Gene description for TPR
Gene name translocated promoter region, nuclear basket protein
Gene symbol TPR
Other names/aliases -
Species Homo sapiens
 Database cross references - TPR
ExoCarta ExoCarta_7175
Entrez Gene 7175
HGNC 12017
MIM 189940
UniProt P12270  
 TPR identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for TPR
Molecular Function
    protein anchor GO:0043495 IMP
    nucleocytoplasmic transporter activity GO:0005487 IDA
    protein homodimerization activity GO:0042803 IDA
    molecular_function GO:0003674 ND
    mRNA binding GO:0003729 IDA
    poly(A) RNA binding GO:0044822 IDA
    heat shock protein binding GO:0031072 IDA
    protein binding GO:0005515 IPI
    transporter activity GO:0005215 IMP
    dynein complex binding GO:0070840 IDA
    tubulin binding GO:0015631 IDA
    mitogen-activated protein kinase binding GO:0051019 IDA
    chromatin binding GO:0003682 IDA
Biological Process
    negative regulation of RNA export from nucleus GO:0046832 IMP
    positive regulation of heterochromatin assembly GO:0031453 IMP
    carbohydrate metabolic process GO:0005975 TAS
    positive regulation of protein export from nucleus GO:0046827 ISS
    mitotic cell cycle GO:0000278 TAS
    viral life cycle GO:0019058 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    viral process GO:0016032 TAS
    protein sumoylation GO:0016925 TAS
    negative regulation of translational initiation GO:0045947 IMP
    biological_process GO:0008150 ND
    regulation of cellular response to heat GO:1900034 TAS
    protein import into nucleus GO:0006606 IMP
    regulation of protein stability GO:0031647 IMP
    regulation of glucose transport GO:0010827 TAS
    nuclear pore organization GO:0006999 IMP
    cellular response to heat GO:0034605 TAS
    hexose transport GO:0008645 TAS
    mRNA export from nucleus in response to heat stress GO:0031990 IDA
    cytokine-mediated signaling pathway GO:0019221 TAS
    RNA import into nucleus GO:0006404 IDA
    MAPK import into nucleus GO:0000189 IMP
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 IMP
    nuclear pore complex assembly GO:0051292 IMP
    mitotic nuclear envelope disassembly GO:0007077 TAS
    RNA export from nucleus GO:0006405 IMP
    nuclear matrix organization GO:0043578 IMP
    regulation of mitotic spindle assembly GO:1901673 IMP
    cellular protein metabolic process GO:0044267 TAS
    post-translational protein modification GO:0043687 TAS
    positive regulation of protein import into nucleus GO:0042307 IMP
    viral transcription GO:0019083 TAS
    regulation of protein import into nucleus GO:0042306 IMP
    cell division GO:0051301 IEA
    positive regulation of intracellular protein transport GO:0090316 IMP
    small molecule metabolic process GO:0044281 TAS
    response to epidermal growth factor GO:0070849 IDA
    cellular response to interferon-alpha GO:0035457 ISS
    regulation of mitotic sister chromatid separation GO:0010965 IMP
    gene expression GO:0010467 TAS
    regulation of mRNA export from nucleus GO:0010793 IMP
    protein export from nucleus GO:0006611 IMP
    mitotic nuclear division GO:0007067 IEA
    glucose transport GO:0015758 TAS
    transmembrane transport GO:0055085 TAS
    regulation of protein export from nucleus GO:0046825 IMP
    mitotic spindle assembly checkpoint GO:0007094 IMP
Subcellular Localization
    mitotic spindle GO:0072686 IDA
    nuclear inclusion body GO:0042405 IDA
    cytoplasmic dynein complex GO:0005868 IDA
    nuclear periphery GO:0034399 IDA
    nuclear membrane GO:0031965 IDA
    nuclear pore GO:0005643 IDA
    nucleoplasm GO:0005654 TAS
    nuclear pore nuclear basket GO:0044615 IDA
    nuclear envelope GO:0005635 IDA
    nucleus GO:0005634 IDA
    extrinsic component of membrane GO:0019898 IDA
    cytoplasm GO:0005737 IDA
    kinetochore GO:0000776 IDA
    cellular_component GO:0005575 ND
 Experiment description of studies that identified TPR in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for TPR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Biochemical Activity Homo sapiens
2 NUP107  
Affinity Capture-Western Homo sapiens
3 NUP153  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 NUP93 9688
Affinity Capture-Western Homo sapiens
5 NUP205 23165
Affinity Capture-Western Homo sapiens
6 NUP98 4928
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here