Gene description for MYO9B
Gene name myosin IXB
Gene symbol MYO9B
Other names/aliases CELIAC4
MYR5
Species Homo sapiens
 Database cross references - MYO9B
ExoCarta ExoCarta_4650
Entrez Gene 4650
HGNC 7609
MIM 602129
UniProt Q13459  
 MYO9B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for MYO9B
Molecular Function
    ATPase activity GO:0016887 IDA
    actin filament binding GO:0051015 IEA
    calmodulin binding GO:0005516 IDA
    zinc ion binding GO:0008270 IEA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    ATP-dependent protein binding GO:0043008 IEA
    actin-dependent ATPase activity GO:0030898 IEA
    ADP binding GO:0043531 IDA
    microfilament motor activity GO:0000146 IDA
    ATP binding GO:0005524 IDA
    protein homodimerization activity GO:0042803 IDA
    actin binding GO:0003779 IDA
Biological Process
    establishment of cell polarity GO:0030010 IEA
    metabolic process GO:0008152 IEA
    actin filament-based movement GO:0030048 TAS
    lamellipodium morphogenesis GO:0072673 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    Rho protein signal transduction GO:0007266 IC
    positive regulation of GTPase activity GO:0043547 IEA
    monocyte chemotaxis GO:0002548 IEA
    ARF protein signal transduction GO:0032011 IDA
    macrophage chemotaxis GO:0048246 IEA
Subcellular Localization
    lamellipodium GO:0030027 IEA
    ruffle GO:0001726 IEA
    actin cytoskeleton GO:0015629 TAS
    cytosol GO:0005829 TAS
    filamentous actin GO:0031941 IDA
    cell cortex GO:0005938 IDA
    filopodium tip GO:0032433 IEA
    membrane GO:0016020 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    myosin complex GO:0016459 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified MYO9B in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYO9B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRC41  
Two-hybrid Homo sapiens
2 CALM1 801
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MYO9B is involved
PathwayEvidenceSource
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Rho GTPase cycle TAS Reactome





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