Gene description for EIF4G1
Gene name eukaryotic translation initiation factor 4 gamma, 1
Gene symbol EIF4G1
Other names/aliases EIF-4G1
EIF4F
EIF4G
EIF4GI
P220
PARK18
Species Homo sapiens
 Database cross references - EIF4G1
ExoCarta ExoCarta_1981
Vesiclepedia VP_1981
Entrez Gene 1981
HGNC 3296
MIM 600495
UniProt Q04637  
 EIF4G1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Saliva 19199708    
T lymphocytes 34108659    
 Gene ontology annotations for EIF4G1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 TAS
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IDA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    translation factor activity, RNA binding GO:0008135 IMP
    eukaryotic initiation factor 4E binding GO:0008190 IDA
    eukaryotic initiation factor 4E binding GO:0008190 TAS
    translation initiation factor binding GO:0031369 TAS
    molecular adaptor activity GO:0060090 TAS
Biological Process
    behavioral fear response GO:0001662 IEA
    cap-dependent translational initiation GO:0002191 TAS
    translation GO:0006412 IMP
    translation GO:0006412 IPI
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IDA
    translational initiation GO:0006413 NAS
    translational initiation GO:0006413 TAS
    regulation of translational initiation GO:0006446 IMP
    regulation of translational initiation GO:0006446 NAS
    macromolecule biosynthetic process GO:0009059 IGI
    negative regulation of autophagy GO:0010507 IMP
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IMP
    neuron differentiation GO:0030182 IEA
    positive regulation of cell growth GO:0030307 IMP
    lung development GO:0030324 IEA
    cellular response to nutrient levels GO:0031669 IMP
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 IDA
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 TAS
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IMP
    response to ethanol GO:0045471 IEA
    positive regulation of neuron differentiation GO:0045666 IEA
    positive regulation of protein metabolic process GO:0051247 IMP
    regulation of cellular response to stress GO:0080135 TAS
    energy homeostasis GO:0097009 IMP
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IMP
    positive regulation of protein localization to cell periphery GO:1904377 IGI
    positive regulation of eukaryotic translation initiation factor 4F complex assembly GO:1905537 IMP
    positive regulation of eukaryotic translation initiation factor 4F complex assembly GO:1905537 IPI
    regulation of presynapse assembly GO:1905606 IGI
    positive regulation of mRNA cap binding GO:1905612 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IMP
    cytoplasmic stress granule GO:0010494 IDA
    membrane GO:0016020 HDA
    eukaryotic translation initiation factor 4F complex GO:0016281 IBA
    eukaryotic translation initiation factor 4F complex GO:0016281 IDA
    eukaryotic translation initiation factor 4F complex GO:0016281 NAS
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified EIF4G1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
23
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
34
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
36
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for EIF4G1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 EDEM1  
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 EIF4G3 8672
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
6 CDC26 246184
Affinity Capture-MS Homo sapiens
7 ISG15 9636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KLF8  
Affinity Capture-MS Homo sapiens
9 NCBP1 4686
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 LGR4 55366
Affinity Capture-MS Homo sapiens
11 QARS 5859
Co-fractionation Homo sapiens
12 SLFN11 91607
Proximity Label-MS Homo sapiens
13 EIF3C 8663
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 PTBP1 5725
Co-fractionation Homo sapiens
15 KIAA0020 9933
Co-fractionation Homo sapiens
16 PSMA4 5685
Co-fractionation Homo sapiens
17 CTBP2 1488
Two-hybrid Homo sapiens
18 RACGAP1 29127
Affinity Capture-MS Homo sapiens
19 TARDBP 23435
