Gene description for CASP14
Gene name caspase 14, apoptosis-related cysteine peptidase
Gene symbol CASP14
Other names/aliases -
Species Homo sapiens
 Database cross references - CASP14
ExoCarta ExoCarta_23581
Vesiclepedia VP_23581
Entrez Gene 23581
HGNC 1502
MIM 605848
UniProt P31944  
 CASP14 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Mesenchymal stem cells Unpublished / Not applicable
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CASP14
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IEA
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    epidermis development GO:0008544 TAS
    keratinization GO:0031424 TAS
    positive regulation of neuron apoptotic process GO:0043525 IBA
    cornification GO:0070268 TAS
Subcellular Localization
    cornified envelope GO:0001533 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    keratin filament GO:0045095 IEA
 Experiment description of studies that identified CASP14 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 267
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CASP14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CASP8 841
Affinity Capture-Western Homo sapiens
3 WWP1 11059
Affinity Capture-MS Homo sapiens
4 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
5 RPS19BP1  
Affinity Capture-MS Homo sapiens
6 EBF2  
Affinity Capture-MS Homo sapiens
7 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 AZGP1 563
Affinity Capture-MS Homo sapiens
9 KIAA1429 25962
Affinity Capture-MS Homo sapiens
10 WTAP 9589
Affinity Capture-MS Homo sapiens
11 CASP1  
Affinity Capture-Western Homo sapiens
12 B3GNT2 10678
Affinity Capture-MS Homo sapiens
13 MCM2 4171
Affinity Capture-MS Homo sapiens
14 E2F6 1876
Affinity Capture-MS Homo sapiens
15 LIAS  
Affinity Capture-MS Homo sapiens
16 FLG 2312
Affinity Capture-MS Homo sapiens
17 KPRP 448834
Affinity Capture-MS Homo sapiens
18 UCHL5 51377
Reconstituted Complex Homo sapiens
19 SFN 2810
Affinity Capture-MS Homo sapiens
20 METTL23  
Affinity Capture-MS Homo sapiens
21 IFNA4  
Affinity Capture-MS Homo sapiens
22 MYCN  
Affinity Capture-MS Homo sapiens
23 USP53 54532
Affinity Capture-MS Homo sapiens
24 NDUFS5 4725
Affinity Capture-MS Homo sapiens
25 GSK3B 2932
Affinity Capture-MS Homo sapiens
26 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SLCO6A1  
Affinity Capture-MS Homo sapiens
28 CHMP4C 92421
Affinity Capture-MS Homo sapiens
29 ZNF550  
Affinity Capture-MS Homo sapiens
30 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
31 POF1B 79983
Affinity Capture-MS Homo sapiens
32 DSG1 1828
Affinity Capture-MS Homo sapiens
33 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 ARHGEF40 55701
Affinity Capture-MS Homo sapiens
36 ARG1 383
Affinity Capture-MS Homo sapiens
37 ITLN2  
Affinity Capture-MS Homo sapiens
38 TRIM21 6737
Affinity Capture-MS Homo sapiens
39 SARAF  
Affinity Capture-MS Homo sapiens
40 S100A8 6279
Affinity Capture-MS Homo sapiens
41 SPRR2E  
Affinity Capture-MS Homo sapiens
42 MEOX2  
Two-hybrid Homo sapiens
43 ALDH3A1 218
Affinity Capture-MS Homo sapiens
44 APTX  
Affinity Capture-MS Homo sapiens
45 DDX39B 7919
Affinity Capture-MS Homo sapiens
46 PRKAB1 5564
Affinity Capture-MS Homo sapiens
47 SETD7 80854
Affinity Capture-MS Homo sapiens
48 CYLD  
Affinity Capture-MS Homo sapiens
49 C1orf68 100129271
Affinity Capture-MS Homo sapiens
50 CASP4  
Affinity Capture-Western Homo sapiens
51 ZRANB1 54764
Affinity Capture-MS Homo sapiens
52 SERPINB3 6317
