS Homo sapiens
27 MKRN2 23609
Affinity Capture-RNA Homo sapiens
28 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MCM2 4171
Two-hybrid Homo sapiens
30 GLT8D1 55830
Affinity Capture-MS Homo sapiens
31 LRRC8E 80131
Affinity Capture-MS Homo sapiens
32 EIF2B3 8891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FGFR1 2260
Affinity Capture-MS Homo sapiens
34 EIF2B1 1967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ARMCX3 51566
Affinity Capture-MS Homo sapiens
36 PIGH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 THEM6  
Affinity Capture-MS Homo sapiens
38 C17orf62 79415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SPTLC2 9517
Affinity Capture-MS Homo sapiens
40 B3GALT5  
Affinity Capture-MS Homo sapiens
41 C3orf52  
Affinity Capture-MS Homo sapiens
42 EIF2B4 8890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 NBPF19  
Two-hybrid Homo sapiens
44 GALNT14  
Affinity Capture-MS Homo sapiens
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