Affinity Capture-MS Homo sapiens
20 FMR1 2332
Affinity Capture-MS Homo sapiens
21 BTF3 689
Affinity Capture-MS Homo sapiens
22 MKNK2 2872
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
23 BRCA1 672
Co-fractionation Homo sapiens
24 UBE3A 7337
Two-hybrid Homo sapiens
25 ENKD1  
Two-hybrid Homo sapiens
26 PPP2R1A 5518
Co-fractionation Homo sapiens
27 EIF4A1 1973
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 CIB1 10519
Two-hybrid Homo sapiens
29 EIF4EBP2 1979
Affinity Capture-MS Homo sapiens
30 PSMB8 5696
Co-fractionation Homo sapiens
31 GCHFR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TMPO 7112
Affinity Capture-MS Homo sapiens
33 TGM2 7052
Co-fractionation Homo sapiens
34 APEX1 328
Affinity Capture-RNA Homo sapiens
35 KIAA1429 25962
Affinity Capture-MS Homo sapiens
36 RNH1 6050
Co-fractionation Homo sapiens
37 CAPZB 832
Affinity Capture-MS Homo sapiens
38 CENPU  
Two-hybrid Homo sapiens
39 TOP2A 7153
Affinity Capture-MS Homo sapiens
40 POLDIP3 84271
Affinity Capture-Western Homo sapiens
41 EMILIN1 11117
Affinity Capture-MS Homo sapiens
42 B3GNT2 10678
Affinity Capture-MS Homo sapiens
43 DYRK1A 1859
Affinity Capture-MS Homo sapiens
44 KIF23 9493
Affinity Capture-MS Homo sapiens
45 MCM2 4171
Affinity Capture-MS Homo sapiens
46 DCTN4 51164
Co-fractionation Homo sapiens
47 USP3 9960
Affinity Capture-MS Homo sapiens
48 KIF5B 3799
Co-fractionation Homo sapiens
49 SPAG9 9043
Co-fractionation Homo sapiens
50 EIF3B 8662
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 METAP2 10988
Affinity Capture-MS Homo sapiens
52 MAMDC2 256691
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 IQGAP1 8826
Co-fractionation Homo sapiens
54 NPM1 4869
Affinity Capture-MS Homo sapiens
55 PSMD12 5718
Co-fractionation Homo sapiens
56 PRIM1  
Co-fractionation Homo sapiens
57 EEF1E1 9521
Co-fractionation Homo sapiens
58 SRP19 6728
Affinity Capture-MS Homo sapiens
59 SF3A2 8175
Co-fractionation Homo sapiens
60 ANGEL1  
Affinity Capture-MS Homo sapiens
61 GSPT1 2935
Affinity Capture-MS Homo sapiens
62 MKI67  
Cross-Linking-MS (XL-MS) Homo sapiens
63 EIF3E 3646
Affinity Capture-MS Homo sapiens
64 NFATC2IP  
Affinity Capture-MS Homo sapiens
65 ITGA4 3676
Affinity Capture-MS Homo sapiens
66 SRP68 6730
Affinity Capture-MS Homo sapiens
67 VCAM1 7412
Affinity Capture-MS Homo sapiens
68 DDX6 1656
Affinity Capture-MS Homo sapiens
69 SFN 2810
Co-fractionation Homo sapiens
70 Gspt1 14852
Affinity Capture-MS Mus musculus
71 CALR 811
Co-fractionation Homo sapiens
72 MECP2 4204
Affinity Capture-MS Homo sapiens
73 Eif3a 13669
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
74 AIMP2 7965
Co-fractionation Homo sapiens
75 RBM42  
Affinity Capture-MS Homo sapiens
76 ZFYVE9  
Two-hybrid Homo sapiens
77 TNKS1BP1 85456
Co-fractionation Homo sapiens
78 PABPC4 8761
Co-fractionation Homo sapiens
79 PSMC3 5702
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
80 RBM8A 9939
Affinity Capture-MS Homo sapiens
81 KIF14 9928
Affinity Capture-MS Homo sapiens
82 HSPA8 3312
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
83 EMC9  
Affinity Capture-MS Homo sapiens
84 UNK  
Affinity Capture-RNA Homo sapiens
85 E2F4  
Affinity Capture-MS Homo sapiens
86 LRRC59 55379
Cross-Linking-MS (XL-MS) Homo sapiens
87 Taok2  
Affinity Capture-MS Mus musculus
88 DLD 1738
Affinity Capture-MS Homo sapiens
89 EIF3K 27335
Affinity Capture-MS Homo sapiens
90 RPS16 6217
Affinity Capture-MS Homo sapiens
91 Eif4e  
Affinity Capture-Western Mus musculus
92 RNF4 6047
Affinity Capture-MS Homo sapiens
93 USP10 9100
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
94 PSMC4 5704
Co-fractionation Homo sapiens
95 CUL4B 8450
Affinity Capture-MS Homo sapiens
96 PARK2  
Affinity Capture-MS Homo sapiens
97 SCG3  
Cross-Linking-MS (XL-MS) Homo sapiens
98 DRG1 4733
Affinity Capture-MS Homo sapiens
99 MSX2  
Affinity Capture-MS Homo sapiens
100 SRSF3 6428
Affinity Capture-Western Homo sapiens
101 PSMD11 5717
Co-fractionation Homo sapiens
102 MAPRE1 22919
Affinity Capture-MS Homo sapiens
103 PSMD14 10213
Co-fractionation Homo sapiens
104 Pabpc1 18458
Affinity Capture-MS Mus musculus