Affinity Capture-MS Homo sapiens
53 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
54 IVL 3713
Affinity Capture-MS Homo sapiens
55 NDST2  
Affinity Capture-MS Homo sapiens
56 FANCD2  
Affinity Capture-MS Homo sapiens
57 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
58 METTL14  
Affinity Capture-MS Homo sapiens
59 CCNC  
Affinity Capture-MS Homo sapiens
60 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 JADE1  
Affinity Capture-MS Homo sapiens
62 MOGAT3  
Affinity Capture-MS Homo sapiens
63 CD274 29126
Affinity Capture-MS Homo sapiens
64 ZSCAN12  
Affinity Capture-MS Homo sapiens
65 S100A7 6278
Affinity Capture-MS Homo sapiens
66 ZC3HC1  
Affinity Capture-MS Homo sapiens
67 FTH1 2495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SERPINB5 5268
Affinity Capture-MS Homo sapiens
69 Ect2  
Affinity Capture-MS Mus musculus
70 ARHGAP29 9411
Affinity Capture-MS Homo sapiens
71 MYC  
Affinity Capture-MS Homo sapiens
72 COA5  
Affinity Capture-MS Homo sapiens
73 CASP10  
Affinity Capture-Western Homo sapiens
74 BLMH 642
Affinity Capture-MS Homo sapiens
75 UBE2J2 118424
Affinity Capture-MS Homo sapiens
76 MRPL38  
Affinity Capture-MS Homo sapiens
77 C18orf21  
Affinity Capture-MS Homo sapiens
78 STX17 55014
Affinity Capture-MS Homo sapiens
79 PFDN5 5204
Affinity Capture-MS Homo sapiens
80 RIPPLY3  
Affinity Capture-MS Homo sapiens
81 CCDC8  
Affinity Capture-MS Homo sapiens
82 DSP 1832
Affinity Capture-MS Homo sapiens
83 PRSS16 10279
Affinity Capture-MS Homo sapiens
84 JUP 3728
Affinity Capture-MS Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 SLC25A6 293
Affinity Capture-MS Homo sapiens
87 CASP2  
Affinity Capture-Western Homo sapiens
88 CUL2 8453
Affinity Capture-MS Homo sapiens
89 AMTN  
Affinity Capture-MS Homo sapiens
90 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 DDX3X 1654
Affinity Capture-MS Homo sapiens
92 CTSD 1509
Affinity Capture-MS Homo sapiens
93 ZNF154  
Affinity Capture-MS Homo sapiens
94 CCR1  
Affinity Capture-MS Homo sapiens
95 SOX4  
Proximity Label-MS Homo sapiens
96 DSC1 1823
Affinity Capture-MS Homo sapiens
97 C1orf35  
Affinity Capture-MS Homo sapiens
98 PTDSS1 9791
Affinity Capture-MS Homo sapiens
99 OTUB1 55611
Affinity Capture-MS Homo sapiens
100 CDSN 1041
Affinity Capture-MS Homo sapiens
101 S100A9 6280
Affinity Capture-MS Homo sapiens
102 PKP1 5317
Affinity Capture-MS Homo sapiens
103 Plekhg2  
Affinity Capture-MS Mus musculus
104 ESR1  
Affinity Capture-MS Homo sapiens
105 ERCC3  
Affinity Capture-MS Homo sapiens
106 COQ2  
Affinity Capture-MS Homo sapiens
107 GTF2H3  
Affinity Capture-MS Homo sapiens
108 TGM3 7053
Affinity Capture-MS Homo sapiens
109 CALML5 51806
Affinity Capture-MS Homo sapiens
110 SERPINB12 89777
Affinity Capture-MS Homo sapiens
111 TSPAN6 7105
Affinity Capture-MS Homo sapiens
112 PINK1  
Affinity Capture-MS Homo sapiens
113 GPR18  
Affinity Capture-MS Homo sapiens
114 GOT1 2805
Affinity Capture-MS Homo sapiens
115 TCF3  
Affinity Capture-MS Homo sapiens
116 METTL3  
Affinity Capture-MS Homo sapiens
117 ZUFSP 221302
Affinity Capture-MS Homo sapiens
118 PRKX 5613
Affinity Capture-MS Homo sapiens
119 OR2A4 79541
Affinity Capture-MS Homo sapiens
120 ANXA2 302
Affinity Capture-MS Homo sapiens
121 PHF8  
Affinity Capture-MS Homo sapiens
122 GALNT9  
Affinity Capture-MS Homo sapiens
123 CUL4A 8451
Affinity Capture-MS Homo sapiens
124 FLG2 388698
Affinity Capture-MS Homo sapiens
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