Gene description for GLUD1
Gene name glutamate dehydrogenase 1
Gene symbol GLUD1
Other names/aliases GDH
GDH1
GLUD
Species Homo sapiens
 Database cross references - GLUD1
ExoCarta ExoCarta_2746
Entrez Gene 2746
HGNC 4335
MIM 138130
UniProt P00367  
 GLUD1 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 19415654    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GLUD1
Molecular Function
    protein binding GO:0005515 IPI
    leucine binding GO:0070728 IDA
    identical protein binding GO:0042802 TAS
    ATP binding GO:0005524 IEA
    ADP binding GO:0043531 IDA
    glutamate dehydrogenase [NAD(P)+] activity GO:0004353 IDA
    GTP binding GO:0005525 IDA
    glutamate dehydrogenase (NAD+) activity GO:0004352 IDA
    NAD+ binding GO:0070403 IDA
Biological Process
    substantia nigra development GO:0021762 IEP
    glutamate biosynthetic process GO:0006537 IDA
    glutamine metabolic process GO:0006541 ISS
    glutamate catabolic process GO:0006538 IDA
    positive regulation of insulin secretion GO:0032024 IMP
    small molecule metabolic process GO:0044281 TAS
    oxidation-reduction process GO:0055114 IEA
    cellular amino acid biosynthetic process GO:0008652 TAS
    tricarboxylic acid metabolic process GO:0072350 ISS
    cellular nitrogen compound metabolic process GO:0034641 TAS
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
    mitochondrion GO:0005739 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified GLUD1 in exosomes
1
Experiment ID 65
ISEV standards
✘ EV Biophysical techniques
✔
HSP60|HSP27|GAPDH
EV Cytosolic markers
✔
FLOT2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors Staubach S, Razawi H, Hanisch FG.
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GLUD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GLUD1 is involved
PathwayEvidenceSource
Amino acid synthesis and interconversion (transamination) TAS Reactome





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