105 RPL5 6125
Affinity Capture-MS Homo sapiens
106 HIF1AN 55662
Affinity Capture-MS Homo sapiens
107 PABPC1 26986
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
108 CDH1 999
Proximity Label-MS Homo sapiens
109 C9orf72  
Affinity Capture-MS Homo sapiens
110 MYH9 4627
Co-fractionation Homo sapiens
111 VCP 7415
Affinity Capture-MS Homo sapiens
112 NTRK1 4914
Affinity Capture-MS Homo sapiens
113 LRPPRC 10128
Co-fractionation Homo sapiens
114 RPS10 6204
Co-fractionation Homo sapiens
115 GK  
Two-hybrid Homo sapiens
116 P4HB 5034
Co-fractionation Homo sapiens
117 RPA4  
Proximity Label-MS Homo sapiens
118 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
119 PSMC2 5701
Co-fractionation Homo sapiens
120 CTPS1 1503
Co-fractionation Homo sapiens
121 EIF4G2 1982
Co-fractionation Homo sapiens
122 HECTD1 25831
Affinity Capture-MS Homo sapiens
123 POLE  
Co-fractionation Homo sapiens
124 IFT22 64792
Affinity Capture-MS Homo sapiens
125 Eif4e 117045
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
126 MEX3A  
Affinity Capture-RNA Homo sapiens
127 NUDC 10726
Cross-Linking-MS (XL-MS) Homo sapiens
128 CYLD  
Affinity Capture-MS Homo sapiens
129 SF3A3 10946
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
130 WDYHV1  
Two-hybrid Homo sapiens
131 EIF4A2 1974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
132 PHLPP1  
Proximity Label-MS Homo sapiens
133 HNRNPD 3184
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
134 EIF3G 8666
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
135 HSPA5 3309
Affinity Capture-MS Homo sapiens
136 ITPR2 3709
Cross-Linking-MS (XL-MS) Homo sapiens
137 AHCY 191
Co-fractionation Homo sapiens
138 HTRA2 27429
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
139 OTUD1 220213
Affinity Capture-MS Homo sapiens
140 C9orf78 51759
Affinity Capture-MS Homo sapiens
141 RC3H2  
Affinity Capture-MS Homo sapiens
142 SIRT7  
Affinity Capture-MS Homo sapiens
143 NELFCD 51497
Affinity Capture-MS Homo sapiens
144 CCDC57 284001
Two-hybrid Homo sapiens
145 NAT10 55226
Co-fractionation Homo sapiens
146 MRPL9 65005
Affinity Capture-MS Homo sapiens
147 U2AF2 11338
Cross-Linking-MS (XL-MS) Homo sapiens
148 SIRPA 140885
Cross-Linking-MS (XL-MS) Homo sapiens
149 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 CEP350 9857
Affinity Capture-MS Homo sapiens
151 NUP43 348995
Proximity Label-MS Homo sapiens
152 EIF4E 1977
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
153 FOXL1  
Affinity Capture-MS Homo sapiens
154 MYC  
Affinity Capture-MS Homo sapiens
155 FN1 2335
Affinity Capture-MS Homo sapiens
156 PUS7 54517
Co-fractionation Homo sapiens
157 SF3A1 10291
Co-fractionation Homo sapiens
158 PRKCI 5584
Co-fractionation Homo sapiens
159 PDCD4 27250
Affinity Capture-Western Homo sapiens
160 SGTB  
Affinity Capture-MS Homo sapiens
161 LINC00152  
Affinity Capture-RNA Homo sapiens
162 NCAPH 23397
Affinity Capture-MS Homo sapiens
163 ACTR5 79913
Affinity Capture-MS Homo sapiens
164 PSPC1 55269
Affinity Capture-MS Homo sapiens
165 FBL 2091
Co-fractionation Homo sapiens
166 FXR2 9513
Two-hybrid Homo sapiens
167 CNTROB  
Affinity Capture-MS Homo sapiens
168 YWHAQ 10971
Co-fractionation Homo sapiens
169 DLST 1743
Affinity Capture-MS Homo sapiens
170 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
171 ATPAF2 91647
Two-hybrid Homo sapiens
172 SKIV2L2 23517
Co-fractionation Homo sapiens
173 MAGEA6  
Affinity Capture-MS Homo sapiens
174 RPS11 6205
Affinity Capture-MS Homo sapiens
175 SF3B3 23450
Co-fractionation Homo sapiens
176 CDKN2D  
Two-hybrid Homo sapiens
177 MSN 4478
Co-fractionation Homo sapiens
178 HAT1 8520
Co-fractionation Homo sapiens
179 Eif3e 16341
Affinity Capture-MS Mus musculus
180 CLCN2  
Affinity Capture-MS Homo sapiens
181 EIF3M 10480
Affinity Capture-MS Homo sapiens
182 CCDC8  
Affinity Capture-MS Homo sapiens
183 EIF3D 8664
Affinity Capture-MS Homo sapiens
184 EIF3H 8667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
185 AIMP1 9255
Co-fractionation Homo sapiens
186 HSP90AA1 3320
Co-fractionation Homo sapiens
187 FTL 2512
Co-fractionation Homo sapiens
188 PEF1 553115
