Gene description for TUBA4B
Gene name tubulin, alpha 4b
Gene symbol TUBA4B
Other names/aliases TUBA4
Species Homo sapiens
 Database cross references - TUBA4B
ExoCarta ExoCarta_80086
Entrez Gene 80086
HGNC 18637
 TUBA4B identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 17956143    
 Gene ontology annotations for TUBA4B
Molecular Function
    GTPase activity GO:0003924 IEA
    molecular_function GO:0003674 ND
    structural constituent of cytoskeleton GO:0005200 IEA
    GTP binding GO:0005525 IEA
Biological Process
    biological_process GO:0008150 ND
    metabolic process GO:0008152 IEA
    microtubule-based process GO:0007017 IEA
Subcellular Localization
    microtubule GO:0005874 IEA
    cellular_component GO:0005575 ND
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified TUBA4B in exosomes
1
Experiment ID 76
ISEV standards
EM
EV Biophysical techniques
TSG101|GAPDH|HSP90
EV Cytosolic markers
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TUBA4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TUBA4B is involved
No pathways found





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