Gene description for PYGM
Gene name phosphorylase, glycogen, muscle
Gene symbol PYGM
Other names/aliases -
Species Homo sapiens
 Database cross references - PYGM
ExoCarta ExoCarta_5837
Vesiclepedia VP_5837
Entrez Gene 5837
HGNC 9726
MIM 608455
UniProt P11217  
 PYGM identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PYGM
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    glycogen phosphorylase activity GO:0008184 IBA
    glycogen phosphorylase activity GO:0008184 IDA
    glycogen phosphorylase activity GO:0008184 IMP
    pyridoxal phosphate binding GO:0030170 IBA
    maltodextrin phosphorylase activity GO:0031220 IEA
Biological Process
    glycogen metabolic process GO:0005977 TAS
    glycogen catabolic process GO:0005980 IBA
    glycogen catabolic process GO:0005980 IDA
    glycogen catabolic process GO:0005980 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PYGM in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PYGM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DSN1 79980
Affinity Capture-MS Homo sapiens
2 Ccdc15  
Affinity Capture-MS Mus musculus
3 LATS1  
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
6 MECOM 2122
Affinity Capture-MS Homo sapiens
7 MCM2 4171
Affinity Capture-MS Homo sapiens
8 HAGH 3029
Affinity Capture-MS Homo sapiens
9 FANCD2  
Affinity Capture-MS Homo sapiens
10 ANKRD55  
Affinity Capture-MS Homo sapiens
11 MYEOV  
Affinity Capture-MS Homo sapiens
12 TM4SF18  
Affinity Capture-MS Homo sapiens
13 MCM5 4174
Affinity Capture-MS Homo sapiens
14 TSPAN33 340348
Affinity Capture-MS Homo sapiens
15 EPB41L5 57669
Affinity Capture-MS Homo sapiens
16 TAF6L 10629
Affinity Capture-MS Homo sapiens
17 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LZTR1 8216
Affinity Capture-MS Homo sapiens
19 FKBP6  
Affinity Capture-MS Homo sapiens
20 PACSIN3 29763
Affinity Capture-MS Homo sapiens
21 GPRASP2  
Affinity Capture-MS Homo sapiens
22 RDH11 51109
Affinity Capture-MS Homo sapiens
23 DPPA3  
Affinity Capture-MS Homo sapiens
24 DEGS1  
Affinity Capture-MS Homo sapiens
25 RSPH6A  
Affinity Capture-MS Homo sapiens
26 FAM81B  
Affinity Capture-MS Homo sapiens
27 TSPO 706
Affinity Capture-MS Homo sapiens
28 BRCA2 675
Affinity Capture-MS Homo sapiens
29 ABTB2 25841
Affinity Capture-MS Homo sapiens
30 DDHD1 80821
Affinity Capture-MS Homo sapiens
31 WDYHV1  
Two-hybrid Homo sapiens
32 FDPS 2224
Affinity Capture-MS Homo sapiens
33 PYGB 5834
Affinity Capture-MS Homo sapiens
34 TRIM31  
Affinity Capture-MS Homo sapiens
35 SPIN1  
Affinity Capture-MS Homo sapiens
36 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
37 TOP1 7150
Protein-peptide Homo sapiens
38 PPP2CA 5515
Biochemical Activity Homo sapiens
39 ARL16  
Affinity Capture-MS Homo sapiens
40 GBE1 2632
Co-fractionation Homo sapiens
41 SPAST 6683
Affinity Capture-MS Homo sapiens
42 DNAJC3 5611
Affinity Capture-MS Homo sapiens
43 STBD1 8987
Affinity Capture-MS Homo sapiens
44 MRPS31  
Affinity Capture-MS Homo sapiens
45 S100A1 6271
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 PRKAB2 5565
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 DNAJC30  
Affinity Capture-MS Homo sapiens
48 TAF1B  
Affinity Capture-MS Homo sapiens
49 MAGEF1  
Affinity Capture-MS Homo sapiens
50 RYBP  
Affinity Capture-MS Homo sapiens
51 TRIM63  
Two-hybrid Homo sapiens
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