Two-hybrid Homo sapiens
189 EIF3F 8665
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
190 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
191 FASN 2194
Positive Genetic Homo sapiens
192 EIF4EBP1  
Affinity Capture-MS Homo sapiens
193 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
194 RPS3 6188
Co-fractionation Homo sapiens
195 CHAF1B  
Affinity Capture-MS Homo sapiens
196 MBD3 53615
Cross-Linking-MS (XL-MS) Homo sapiens
197 MKNK1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
198 EIF3I 8668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
199 WARS 7453
Co-fractionation Homo sapiens
200 FOXB1  
Affinity Capture-MS Homo sapiens
201 ATG13 9776
Affinity Capture-MS Homo sapiens
202 PCBP1 5093
Proximity Label-MS Homo sapiens
203 PDHA1 5160
Affinity Capture-MS Homo sapiens
204 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
205 HNRNPM 4670
Cross-Linking-MS (XL-MS) Homo sapiens
206 YWHAB 7529
Co-fractionation Homo sapiens
207 MAGEL2  
Proximity Label-MS Homo sapiens
208 G3BP1 10146
Affinity Capture-MS Homo sapiens
209 SRP72 6731
Affinity Capture-MS Homo sapiens
210 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
211 G3BP2 9908
Affinity Capture-MS Homo sapiens
212 TOR1AIP1 26092
Affinity Capture-MS Homo sapiens
213 DDX58 23586
Affinity Capture-RNA Homo sapiens
214 TARS 6897
Cross-Linking-MS (XL-MS) Homo sapiens
215 OTUD6B 51633
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
216 KLF16  
Affinity Capture-MS Homo sapiens
217 MTERF4  
Cross-Linking-MS (XL-MS) Homo sapiens
218 ESR1  
Affinity Capture-MS Homo sapiens
219 PDZD8 118987
Affinity Capture-MS Homo sapiens
220 UFL1 23376
Affinity Capture-MS Homo sapiens
221 NUP50 10762
Co-fractionation Homo sapiens
222 CDV3 55573
Cross-Linking-MS (XL-MS) Homo sapiens
223 EIF3J 8669
Affinity Capture-Western Homo sapiens
224 RAB31 11031
Co-fractionation Homo sapiens
225 EIF4B 1975
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
226 CAD 790
Co-fractionation Homo sapiens
227 RAD21 5885
Affinity Capture-Western Homo sapiens
228 CIT 11113
Affinity Capture-MS Homo sapiens
229 SERBP1 26135
Affinity Capture-MS Homo sapiens
230 CUL7 9820
Affinity Capture-MS Homo sapiens
231 KRAS 3845
Negative Genetic Homo sapiens
232 HNRNPU 3192
Cross-Linking-MS (XL-MS) Homo sapiens
233 SLBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
234 SRP9 6726
Affinity Capture-MS Homo sapiens
235 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
236 ATXN3 4287
Affinity Capture-MS Homo sapiens
237 VIM 7431
Co-fractionation Homo sapiens
238 KRT34  
Two-hybrid Homo sapiens
239 PRMT1 3276
Affinity Capture-MS Homo sapiens
240 A1CF  
Two-hybrid Homo sapiens
241 EIF5 1983
Affinity Capture-MS Homo sapiens
242 PSMD3 5709
Co-fractionation Homo sapiens
243 EIF3L 51386
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EIF4G1 is involved
PathwayEvidenceSource
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA TAS Reactome
Axon guidance IEA Reactome
Cap-dependent Translation Initiation TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Deadenylation of mRNA TAS Reactome
Deadenylation of mRNA IEA Reactome
Deadenylation-dependent mRNA decay TAS Reactome
Deadenylation-dependent mRNA decay IEA Reactome
Developmental Biology IEA Reactome
Eukaryotic Translation Initiation TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Immune System TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
M-decay: degradation of maternal mRNAs by maternally stored factors IEA Reactome
Maternal to zygotic transition (MZT) IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of RNA TAS Reactome
Metabolism of RNA IEA Reactome
MTOR signalling TAS Reactome
mTORC1-mediated signalling TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements TAS Reactome
Ribosomal scanning and start codon recognition TAS Reactome
Signal Transduction TAS Reactome
Signaling by ROBO receptors IEA Reactome
Translation TAS Reactome
Translation initiation complex formation TAS Reactome
Z-decay: degradation of maternal mRNAs by zygotically expressed factors IEA Reactome





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