Gene description for TAF3
Gene name TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
Gene symbol TAF3
Other names/aliases TAF140
TAFII-140
TAFII140
Species Homo sapiens
 Database cross references - TAF3
ExoCarta ExoCarta_83860
Vesiclepedia VP_83860
Entrez Gene 83860
HGNC 17303
MIM 606576
UniProt Q5VWG9  
 TAF3 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TAF3
Molecular Function
    p53 binding GO:0002039 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IC
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IEA
    transcription regulator inhibitor activity GO:0140416 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    mRNA transcription by RNA polymerase II GO:0042789 IDA
    negative regulation of DNA-binding transcription factor activity GO:0043433 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    RNA polymerase II preinitiation complex assembly GO:0051123 IPI
    RNA polymerase II preinitiation complex assembly GO:0051123 ISS
    maintenance of protein location in nucleus GO:0051457 IDA
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
Subcellular Localization
    male germ cell nucleus GO:0001673 IEA
    nucleus GO:0005634 EXP
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IBA
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIID complex GO:0005669 IPI
    transcription factor TFIID complex GO:0005669 ISS
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified TAF3 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TAF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 YY1 7528
Proximity Label-MS Homo sapiens
3 NAP1L4 4676
Co-fractionation Homo sapiens
4 TAF6 6878
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
5 TAF5  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 TAF7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 KLF8  
Proximity Label-MS Homo sapiens
9 FASN 2194
Negative Genetic Homo sapiens
10 EFHD1  
Affinity Capture-MS Homo sapiens
11 PARP1 142
Proximity Label-MS Homo sapiens
12 ELF1 1997
Proximity Label-MS Homo sapiens
13 MYOD1  
Proximity Label-MS Homo sapiens
14 FEV  
Proximity Label-MS Homo sapiens
15 SAP130  
Affinity Capture-Western Homo sapiens
16 MZT1  
Affinity Capture-MS Homo sapiens
17 ELK3  
Proximity Label-MS Homo sapiens
18 ELF4 2000
Proximity Label-MS Homo sapiens
19 KLF3  
Proximity Label-MS Homo sapiens
20 POLD1 5424
Affinity Capture-MS Homo sapiens
21 KPNB1 3837
Reconstituted Complex Homo sapiens
22 CTCF  
Affinity Capture-Western Homo sapiens
23 PHIP 55023
Affinity Capture-MS Homo sapiens
24 BRD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CDC23 8697
Co-fractionation Homo sapiens
26 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
27 KPNA2 3838
Reconstituted Complex Homo sapiens
28 TAF10 6881
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
29 SPATA25  
Affinity Capture-MS Homo sapiens
30 TAF2  
Affinity Capture-MS Homo sapiens
31 TAF1  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NUP50 10762
Proximity Label-MS Homo sapiens
33 NIFK 84365
Proximity Label-MS Homo sapiens
34 HIST3H3 8290
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
35 KLF16  
Proximity Label-MS Homo sapiens
36 CENPA  
Proximity Label-MS Homo sapiens
37 TBP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 HNF4A  
Proximity Label-MS Homo sapiens
39 KHDRBS2  
Affinity Capture-MS Homo sapiens
40 DDX23 9416
Proximity Label-MS Homo sapiens
41 TAF11  
Affinity Capture-MS Homo sapiens
42 CDC16 8881
Co-fractionation Homo sapiens
43 TAF9 6880
Affinity Capture-MS Homo sapiens
44 TAF4 6874
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
45 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
46 TAF12  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
47 RPL31 6160
Proximity Label-MS Homo sapiens
48 ZCCHC10  
Affinity Capture-MS Homo sapiens
49 ANAPC10  
Co-fractionation Homo sapiens
50 TAF13  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
51 ELF2  
Proximity Label-MS Homo sapiens
52 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
53 MAGEA9  
Affinity Capture-MS Homo sapiens
54 MICAL1 64780
Affinity Capture-MS Homo sapiens
55 TAF9B  
Affinity Capture-MS Homo sapiens
56 PAX6  
Proximity Label-MS Homo sapiens
57 TBPL2  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
58 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
59 BRD3 8019
Affinity Capture-MS Homo sapiens
60 MCM2 4171
Cross-Linking-MS (XL-MS) Homo sapiens
61 TAF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 KAT2A  
Affinity Capture-Western Homo sapiens
63 TAF7L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 HIST2H4A 8370
Affinity Capture-Western Homo sapiens
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