Gene description for PSME1
Gene name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Gene symbol PSME1
Other names/aliases IFI5111
PA28A
PA28alpha
REGalpha
Species Homo sapiens
 Database cross references - PSME1
ExoCarta ExoCarta_5720
Entrez Gene 5720
HGNC 9568
MIM 600654
UniProt Q06323  
 PSME1 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PSME1
Molecular Function
    endopeptidase activator activity GO:0061133 IEA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    innate immune response GO:0045087 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    programmed cell death GO:0012501 TAS
    gene expression GO:0010467 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 TAS
    regulation of cellular amino acid metabolic process GO:0006521 TAS
    positive regulation of endopeptidase activity GO:0010950 IEA
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    mitotic cell cycle GO:0000278 TAS
    apoptotic process GO:0006915 TAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    viral process GO:0016032 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    regulation of apoptotic process GO:0042981 TAS
    protein polyubiquitination GO:0000209 TAS
Subcellular Localization
    cytoplasm GO:0005737 TAS
    nucleoplasm GO:0005654 TAS
    extracellular exosome GO:0070062 IDA
    proteasome complex GO:0000502 TAS
    cytosol GO:0005829 TAS
    proteasome activator complex GO:0008537 IEA
 Experiment description of studies that identified PSME1 in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PSME1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBB2A 7280
Two-hybrid Homo sapiens
2 PSME2 5721
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 EMD  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 PSME1 5720
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 VIM 7431
Two-hybrid Homo sapiens
6 VCL 7414
Two-hybrid Homo sapiens
7 CHD3  
Two-hybrid Homo sapiens
8 EIF6 3692
Two-hybrid Homo sapiens
9 PFDN1  
Two-hybrid Homo sapiens
10 USP22 23326
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PSME1 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
Asymmetric localization of PCP proteins TAS Reactome
AUF1 (hnRNP D0) destabilizes mRNA TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Autodegradation of the E3 ubiquitin ligase COP1 TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDT1 association with the CDC6:ORC:origin complex TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Cross-presentation of soluble exogenous antigens (endosomes) TAS Reactome
Dectin-1 mediated noncanonical NF-kB signaling TAS Reactome
degradation of AXIN TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
degradation of DVL TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Degradation of GLI2 by the proteasome TAS Reactome
ER-Phagosome pathway TAS Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
Hedgehog 'on' state TAS Reactome
Hedgehog ligand biogenesis TAS Reactome
Hh mutants that don't undergo autocatalytic processing are degraded by ERAD TAS Reactome
Orc1 removal from chromatin TAS Reactome
Orc1 removal from chromatin TAS Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation TAS Reactome
Regulation of ornithine decarboxylase (ODC) TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF-beta-TrCP mediated degradation of Emi1 TAS Reactome
Separation of Sister Chromatids TAS Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A TAS Reactome
Ubiquitin-dependent degradation of Cyclin D1 TAS Reactome
Vif-mediated degradation of APOBEC3G TAS Reactome
Vpu mediated degradation of CD4 TAS Reactome





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